公开(公告)号：US20160138071A1

公开(公告)日：2016-05-19

申请号：US14898374

申请日：2014-06-16

申请人： PILOT POINT LLC

发明人： Stephen C. Edberg

IPC分类号： C12Q1/06 ,  G01N35/00 ,  G01N21/49 ,  G01N35/04 ,  G01N21/25 ,  C12Q1/04 ,  G01N35/02

CPC分类号： C12Q1/06 ,  C12Q1/04 ,  G01N21/251 ,  G01N21/253 ,  G01N21/49 ,  G01N21/51 ,  G01N21/6486 ,  G01N35/00871 ,  G01N35/025 ,  G01N35/04 ,  G01N2035/00891 ,  G01N2035/0401 ,  G01N2201/061

摘要： A method and instrument for detecting the presence or absence of a target organism in a test sample is provided. The instrument includes a carousel, a light source, alight detector, and a control system. The carousel is configured to hold a plurality of test sample containers, with each container held by the carousel at a container position. Each of the containers holds an admixture of a test mixture inoculated with the test sample. The light detector is operable to detect light emitted by the light source that has passed through the test sample disposed within the test container. The control system includes a processor. The control system is adapted to produce information indicative of the presence or absence of the target organism in the test sample using signals produced by the light detector.

摘要翻译： 提供了用于检测测试样品中目标生物体存在或不存在的方法和仪器。 该仪器包括转盘，光源，检测器和控制系统。 转盘被构造成容纳多个测试样本容器，每个容器由转盘保持在容器位置。 每个容器保持接种测试样品的测试混合物的混合物。 光检测器可操作以检测已经通过设置在测试容器内的测试样品的光源发射的光。 控制系统包括处理器。 控制系统适于使用由光检测器产生的信号产生指示测试样品中目标生物体存在或不存在的信息。

公开(公告)号：US20110269163A1

公开(公告)日：2011-11-03

申请号：US13181173

申请日：2011-07-12

申请人： Stephen C. Edberg

发明人： Stephen C. Edberg

IPC分类号： C12Q1/37

CPC分类号： C12Q1/14

摘要： A presence/absence test for Staphylococcus aureus (S. aureus) involves placing a first generation test sample in a solution that will clot in the presence of S. aureus. The solution contains components that will selectively grow S. aureus and also contains clotting factors that will react with S. aureus, if S. aureus is present in the sample, to clot the solution. Examples of specimen samples that can be tested include nasal swabs and lesion swabs, among others. The test can also be modified to detect the presence or absence of methicillin resistant S. Aureus (MRSA).

公开(公告)号：US09512462B2

公开(公告)日：2016-12-06

申请号：US14357489

申请日：2011-11-10

申请人： Stephen C. Edberg

发明人： Stephen C. Edberg

IPC分类号： C12Q1/14 ,  C12Q1/56

CPC分类号： C12Q1/14 ,  C12Q1/56 ,  G01N2333/31 ,  G01N2333/952

摘要： A method for detecting the presence or absence of an antibiotic resistant pathogenic staphylococci in a test sample is provided. The method includes the steps of: a) providing a test medium having at least one hydrolysable substrate, which substrate is operable to promote the growth of the antibiotic resistant pathogenic staphylococci and to produce a detectable signal when the hydrolyzable substrate is metabolized by the antibiotic resistant pathogenic staphylococci; b) forming a mixture of the test sample and hydrated test medium; c) incubating the mixture at temperatures in the range of about 20° C. to about 35° C.; and d) detecting the presence or absence of the antibiotic resistant pathogenic staphylococci in the test sample by the presence or absence of the detectable signal in the mixture.

