公开(公告)号：US09822361B2

公开(公告)日：2017-11-21

申请号：US14897577

申请日：2014-06-05

申请人： APSE LLC

发明人： Juan Pedro Humberto Arhancet ,  Juan P. Arhancet ,  Kimberly Delaney ,  Kathleen B. Hall ,  Neena Summers ,  Edward Oates

IPC分类号： C12N15/11 ,  C12N15/113 ,  A61K48/00 ,  A61K9/51

CPC分类号： C12N15/113 ,  A61K9/5184 ,  A61K48/00 ,  C07K14/005 ,  C12N15/111 ,  C12N2310/12 ,  C12N2310/121 ,  C12N2310/14 ,  C12N2310/141 ,  C12N2310/3519 ,  C12N2310/531 ,  C12N2320/32 ,  C12N2330/50 ,  C12N2795/00023 ,  C12N2795/00042 ,  C12N2795/18122 ,  C12N2795/18123 ,  C12N2795/18142

摘要： Novel processes and compositions are described which use viral capsid proteins resistant to hydrolases to prepare virus-like particles to enclose and subsequently isolate and purify target cargo molecules of interest including nucleic acids such as siRNAs and shRNAs, miRNAs, messenger RNAs, small peptides and bioactive molecules.

公开(公告)号：US12102667B2

公开(公告)日：2024-10-01

申请号：US17820316

申请日：2022-08-17

申请人： LyseNTech Co., Ltd. ,  Hankuk University OF Foreign Studies Research & Business Foundation

发明人： Heejoon Myung ,  Min Soo Kim ,  Hye-Won Hong ,  Young Deuk Kim ,  Jaeyeon Jang

IPC分类号： A61K38/47 ,  A61K38/12 ,  A61P31/04 ,  C12N7/00 ,  C12N9/36

CPC分类号： A61K38/47 ,  A61K38/12 ,  A61P31/04 ,  C12N7/00 ,  C12N9/2462 ,  C12Y302/01017 ,  C12N2795/00042

摘要： Provided are a novel polypeptide having endolysin activity, a fusion protein comprising the polypeptide and an antibiotic active protein, and an antibiotic use against a gram-negative pathogen of the polypeptide and/or fusion protein and/or a use for prevention and/or treatment of gram-negative pathogen infection and/or disease or symptoms related to gram negative pathogen infection.

公开(公告)号：US20230330167A1

公开(公告)日：2023-10-19

申请号：US18314755

申请日：2023-05-09

申请人： SNIPR Biome ApS

发明人： Jakob Krause HAABER

IPC分类号： A61K35/76 ,  C12N15/73 ,  C12N7/02 ,  A61K38/16 ,  C07K14/005

CPC分类号： A61K35/76 ,  C12N15/73 ,  C12N7/025 ,  A61K38/162 ,  C07K14/005 ,  A61K38/164 ,  C12N2795/00052 ,  C12N2795/10121 ,  C12N2795/10142 ,  C12N2795/10152 ,  C12N2310/20 ,  C12N2795/00042 ,  C12N2795/00032 ,  C12N2795/10132 ,  C12N2795/10151

摘要： The invention relates to the production of phage and non-replicative transduction particles using DNAs (eg, plasmids and helper phage, mobile genetic elements (MGEs) or plasmids with chromosomally integrated helper phage genes), as well as the phage, helper phage, kits, compositions and methods involving these. The non-replicative transduction particles can be used to deliver antibacterial agents comprising a guided nuclease system.

公开(公告)号：US20230235361A1

公开(公告)日：2023-07-27

申请号：US18001782

申请日：2021-07-05

申请人： Eligo Bioscience

发明人： Xavier DUPORTET ,  Jesus FERNANDEZ RODRIGUEZ

IPC分类号： C12N15/90 ,  C12N15/74 ,  C12N15/11 ,  C12N9/22

CPC分类号： C12N15/902 ,  C12N15/74 ,  C12N15/11 ,  C12N9/22 ,  C12N2310/20 ,  C12N2800/80 ,  C12N2795/00042 ,  C12N2795/00044 ,  C12N2795/00052

摘要： The invention relates to methods, kits, and compositions for reducing the level of or eliminating a nucleic acid vector in situ. The invention encompasses compositions and methods for selectively eradicating nucleic acid vectors in the microbiota using packaged phagemids. The microbiota can be intestinal and the packaged phagemids can be administered orally. The phagemid encodes a nuclease or other enzyme that genetically modifies the nucleic acid vector so that the nucleic acid vector can be inactivated or eliminated.

