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    LIGAND-TARGETED CELL CONJUGATE (LTCC)-BASED ANTI-TUMOR IMMUNE CELL, AS WELL AS PREPARATION METHOD AND USE THEREOF
    4.
    发明申请
    LIGAND-TARGETED CELL CONJUGATE (LTCC)-BASED ANTI-TUMOR IMMUNE CELL, AS WELL AS PREPARATION METHOD AND USE THEREOF 有权

    公开(公告)号：US20220202949A1

    公开(公告)日：2022-06-30

    申请号：US17551412

    申请日：2021-12-15

    Applicant: AFFILIATED HOSPITAL OF JIANGNAN UNIVERSITY ,  JIANGNAN UNIVERSITY

    Inventor： Zhaohui HUANG ,  Zhimeng WU ,  Liang GONG ,  Yanchun LI

    IPC: A61K47/69 ,  C12N5/0783 ,  A61K47/68 ,  A61P35/00

    Abstract: Non-natural sugars modified with a bioorthogonal reactive group are added into a culture medium of immune cells such as NK cells to obtain immune cells modified with the bioorthogonal reactive group; and then, under a physiological condition, a targeting ligand, for example, a nanobody, is modified to a surface of each of the immune cells through a bioorthogonal reaction, wherein the targeting ligand has one terminal with a bioorthogonal reactive pairing group which is capable of being matched and connected with the bioorthogonal reactive group to generate a connecting reaction, connection is implemented by a transpeptidase SrtA-mediated chemoenzymatic method. The targeting ligand highly specifically recognizes and binds to a highly expressed receptor on the surface of tumor cells. The immune cell modified with the targeting ligand can specifically bind in a targeted way to the tumor cells, therefore generating cytokines, killing and damaging tumor cells.

    Nicotinamide dummy template surface molecularly imprinted polymer and preparation method and application thereof
    5.
    发明授权
    Nicotinamide dummy template surface molecularly imprinted polymer and preparation method and application thereof 有权

    公开(公告)号：US12214329B2

    公开(公告)日：2025-02-04

    申请号：US17551338

    申请日：2021-12-15

    Applicant: Jiangnan University

    Inventor： Hongxin Wang ,  Jingyu Chen ,  Shijia Wu ,  Xinlin Wei ,  Xin Huang ,  Li Wang

    IPC: B01J20/26 ,  A23F3/20 ,  B01D15/22 ,  B01J20/30 ,  C07C37/82 ,  C07C231/24 ,  C08F292/00

    Abstract: The disclosure discloses a nicotinamide dummy template surface molecularly imprinted polymer, a preparation method and application thereof, and belongs to the technical field of chemical materials. The preparation method of the disclosure includes the steps of preparing a modified silica gel carrier, preparing a dummy template surface molecularly imprinted polymer and the like. The disclosure uses nicotinamide, a structural analogue of imidacloprid and acetamiprid, as a dummy template to prepare a silica gel surface molecularly imprinted polymer. The polymer not only can effectively avoid pollution caused by the leakage of template molecules, but also can specifically remove imidacloprid and acetamiprid from water-soluble tea extracts. The removal rate of imidacloprid and acetamiprid is greater than 96% and 93%, respectively, and the loss of tea polyphenols in the extracts is less than 10%. In addition, the molecularly imprinted adsorption column prepared by the disclosure can be eluted with ethanol solution, and the eluted adsorption column can be recycled, so the disclosure can be well applied to the preparation technology of tea extracts and has good application prospects.

    Saccharomyces Cerevisiae Lysate Containing One or More Cytokines, Preparation Method Therefor and Application Thereof in Cell-Cultured Meat
    6.
    发明申请
    Saccharomyces Cerevisiae Lysate Containing One or More Cytokines, Preparation Method Therefor and Application Thereof in Cell-Cultured Meat 有权

    公开(公告)号：US20250019735A1

    公开(公告)日：2025-01-16

    申请号：US18898838

    申请日：2024-09-27

    Applicant: Jiangnan University

    Inventor： Xin Guan ,  Jingwen Zhou ,  Qingzi Lei ,  Jian Chen ,  Guocheng Du

    IPC: C12P21/02 ,  C07K14/485 ,  C07K14/49 ,  C07K14/50 ,  C07K14/65

    Abstract: The disclosure discloses a Saccharomyces cerevisiae lysate containing one or more cytokines, a preparation method therefor and an application thereof in cell-cultured meat, and belongs to the technical field of genetic engineering and cell-cultured meat. The disclosure provides a method for further increasing recombinant cytokine yields using the GRAS strain of S. cerevisiae recombinantly expressing a single cytokine or co-expressing a combination of cytokines through promoter optimization, knockout of endogenous protease in yeast, genome-integrated expression and other means. The yeast lysate may be directly used for muscle stem cell culture after the process such as filtration, sterilization, and cytokine concentration measurement, effectively promoting muscle stem cell proliferation. The disclosure avoids the complex purification process in the production of recombinant cytokines, reduces the cost of cytokine production, and provides new ideas for large-scale low-cost development of cell-cultured meat.