摘要翻译： 提供了一种在测试样品中检测抗生素抗性致病性葡萄球菌的存在或不存在的方法。 该方法包括以下步骤：a）提供具有至少一个可水解底物的测试介质，该底物可操作以促进抗生素抗性致病性葡萄球菌的生长，并且当可水解底物被抗生素抗性代谢时产生可检测信号 致病性葡萄球菌; b）形成测试样品和水合测试介质的混合物; c）在约20℃至约35℃的温度范围内孵育该混合物; 和d）通过混合物中可检测信号的存在或不存在来检测测试样品中抗生素抗性致病葡萄球菌的存在或不存在。

公开(公告)号：US09404141B2

公开(公告)日：2016-08-02

申请号：US14304481

申请日：2014-06-13

申请人： Pilots Point LLC

发明人： Stephen C. Edberg

IPC分类号： C12Q1/04 ,  C12Q1/06 ,  G01N21/78 ,  C12Q1/14 ,  C12Q1/10 ,  C12Q1/34

CPC分类号： C12Q1/06 ,  C12Q1/04 ,  C12Q1/045 ,  C12Q1/10 ,  C12Q1/14 ,  C12Q1/34 ,  C12Q2304/00 ,  G01N21/78

摘要： A method of detecting the presence or absence of a target microbe in a test sample is provided. The method includes the steps of: a) providing a test mixture that includes organic micro particles in a form that promotes the formation of a microbial biofilm, operative amounts of essential vitamins and elements needed to support growth of the target microbe, and a metabolizable substrate which can be metabolized by the target microbe t the extent needed to support continued reproductive growth thereof, and which cannot be metabolized by other viable microbes in the test sample, whereupon a sensible characteristic of the sample is altered when the substrate is metabolized; b) providing a test sample obtained from a biological, environmental, or food source, and combining the test sample in unprocessed form with the test mixture to form an admixture: and c) detecting the presence or absence of target microbes in the sample based on the presence or absence of the detectable characteristic. The micro particles are in a relative amount within the test mixture that is effective to accelerate the formation of microbial biofilms within the admixture of the test sample and the test mixture.

摘要翻译： 提供了一种在测试样品中检测目标微生物的存在或不存在的方法。 该方法包括以下步骤：a）提供包括促进微生物生物膜形成的形式的有机微粒的测试混合物，必需维生素的有效量和支持靶微生物生长所必需的元件，以及代谢底物 其可以被目标微生物代谢以支持其继续繁殖生长所需的程度，并且其不能被测试样品中的其他活的微生物代谢，于是当基质被代谢时，样品的显性特征被改变; b）提供从生物，环境或食物来源获得的测试样品，并将未处理形式的测试样品与测试混合物组合以形成混合物;以及c）基于以下方式检测样品中目标微生物的存在或不存在： 存在或不存在可检测特性。 微粒在测试混合物中的相对量，其有效加速在测试样品和测试混合物的混合物内的微生物生物膜的形成。

公开(公告)号：US08268579B2

公开(公告)日：2012-09-18

申请号：US12356847

申请日：2009-01-21

申请人： Stephen C. Edberg

发明人： Stephen C. Edberg

IPC分类号： C12Q1/04

CPC分类号： C12Q1/14

摘要： A presence/absence test for Staphylococcus aureus (S. aureus) involves placing a first generation test sample in a solution that will clot in the presence of S. aureus. The solution contains components that will selectively grow S. aureus and also contains clotting factors that will react with S. aureus, if S. aureus is present in the sample, to clot the solution. Examples of specimen samples that can be tested include nasal swabs and lesion swabs, among others. The test can also be modified to detect the presence or absence of methicillin resistant S. Aureus (MRSA).

摘要翻译： 金黄色葡萄球菌（金黄色葡萄球菌）的存在/不存在测试涉及将第一代测试样品置于在金黄色葡萄球菌存在下凝结的溶液中。 该溶液含有将选择性生长金黄色葡萄球菌的成分，并且还含有与金黄色葡萄球菌反应的凝血因子，如果样品中存在金黄色葡萄球菌以凝结溶液。 可以测试的标本样品的实例包括鼻拭子和病变拭子等。 也可以修改测试以检测耐甲氧西林金黄色葡萄球菌（MRSA）的存在或不存在。

