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    Composition for preventing, alleviating, or treating arthritis or osteoporosis, containing neoagarooligosaccharide
    1.
    发明授权
    Composition for preventing, alleviating, or treating arthritis or osteoporosis, containing neoagarooligosaccharide 有权

    公开(公告)号：US10933086B2

    公开(公告)日：2021-03-02

    申请号：US16345082

    申请日：2017-10-17

    申请人： DYNEBIO INC.

    发明人： Je Hyeon Lee ,  Eun Joo Kim ,  Kyoung Woon Kim

    IPC分类号： A61K31/729 ,  A23L29/30 ,  A23L33/125 ,  A61P19/02 ,  A61K31/702 ,  C12P19/04 ,  A23L33/10

    摘要： Provided in the present invention is a novel use of composition containing a neoagarooligosaccharide mixture as an active ingredient. The neoagarooligosaccharide mixture and the like as an active ingredient of the composition according to the present invention can reduce the expression of RANKL, an osteoclast inducer, or IL-6, an inflammatory cytokine, and effectively inhibit differentiation of monocytes into osteoclasts. Thus, the composition according to the present invention may be used as a medicine or functional food for preventing, alleviating, or treating arthritis (especially rheumatoid arthritis). Further, the active ingredient of the composition according to the present invention can be obtained by an enzyme reaction between a readily available substrate such as agar or agarose and DagA as a beta-agarase derived from Streptomyces coelicolor. Thus, it can be produced at a relatively low cost and can easily be ingested by anyone because it has no side effects.

    Streptomyces coelicolor mutant strain, method of producing β-agarase by using same, and method of producing neoagaro-oligosaccharides by using same
    2.
    发明授权
    Streptomyces coelicolor mutant strain, method of producing β-agarase by using same, and method of producing neoagaro-oligosaccharides by using same 有权

    公开(公告)号：US11466248B2

    公开(公告)日：2022-10-11

    申请号：US16980042

    申请日：2019-09-10

    申请人： DYNEBIO INC.

    发明人： Je Hyeon Lee ,  Eun Joo Kim ,  Yeon Hee Lee

    IPC分类号： C12N1/20 ,  C12P19/04 ,  C12P19/14

    摘要： The present invention provides Streptomyces coelicolor strain A3(2)_M22-2C43 obtained by inducing a point mutation in the base sequence of the DagB gene in a wild-type Streptomyces coelicolor strain A3(2) by UV radiation. Since the Streptomyces coelicolor strain A3(2)_M22-2C43 according to the present invention expresses a DagB mutant enzyme expressing little or no DagB beta-agarase or exhibiting little or no beta-agarase activity, there is no need for separate isolation and purification of DagA enzymes from culture fluid, and the culture fluid of the Streptomyces coelicolor strain A3(2)_M22-2C43 or supernatant thereof may be used to produce, from agar or agarose, neoagarose oligosaccharides with a higher content of neoagarotetraose or neoagarohexaose than that of neoagarobiose.

    STREPTOMYCES COELICOLOR MUTANT STRAIN, METHOD OF PRODUCING BETA-AGARASE BY USING SAME, AND METHOD OF PRODUCING NEOAGARO-OLIGOSACCHARIDES BY USING SAME
    4.
    发明申请
    STREPTOMYCES COELICOLOR MUTANT STRAIN, METHOD OF PRODUCING BETA-AGARASE BY USING SAME, AND METHOD OF PRODUCING NEOAGARO-OLIGOSACCHARIDES BY USING SAME 有权

    公开(公告)号：US20210009940A1

    公开(公告)日：2021-01-14

    申请号：US16980042

    申请日：2019-09-10

    申请人： DYNEBIO INC.

    发明人： Je Hyeon LEE ,  Eun Joo KIM ,  Yeon Hee LEE

    IPC分类号： C12N1/20 ,  C12P19/04 ,  C12P19/14

    摘要： The present invention provides Streptomyces coelicolor strain A3(2)_M22-2C43 obtained by inducing a point mutation in the base sequence of the DagB gene in a wild-type Streptomyces coelicolor strain A3(2) by UV radiation. Since the Streptomyces coelicolor strain A3(2)_M22-2C43 according to the present invention expresses a DagB mutant enzyme expressing little or no DagB beta-agarase or exhibiting little or no beta-agarase activity, there is no need for separate isolation and purification of DagA enzymes from culture fluid, and the culture fluid of the Streptomyces coelicolor strain A3(2)_M22-2C43 or supernatant thereof may be used to produce, from agar or agarose, neoagarose oligosaccharides with a higher content of neoagarotetraose or neoagarohexaose than that of neoagarobiose.