    Direct ink writing three-dimensional printing method based on near-infrared photopolymerization
    7.
    发明授权
    Direct ink writing three-dimensional printing method based on near-infrared photopolymerization 有权

    公开(公告)号：US12194672B2

    公开(公告)日：2025-01-14

    申请号：US17603676

    申请日：2020-07-09

    Applicant: JIANGNAN UNIVERSITY

    Inventor： Ren Liu ,  Yu Liu ,  Junzhe Zhu

    IPC: B29C64/129 ,  B29C64/106 ,  B29C64/209 ,  B29C64/264 ,  C08F2/46 ,  C08F222/10 ,  C09D11/101 ,  C09D11/107 ,  B33Y10/00 ,  B33Y30/00 ,  B33Y70/00 ,  B33Y80/00

    Abstract: The invention relates to an ink based on near-infrared light polymerization. The method and technology of direct writing three-dimensional printing belong to the field of material processing technology area. The method is: direct writing nozzles move in three-dimensional space or stationery, the ink is squeezed out of the direct writing nozzle, receiving the near-infrared light irradiation, after curing, complete the three-dimensional object forming and curing. The solidifying time t does not exceed the ratio of near-infrared light diameter dl and the ink extrusion speed vi, that is, t≤dl/vi. Since near-infrared light has a better medium mass penetration, can penetrate the structure during molding to promote both internal and external to a higher degree of curing, so as to achieve cross-scale structure 3D printing, and the method provided by the present invention accurately controls solidifying process of the ink and therefore achieve the DIW array 3D structure real-time curing.

    Method for Improving Acetate Tolerance and Lipid Accumulation of Oleaginous Microorganism Using Acetyl-CoA Synthetase (ACS)
    8.
    发明申请
    Method for Improving Acetate Tolerance and Lipid Accumulation of Oleaginous Microorganism Using Acetyl-CoA Synthetase (ACS) 有权

    公开(公告)号：US20250002893A1

    公开(公告)日：2025-01-02

    申请号：US18830762

    申请日：2024-09-11

    Applicant: Jiangnan University

    Inventor： Haiqin Chen ,  Lulu Chang ,  Bo Yang ,  Jianxin Zhao ,  Wei Chen

    IPC: C12N9/00 ,  C12N9/04 ,  C12N9/12 ,  C12N9/88 ,  C12N15/80 ,  C12P7/64

    Abstract: The present disclosure discloses a method for improving acetate tolerance and lipid accumulation of an oleaginous microorganism using an acetyl-CoA synthetase (ACS) and belongs to the technical fields of genetic engineering and microbial engineering. The present disclosure overexpresses endogenous constitutive MaACS in Mortierella alpina, improves the acetate tolerance and lipid yield of the recombinant strain, and the proportion of polyunsaturated fatty acids, and further improves the lipid accumulation using a culture medium taking ammonium acetate as a nitrogen source. The present expresses a citrate lyase ACL1 while overexpressing the MaACS in a combined manner, or expresses glucose-6-phosphate dehydrogenase G6PD2 to equilibrate the supply of reducing power NADPH so as to avoid the overflow of the acetyl-CoA, or knocks down a γ subunit SNF1 γ of a sucrose non-fermenting-related protein kinase which negatively regulates lipid accumulation to improve the activity of an acetyl-CoA carboxylase 1.

    Oil Crystallization Promoter, Preparation Method Therefor and Use Thereof
    9.
    发明申请
    Oil Crystallization Promoter, Preparation Method Therefor and Use Thereof 有权

    公开(公告)号：US20240407384A1

    公开(公告)日：2024-12-12

    申请号：US18811929

    申请日：2024-08-22

    Applicant: Jiangnan University

    Inventor： Yuanfa LIU ,  Xiuhang CHAI

    IPC: A23D9/007 ,  A23D9/04 ,  C11B7/00

    Abstract: Disclosed are an oil crystallization promoter, a preparation method therefor and a use thereof, belonging to the technical field of dietary oil processing and control. Components of the oil crystallization promoter of the present disclosure include, by mass percentage: 50%-70% Dipalmito-olein (PO), 10%-20% Dipalmitin (PP), and 15%-30% Diolein (OO), adding up to 100%. The oil crystallization promoter of the present disclosure can increase the crystallization rate of oil, promote rapid formation and stabilization of a stable β′ crystal form of the oil, accelerate rapid crystallization and stabilization of confectionery- and baking-specific fat products such as margarine, shortening and cocoa butter substitute, and inhibit product graininess and post-hardening.

    Nucleic acid aptamer specifically recognizing β-lactoglobulin and use thereof
    10.
    发明授权
    Nucleic acid aptamer specifically recognizing β-lactoglobulin and use thereof 有权

    公开(公告)号：US12152245B2

    公开(公告)日：2024-11-26

    申请号：US17416490

    申请日：2020-06-24

    Applicant: JIANGNAN UNIVERSITY

    Inventor： Nuo Duan ,  Shijia Wu ,  Shuo Qi ,  Zhouping Wang

    IPC: C12N15/115 ,  G01N33/68

    Abstract: The present invention provides a nucleic acid aptamer specifically recognizing β-lactoglobulin and use thereof. The nucleic acid aptamer has a sequence as shown in SEQ ID NO:1, a sequence having 60% or higher homology to the sequence as shown in SEQ ID NO:1 and specifically recognizing β-lactoglobulin, or a sequence derived from the sequence as shown in SEQ ID NO:1 and specifically recognizing β-lactoglobulin. The nucleic acid aptamer specifically binds to the allergen β-lactoglobulin in cow milk and dairy products, thereby providing a new tool for the high-sensitivity and low-cost detection of the allergen β-lactoglobulin.

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