公开(公告)号：US20120149056A1

公开(公告)日：2012-06-14

申请号：US12823698

申请日：2010-06-25

申请人： Stephen C. Edberg

发明人： Stephen C. Edberg

IPC分类号： C12Q1/04

CPC分类号： C12Q1/045 ,  C12Q1/04 ,  C12Q1/10 ,  C12Q1/14 ,  G01N2333/415

摘要： Reagent mixtures and testing procedures for detecting the presence or absence of various target substances such as aureus; vancomycin-resistant enterrococcus; Group B Streptococcus; E. coli; fungi; and others, involve the use of a testing reagents which include growth factors for the particular target substance, a detectable signal generator which is sensitive to the presence of the target substance, and hatmonal compounds which will accelerate the growth of the target substance within a specimen being tested. Examples of specimen samples that can be tested include nasal swabs, throat swabs, lesion swabs, environmental swabs, foodstuffs, and water, to mention a few.

摘要翻译： 用于检测各种目标物质如金黄色葡萄球菌存在或不存在的试剂混合物和检测程序; 耐万古霉素肠球菌; B组链球菌 大肠杆菌; 菌类; 涉及使用包括特定目标物质的生长因子的测试试剂，对目标物质的存在敏感的可检测信号发生器，以及加速样品中目标物质生长的hat al化合物 被测试。 可以测试的标本样品的实例包括鼻拭子，咽拭子，病变拭子，环境拭子，食品和水等等。

公开(公告)号：US20140349325A1

公开(公告)日：2014-11-27

申请号：US14357489

申请日：2011-11-10

申请人： Stephen C. Edberg

发明人： Stephen C. Edberg

IPC分类号： C12Q1/14

CPC分类号： C12Q1/14 ,  C12Q1/56 ,  G01N2333/31 ,  G01N2333/952

摘要： A method and medium for detecting the presence or absence of a targeted strain of antibiotic resistant pathogenic staphylococci in a test sample is provided. The method includes the steps of: a) providing a test medium having at least one hydrolysable substrate, which substrate is operable to promote the growth of the targeted strain of antibiotic resistant pathogenic staphylococci and to produce a detectable signal when the hydrolyzable substrate is metabolized by the targeted strain of antibiotic resistant pathogenic staphylococci; b) forming a mixture of the test sample and hydrated test medium in the test tube; c) incubating the mixture at temperatures in the range of about 20° C. to about 35° C.; and d) detecting the presence or absence of the targeted strain of antibiotic resistant pathogenic staphylococci in the test sample by the presence or absence of the detectable signal in the mixture in the tube.

摘要翻译： 提供了一种用于检测试验样品中抗生素抗性致病性葡萄球菌目标菌株存在或不存在的方法和培养基。 该方法包括以下步骤：a）提供具有至少一个可水解底物的测试介质，该底物可操作以促进抗生素抗性致病性葡萄球菌的靶向菌株的生长，并且当可水解底物代谢时产生可检测信号 抗生素抗性致病性葡萄球菌的靶向菌株; b）在试管中形成试验样品和水合试验介质的混合物; c）在约20℃至约35℃的温度范围内孵育该混合物; 和d）通过管中混合物中存在或不存在可检测信号，检测测试样品中抗生素抗性致病性葡萄球菌目标菌株的存在或不存在。

公开(公告)号：US08765395B2

公开(公告)日：2014-07-01

申请号：US13181157

申请日：2011-07-12

申请人： Stephen C. Edberg

发明人： Stephen C. Edberg

IPC分类号： C12Q1/14

CPC分类号： C12Q1/14

摘要： A presence/absence test for Staphylococcus aureus (S. aureus) involves placing a first generation test sample in a solution that will clot in the presence of S. aureus. The solution contains components that will selectively grow S. aureus and also contains clotting factors that will react with S. aureus, if S. aureus is present in the sample, to clot the solution. Examples of specimen samples that can be tested include nasal swabs and lesion swabs, among others. The test can also be modified to detect the presence or absence of methicillin resistant S. Aureus (MRSA).

