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    Cutinase cleaning compositions
    1.
    发明授权
    Cutinase cleaning compositions 失效
    角质酶清洗组合物

    公开(公告)号：US4981611A

    公开(公告)日：1991-01-01

    申请号：US341200

    申请日：1989-03-29

    Applicant: Pappachan Kolattukudy ,  Ayrookaran J. Poulose

    Inventor： Pappachan Kolattukudy ,  Ayrookaran J. Poulose

    IPC: C11D3/386 ,  C12N9/18

    CPC classification number: C11D3/38636 ,  C12N9/18

    Abstract: This invention relates to cleaning compositions and methods for using them. Particularly, the invention relates to compositions comprising a surfactant and a cutinase enzyme. A preferred cutinase is derived from Pseudomonas putida ATCC 53552. Excellent cleaning is obtained with a surfactant mixture containing sodium dodecyl sulfate and octoxynol.

    Abstract translation: PCT No.PCT / US88 / 01844 Sec。 371日期：1989年3月29日 102（e）日期1989年3月29日PCT提交1988年5月31日PCT公布。 出版物WO88 / 09367 日本公开日1988年12月1日。本发明涉及清洁组合物及其使用方法。 特别地，本发明涉及包含表面活性剂和角质酶的组合物。 优选的角质酶衍生自恶臭假单胞菌ATCC 53552。用含十二烷基硫酸钠和辛苯甲醇的表面活性剂混合物获得极好的清洗。

    Subtilisin modifications to enhance oxidative stability
    2.
    发明授权
    Subtilisin modifications to enhance oxidative stability 失效
    枯草杆菌蛋白酶修饰以增强氧化稳定性

    公开(公告)号：US5346823A

    公开(公告)日：1994-09-13

    申请号：US36592

    申请日：1993-03-24

    Applicant: David A. Estell ,  James A. Wells ,  Richard R. Bott

    Inventor： David A. Estell ,  James A. Wells ,  Richard R. Bott

    IPC: C11D3/386 ,  C12N9/54 ,  C12N15/10 ,  C12N15/75 ,  C12N15/00 ,  C12N15/11 ,  C12N15/57

    CPC classification number: C12R1/07 ,  C11D3/386 ,  C12N15/102 ,  C12N15/75 ,  C12N9/54

    Abstract: There are provided methods for making a mutant Bacillus subtilisin having altered oxidative stability, the methods comprising obtaining DNA fragment consisting of a region coding for a Bacillus subtilisin, and introducing a mutation into said DNA fragment such that the mutation is introduced in a region encoding a methionine, tryptophan, cysteine or lysine, sensitive to oxidation, such that upon expression of the mutant subtilisin one or more codon regions encoding for methionine, tryptophan, cysteine or lysine is replaced with an amino acid other than methionine, tryptophan, cysteine or lysine, preferably alanine or serine.

    Abstract translation: 提供了制备具有改变的氧化稳定性的突变型枯草杆菌蛋白酶的方法，所述方法包括获得由编码枯草杆菌蛋白酶的区域组成的DNA片段，并将突变引入所述DNA片段，使得突变引入到编码 敏感的蛋氨酸，色氨酸，半胱氨酸或赖氨酸，使得突变型枯草杆菌蛋白酶表达后，编码甲硫氨酸，色氨酸，半胱氨酸或赖氨酸的一个或多个密码子区域被甲硫氨酸，色氨酸，半胱氨酸或赖氨酸以外的氨基酸替代， 优选丙氨酸或丝氨酸。

    Use of cellulase in a method of wet milling a starch-containing grain
    4.
    发明授权
    Use of cellulase in a method of wet milling a starch-containing grain 失效
    在湿法研磨含淀粉颗粒的方法中使用纤维素酶

    公开(公告)号：US4795101A

    公开(公告)日：1989-01-03

    申请号：US49750

    申请日：1987-05-13

    Applicant: Scott C. Silver

    Inventor： Scott C. Silver

    IPC: C08B30/04

    CPC classification number: C08B30/044

    Abstract: The invention relates to a method of milling grain, especially corn, comprising cleaning the grain, steeping the grain in water to soften it, and then milling the grain with a cellulase enzyme.

