公开(公告)号：US11557072B2

公开(公告)日：2023-01-17

申请号：US17432094

申请日：2021-05-19

申请人： AFFILIATED HOSPITAL OF JIANGSU UNIVERSITY

发明人： Zhongqun Wang ,  Yue Geng ,  Lihua Li ,  Chen Shao ,  Zhen Sun ,  Guangyao Zang ,  Lili Zhang

IPC分类号： G06T11/00 ,  G06V10/25 ,  G06V10/46 ,  G06V10/75 ,  G06V10/50 ,  G06V10/22 ,  G06V10/36 ,  A61B6/03 ,  A61B6/00

摘要： The present invention discloses a clustering algorithm-based multi-parameter cumulative calculation method for lower limb vascular calcification indexes, including the following steps: firstly carrying out super-pixel segmentation of a CT image, and enabling calcified spots in the CT image to be segmented in each super-pixel region; after the super-pixel segmentation is accomplished, extracting a brightness characteristic value of a super-pixel region where the calcified spots are located by using a Lab color space, and performing edge detection and contour extraction on the calcified spots in the image; and after edge detection and contour extraction, fitting the calcified spots in the image by using a segmented ellipse, and extracting the area of the calcified spots after optimizing an ellipse contour.

公开(公告)号：US20220148237A1

公开(公告)日：2022-05-12

申请号：US17432094

申请日：2021-05-19

申请人： AFFILIATED HOSPITAL OF JIANGSU UNIVERSITY

发明人： Zhongqun WANG ,  Yue GENG ,  Lihua LI ,  Chen SHAO ,  Zhen SUN ,  Guangyao ZANG ,  Lili ZHANG

IPC分类号： G06T11/00 ,  G06V10/25 ,  G06V10/46 ,  G06V10/36 ,  G06V10/50 ,  G06V10/22 ,  G06V10/75 ,  A61B6/03 ,  A61B6/00

摘要： The present invention discloses a clustering algorithm-based multi-parameter cumulative calculation method for lower limb vascular calcification indexes, including the following steps: firstly carrying out super-pixel segmentation of a CT image, and enabling calcified spots in the CT image to be segmented in each super-pixel region; after the super-pixel segmentation is accomplished, extracting a brightness characteristic value of a super-pixel region where the calcified spots are located by using a Lab color space, and performing edge detection and contour extraction on the calcified spots in the image; and after edge detection and contour extraction, fitting the calcified spots in the image by using a segmented ellipse, and extracting the area of the calcified spots after optimizing an ellipse contour.

公开(公告)号：US20240336894A1

公开(公告)日：2024-10-10

申请号：US18402999

申请日：2024-01-03

申请人： Affiliated Hospital of Jiangsu University

发明人： Xuefeng Wang ,  Yongbin Ma

IPC分类号： C12N5/0786

CPC分类号： C12N5/0645 ,  C12N2500/02 ,  C12N2500/05 ,  C12N2500/30 ,  C12N2500/84 ,  C12N2509/00 ,  C12N2523/00 ,  C12N2527/00 ,  C12N2529/00

摘要： A simple and efficient method for separating migrasomes from macrophages is provided. The method separates migrasomes having a diameter range of 0.5 micrometers (μm) to 3 μm by intercepting through a filter and eluting through reverse filtration successfully. The separated migrasome has a vesicle-liked structure and wrinkles on its surface, and the separated migrasome has a diameter over 500 nanometers (nm). The separated migrasomes express their characteristic proteins PIGK, EOGT, and TSPAN4, but do not express specific markers TSG101 and ALIX of EVs, indicating that the separated migrasomes are a unique type of vesicles distinct from extracellular vesicles (EVs). The integrity of ribonucleic acids (RNA) carried by the migrasomes is not affected. The method for separating migrasomes from macrophages has the characteristics of simplicity, high efficiency, good controllability, good repeatability, and low cost, and large special equipment is not needed.