摘要翻译： 金黄色葡萄球菌（金黄色葡萄球菌）的存在/不存在测试涉及将第一代测试样品置于在金黄色葡萄球菌存在下凝结的溶液中。 该溶液含有将选择性生长金黄色葡萄球菌的成分，并且还含有与金黄色葡萄球菌反应的凝血因子，如果样品中存在金黄色葡萄球菌以凝结溶液。 可以测试的标本样品的实例包括鼻拭子和病变拭子等。 也可以修改测试以检测耐甲氧西林金黄色葡萄球菌（MRSA）的存在或不存在。

公开(公告)号：US20110027823A1

公开(公告)日：2011-02-03

申请号：US12823665

申请日：2010-06-25

申请人： Stephen C. Edberg

发明人： Stephen C. Edberg

IPC分类号： C12Q1/14

CPC分类号： C12Q1/14

摘要： A presence/absence test for Staphylococcus aureus (S. aureus) involves placing a first generation test sample in a solution that will clot in the presence of S. aureus. The solution contains components that will selectively grow S. aureus and also contains clotting factors that will react with S. aureus, if S. aureus is present in the sample, to clot the solution. Examples of specimen samples that can be tested include nasal swabs and lesion swabs, among others. The test can also be modified to detect the presence or absence of methicillin resistant S. Aureus (MRSA). The addition of micro particles having a size in the range of about 0.1 micron to about 1.0 mm provides localities where the bacteria agglomerate, thereby significantly decreasing the clotting time, and providing a significantly stronger clot. The micro particles can be used in other bacteria tests to accelerate the production of an end result. Such other tests can include a vancomycin-resistant enterococcus test; a Group B Streptococcus test; a test for hemolytic E. coli; and a test for Listeria monocytogenes, to name a few. These tests are all performed in a liquid broth-type reagent mixture and do not necessarily involve clotting of the broth.

摘要翻译： 金黄色葡萄球菌（金黄色葡萄球菌）的存在/不存在测试涉及将第一代测试样品置于在金黄色葡萄球菌存在下凝结的溶液中。 该溶液含有将选择性生长金黄色葡萄球菌的成分，并且还含有与金黄色葡萄球菌反应的凝血因子，如果样品中存在金黄色葡萄球菌以凝结溶液。 可以测试的标本样品的实例包括鼻拭子和病变拭子等。 也可以修改测试以检测耐甲氧西林金黄色葡萄球菌（MRSA）的存在或不存在。 加入尺寸在约0.1微米至约1.0毫米范围内的微粒提供了细菌聚集的地方，从而显着降低凝固时间，并提供明显更强的凝块。 微粒可用于其他细菌测试，以加速最终结果的产生。 这样的其他测试可以包括耐万古霉素肠球菌测试; B组链球菌试验; 溶血性大肠杆菌检测; 和单核细胞增多性李斯特氏菌的检测，仅举几例。 这些测试都是在液体肉汤型试剂混合物中进行的，并不一定涉及肉汤的凝结。

公开(公告)号：US08846336B2

公开(公告)日：2014-09-30

申请号：US13943400

申请日：2013-07-16

申请人： Pilots Point LLC

发明人： Stephen C. Edberg

IPC分类号： C12Q1/04

CPC分类号： C12Q1/06 ,  C12Q1/04 ,  C12Q1/045 ,  C12Q1/10 ,  C12Q1/14 ,  C12Q1/34 ,  C12Q2304/00 ,  G01N21/78

摘要： A method of and test mixture for detecting the presence or absence of a target microbe in a sample is provided. The method includes the steps of: a) providing a test mixture that includes micro particles, a substrate in an amount that is sufficient to support log phase growth of the target microbe until a detectable characteristic signal is produced in the test mixture and sample admixture, and an amount of vitamin, amino acid, element and salt ingredients; b) combining the powdered test mixture and sample to form the admixture; and c) detecting the presence or absence of target microbes in the sample based on the presence or absence of the detectable characteristic.

摘要翻译： 提供了用于检测样品中存在或不存在目标微生物的混合物的方法和测试方法。 该方法包括以下步骤：a）提供测试混合物，其包括微粒，底物的量足以支持目标微生物的对数生长，直到在测试混合物和样品混合物中产生可检测的特征信号， 和一定量的维生素，氨基酸，元素和盐成分; b）将粉末状测试混合物和样品混合以形成混合物; 和c）基于可检测特征的存在或不存在来检测样品中目标微生物的存在或不存在。