公开(公告)号：US20190240248A1

公开(公告)日：2019-08-08

申请号：US16208103

申请日：2018-12-03

申请人： ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY ,  NATIONAL CENTER FOR NANOSCIENCE AND TECHNOLOGY

发明人： Hao YAN ,  Qiao JIANG ,  Suping LI ,  Baoquan DING ,  Guangjun NIE

IPC分类号： A61K31/7088 ,  A61K9/00 ,  A61K47/26 ,  C12N7/00 ,  A61K9/51 ,  C12N15/115 ,  A61P35/00

CPC分类号： A61K31/7088 ,  A61K9/0019 ,  A61K9/51 ,  A61K47/26 ,  A61P35/00 ,  B82Y5/00 ,  B82Y40/00 ,  C12N7/00 ,  C12N15/115 ,  C12N2310/16 ,  C12N2795/00022 ,  C12N2795/00032 ,  C12N2795/00042

摘要： In certain embodiments, the present invention provides a DNA nanostructure nanorobot comprising: a single stranded DNA scaffold strand of about 5,000 to 10,000 bases in length; a plurality of staple strands of DNA, wherein each staple strands are about 20 to 40 bases in length, wherein each staple strand has a unique sequence and is hybridized to a specific position on the DNA scaffold strand, wherein the plurality of staple strands hybridized to the DNA scaffold form a sheet having a top surface and a bottom surface; and one or more fastener strands of DNA, wherein the one or more fastener strands of DNA is capable of fastening the sheet into an origami structure.

公开(公告)号：US20160177299A1

公开(公告)日：2016-06-23

申请号：US14897577

申请日：2014-06-05

申请人： APSE LLC

发明人： Juan Pedro Humberto ARHANCET ,  JUAN P. ARHANCET ,  Kimberly DELANEY ,  Kathleen B. HALL ,  Neena SUMMERS ,  Edward OATES

IPC分类号： C12N15/113

CPC分类号： C12N15/113 ,  A61K9/5184 ,  A61K48/00 ,  C07K14/005 ,  C12N15/111 ,  C12N2310/12 ,  C12N2310/121 ,  C12N2310/14 ,  C12N2310/141 ,  C12N2310/3519 ,  C12N2310/531 ,  C12N2320/32 ,  C12N2330/50 ,  C12N2795/00023 ,  C12N2795/00042 ,  C12N2795/18122 ,  C12N2795/18123 ,  C12N2795/18142

摘要： Novel processes and compositions are described which use viral capsid proteins resistant to hydrolases to prepare virus-like particles to enclose and subsequently isolate and purify target cargo molecules of interest including nucleic acids such as siRNAs and shRNAs, miRNAs, messenger RNAs, small peptides and bioactive molecules.

摘要翻译： 描述了新的方法和组合物，其使用对水解酶具有抗性的病毒衣壳蛋白来制备病毒样颗粒以包围并随后分离和纯化目标物质分子，包括核酸如siRNA和shRNA，miRNA，信使RNA，小肽和生物活性 分子。

公开(公告)号：US20240046114A1

公开(公告)日：2024-02-08

申请号：US18355107

申请日：2023-07-19

申请人： The Board of Trustees of the University of Illinois

发明人： Oligica Milenkovic ,  Nagendra Athreya ,  Apratim Khandelwal ,  Jean-Pierre Leburton ,  Xiuling Li ,  Charles Schroeder ,  SeyedKasra Tabatabaei ,  Bo Li

IPC分类号： G06N3/123 ,  C12N7/00

CPC分类号： G06N3/123 ,  C12N7/00 ,  C12N2310/52 ,  C12N2795/00042 ,  C12N2310/3181 ,  C12N2795/00031

摘要： The present disclosure provides systems and methods that can provide portable, real-time accessible DNA memories. An example DNA-based data storage system includes a loading region configured to receive a plurality of DNA-based data storage elements in a suspension fluid and a plurality of microtubes disposed in a capture/release region. The microtubes are configured to capture and release the DNA-based data storage elements. The DNA-based data storage system also includes a linearization region configured to linearize the DNA-based data storage elements and a readout region with a readout device configured to provide information indicative of the respective DNA-based data storage elements.