    Abstract translation: 本发明涉及一种研磨谷物，特别是玉米的方法，包括清洗谷物，将谷物浸泡在水中软化，然后用纤维素酶研磨谷物。

    Method for protein removal from tobacco
    6.
    发明授权
    Method for protein removal from tobacco 失效
    从烟草中去除蛋白质的方法

    公开(公告)号：US4716911A

    公开(公告)日：1988-01-05

    申请号：US849575

    申请日：1986-04-08

    Applicant: A. J. Poulose ,  Stanley E. Mainzer

    Inventor： A. J. Poulose ,  Stanley E. Mainzer

    IPC: A24B15/20 ,  A24B15/28

    CPC classification number: A24B15/28 ,  A24B15/20

    Abstract: An improved process for removing insoluble nitrogen-containing compounds from cured tobacco uses alkali or a combination of protease and nonprotease depolymerase, rather than simple protease extraction.The method of the invention is more efficient and results in a more effective extraction of protein.

    Abstract translation: 用于从固化烟草中除去不溶性含氮化合物的改进方法使用碱或蛋白酶和非蛋白酶解聚酶的组合，而不是简单的蛋白酶提取。 本发明的方法更有效并且导致蛋白质的更有效的提取。

    Process for producing heterologous polypeptides
    8.
    发明授权
    Process for producing heterologous polypeptides 失效
    用于产生异源多肽的方法

    公开(公告)号：US5411873A

    公开(公告)日：1995-05-02

    申请号：US928697

    申请日：1992-08-11

    Applicant: Robin M. Adams ,  Scott D. Power ,  David B. Powers ,  James A. Wells ,  Daniel G. Yansura

    Inventor： Robin M. Adams ,  Scott D. Power ,  David B. Powers ,  James A. Wells ,  Daniel G. Yansura

    IPC: C11D3/386 ,  C12N9/54 ,  C12N15/10 ,  C12N15/52 ,  C12N15/75 ,  C12P21/02

    CPC classification number: C12N15/52 ,  C11D3/386 ,  C12N15/102 ,  C12N15/75 ,  C12N9/54 ,  C12R1/07

    Abstract: Processes for producing various heterologous polypeptides which when expressed are either incorrectly processed and hence asssociated with the surface of the host cell or are not processed to mature form. More specifically, processes for the production of heterologous non-human carbonyl hydrolases expressed either in host cells incapable of producing enzymatically active endoprotease or host cells deficient in enzymatically active extracellular endoprotease are disclosed. Such non-human carbonyl hydrolases generally are incapable of autoproteolytic maturation and become associated with the surface of expression hosts which are deficient in enzymatically active extracellular endoprotease. Processes for preparing non-human carbonyl hydrolase and heterologous polypeptides which are expressed as part of a fusion polypeptide are also disclosed, as well as non-human carbonyl hydrolases which are substantially free of the host cell membrane with which they are normally associated.

    Abstract translation: 用于产生各种异源多肽的方法，当被表达时，它们被错误地加工并因此与宿主细胞的表面相结合或者不被加工成成熟形式。 更具体地，公开了在不能产生酶促活性内切蛋白酶的宿主细胞中表达的异源非人羰基水解酶的生产方法或在酶活性细胞外内蛋白酶中缺乏的宿主细胞。 这种非人羰基水解酶通常不能自身蛋白水解成熟，并且与酶活性细胞外内蛋白酶缺陷的表达宿主的表面相关联。 还公开了制备非人羰基水解酶和作为融合多肽的一部分表达的异源多肽的方法，以及基本上不含与它们通常结合的宿主细胞膜的非人羰基水解酶。

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