公开(公告)号：US11755922B2

公开(公告)日：2023-09-12

申请号：US16593450

申请日：2019-10-04

申请人： The Board of Trustees of the University of Illinois

发明人： Olgica Milenkovic ,  Nagendra Athreya ,  Apratim Khandelwal ,  Jean-Pierre Leburton ,  Xiuling Li ,  Charles Schroeder ,  SeyedKasra Tabatabaei ,  Bo Li

IPC分类号： G06N3/00 ,  G06N3/12 ,  G06N3/123 ,  C12N7/00

CPC分类号： G06N3/123 ,  C12N7/00 ,  C12N2310/3181 ,  C12N2310/52 ,  C12N2795/00031 ,  C12N2795/00042

摘要： The present disclosure provides systems and methods that can provide portable, real-time accessible DNA memories. An example DNA-based data storage system includes a loading region configured to receive a plurality of DNA-based data storage elements in a suspension fluid and a plurality of microtubes disposed in a capture/release region. The microtubes are configured to capture and release the DNA-based data storage elements. The DNA-based data storage system also includes a linearization region configured to linearize the DNA-based data storage elements and a readout region with a readout device configured to provide information indicative of the respective DNA-based data storage elements.

公开(公告)号：US20100278846A1

公开(公告)日：2010-11-04

申请号：US12636705

申请日：2009-12-11

申请人： IAN A. FERGUSON

发明人： IAN A. FERGUSON

IPC分类号： A61K39/00 ,  A61P37/04

CPC分类号： C12N7/00 ,  A61K39/00 ,  A61K2039/5256 ,  A61K2039/5258 ,  A61K2039/543 ,  C07K2319/735 ,  C12N2795/00042 ,  C12N2795/00051 ,  C12N2810/85

摘要： Multiple sequential screening tests have been performed on phage display libraries, and polypeptide sequences have been identified that potently drive both: (i) intake into mucosal immune cells, including NALT cells in the nose and throat; and, (ii) phagocytic intake and processing by antigen-presenting cells, such as macrophages. Such polypeptide sequences can be used as potent “target and deliver” components in vaccines that can be administered nasally, or to other mucous membranes. Such vaccines can be made very rapidly and in huge quantities, from bacteriophages that will also carry antigenic sequences in their coat proteins, or other immunoactive components. Alternately, such “target and deliver” polypeptides can be incorporated into vaccines derived from eukaryotic viruses or cellular pathogens. Enhancements also are disclosed, such as agents that can activate one or more types of toll-like receptors, to increase immunes responses and guide them in desired directions.

摘要翻译： 已经对噬菌体展示文库进行了多次连续的筛选测试，并且已经鉴定出有效驱动两者的多肽序列：（i）摄入到粘膜免疫细胞，包括鼻和喉部的NALT细胞; 和（ii）由抗原呈递细胞如巨噬细胞吞噬摄入和加工。 这样的多肽序列可以用作可以被鼻腔或其它粘膜施用的疫苗中的有效的“靶和递送”成分。 这样的疫苗可以非常迅速地并且大量地从还会在其外壳蛋白或其它免疫活性成分中携带抗原序列的噬菌体制备。 或者，这样的“靶和递送”多肽可以并入来自真核病毒或细胞病原体的疫苗中。 还公开了增强剂，例如可以激活一种或多种类型的toll样受体的药剂，以增加免疫反应并将它们引导到期望的方向。

公开(公告)号：US20090317324A1

公开(公告)日：2009-12-24

申请号：US12279689

申请日：2007-02-15

申请人： Beka Solomon ,  Rachel Cohen Kupiec

发明人： Beka Solomon ,  Rachel Cohen Kupiec

IPC分类号： A61K51/10 ,  C12N7/00 ,  A61K39/395 ,  A61P25/00

CPC分类号： C12N15/1037 ,  A61K39/0007 ,  A61K2039/543 ,  C07K16/18 ,  C07K16/26 ,  C07K2317/77 ,  C07K2319/735 ,  C12N7/00 ,  C12N2795/00042

摘要： The present invention relates to a phage display vehicle composed of a filamentous bacteriophage displaying on its surface, as a non-filamentous bacteriophage molecule, protein A or a fragment or variant thereof capable of binding the Fc portion of antibodies, and an antibody or an antigen-antibody immunocomplex bound to protein A or a fragment or variant thereof by its Fc portion. The phage display vehicle is formulated into a pharmaceutical composition and can be used to treat/inhibit or to diagnose a brain disease, disorder or condition.

摘要翻译： 本发明涉及一种噬菌体展示载体，其由表面上显示为非丝状噬菌体分子的蛋白质A或能够结合抗体Fc部分的片段或变体的丝状噬菌体和抗体或抗原 - 抗体免疫复合物通过其Fc部分与蛋白A或其片段或变体结合。 将噬菌体展示载体配制成药物组合物，可用于治疗/抑制或诊断脑疾病，病症或病症。