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公开(公告)号：US20110111426A1
公开(公告)日：2011-05-12
申请号：US13000029
申请日：2008-06-27
IPC分类号： G01N33/53
CPC分类号： A61K36/48 , G01N33/566 , G01N2333/70571 , G01N2500/02
摘要： The invention intends to find out a bioassay system with an in-vitro test capable of ensuring the higher quality of yokukansan, and provides a bioassay method for yokukansan, comprising competitively reacting a labeled ligand and yokukansan with cells or cell membranes expressing glutamate receptors, measuring the binding activity of yokukansan, and evaluating the pharmacological activity value of yokukansan from the measurement value.
摘要翻译： 本发明旨在找出具有能够确保更高质量的洋甘康素的体外试验的生物测定系统，并且提供了一种用于洋葱素的生物测定方法，其包括使标记的配体和Yokukansan与表达谷氨酸受体的细胞或细胞膜竞争性地反应，测量 yokukansan的结合活性，并从测定值评价yokukansan的药理活性值。
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公开(公告)号：US08268578B2
公开(公告)日：2012-09-18
申请号：US12668519
申请日：2008-06-27
申请人： Yasushi Ikarashi , Zenji Kawakami
发明人： Yasushi Ikarashi , Zenji Kawakami
CPC分类号： G01N33/5058
摘要： To find out an in-vitro bioassay system capable of ensuring qualities of yokukansan to a higher degree, it is intended to provide a method of bioassaying yokukansan characterized by comprising adding a yokukansan-containing test sample to astroglial cells to be cultivated under the condition of thiamine deficiency, and then determining the pharmacological activity value of yokukansan based on the glutamic acid or neutral red intake level in the cultivated astroglial cells.
摘要翻译： 为了找出能够更高程度地确保洋葱素质量的体外生物测定系统，其目的是提供一种生物测定yokukansan的方法，其特征在于包括将含有Yokukansan的测试样品添加到待培养的星形胶质细胞中， 硫胺素缺乏症，然后根据培养的星形胶质细胞中的谷氨酸或中性红摄取水平确定洋葱素的药理活性值。
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公开(公告)号：US20100196944A1
公开(公告)日：2010-08-05
申请号：US12668519
申请日：2008-06-27
申请人： Yasushi Ikarashi , Zenji Kawakami
发明人： Yasushi Ikarashi , Zenji Kawakami
IPC分类号： C12Q1/02
CPC分类号： G01N33/5058
摘要： To find out an in-vitro bioassay system capable of ensuring qualities of yokukansan to a higher degree, it is intended to provide a method of bioassaying yokukansan characterized by comprising adding a yokukansan-containing test sample to astroglial cells to be cultivated under the condition of thiamine deficiency, and then determining the pharmacological activity value of yokukansan based on the glutamic acid or neutral red intake level in the cultivated astroglial cells.
摘要翻译： 为了找出能够更高程度地确保洋葱素质量的体外生物测定系统，其目的是提供一种生物测定yokukansan的方法，其特征在于包括将含有Yokukansan的测试样品添加到待培养的星形胶质细胞中， 硫胺素缺乏症，然后根据培养的星形胶质细胞中的谷氨酸或中性红摄取水平确定洋葱素的药理活性值。
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公开(公告)号：US08497092B2
公开(公告)日：2013-07-30
申请号：US12936282
申请日：2008-04-03
申请人： Yasushi Ikarashi , Zenji Kawakami
发明人： Yasushi Ikarashi , Zenji Kawakami
IPC分类号： C12P1/00
CPC分类号： A61K36/18 , G01N33/5014 , G01N33/5058
摘要： The invention intends to find out a bioassay system with an in-vitro test capable of ensuring the higher quality of yokukansan, and provides a bioassay method for yokukansan, comprising adding glutamate in an amount sufficient to induce cell death and yokukansan to a medium for culturing cells, and evaluating pharmacological activity value of yokukansan from viability of the cultured cells in the medium.
摘要翻译： 本发明旨在找出具有能够确保更高品质的洋甘康素的体外试验的生物测定系统，并且提供了对于洋葱素的生物测定方法，其包括将足量诱导细胞死亡的量的谷氨酸和用于培养的培养基的Yokukansan 细胞，并从培养细胞在培养基中的活力评价肌营养素的药理活性值。
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公开(公告)号：US08420336B2
公开(公告)日：2013-04-16
申请号：US13000029
申请日：2008-06-27
IPC分类号： G01N33/567
CPC分类号： A61K36/48 , G01N33/566 , G01N2333/70571 , G01N2500/02
摘要： An in-vitro bioassay method for yokukansan, involving competitively binding a labeled ligand and yokukansan with cells or cell membranes expressing glutamate receptors, measuring the binding activity of yokukansan, and determining the pharmacological activity of yokukansan from the measurement.
摘要翻译： 一种体内生物测定方法，涉及用标记的配体和肌苷酸与表达谷氨酸受体的细胞或细胞膜进行竞争性结合的方法，测定肌内兰酸的结合活性，并从测定中测定肌营胺的药理学活性。
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公开(公告)号：US20110027821A1
公开(公告)日：2011-02-03
申请号：US12936282
申请日：2008-04-03
申请人： Yasushi Ikarashi , Zenji Kawakami
发明人： Yasushi Ikarashi , Zenji Kawakami
IPC分类号： C12Q1/02
CPC分类号： A61K36/18 , G01N33/5014 , G01N33/5058
摘要： The invention intends to find out a bioassay system with an in-vitro test capable of ensuring the higher quality of yokukansan, and provides a bioassay method for yokukansan, comprising adding glutamate in an amount sufficient to induce cell death and yokukansan to a medium for culturing cells, and evaluating pharmacological activity value of yokukansan from viability of the cultured cells in the medium.
摘要翻译： 本发明旨在找出具有能够确保更高品质的洋甘康素的体外试验的生物测定系统，并且提供了对于洋葱素的生物测定方法，其包括将足量诱导细胞死亡的量的谷氨酸和用于培养的培养基的Yokukansan 细胞，并从培养细胞在培养基中的活力评价肌营养素的药理活性值。
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7.发明申请METHOD FOR MANUFACTURING ALLOY POWDERS BASED ON TITANIUM, ZIRCONIUM AND HAFNIUM, ALLOYED WITH THE ELEMENTS NI, CU, TA, W, RE, OS AND IR 有权与元素NI，CU，TA，W，RE，OS和IR合金制备基于钛，锆和铪的合金粉末的方法公开(公告)号：US20100313709A1
公开(公告)日：2010-12-16
申请号：US12867501
申请日：2009-02-27
IPC分类号： B22F9/20
CPC分类号： B22F9/20 , B22F2998/00 , B22F2998/10 , B22F2999/00 , C01B6/24 , C22B5/04 , C22B34/1268 , C22B34/1286 , C22B34/14 , C22C1/0458 , C22C9/00 , C22C14/00 , C22C16/00 , C22C19/03 , C22C27/04 , B22F1/0011 , C23F1/00 , B22F2201/11 , B22F2201/013 , B22F2201/12 , B22F2201/02
摘要： A method for manufacturing alloy powders based on titanium, zirconium and hafnium alloyed with the elements Ni, Cu, Ta, W, Re, Os, and Ir is described in which an oxide of Ti and Zr and Hf is mixed with a metal powder of the elements named and with a reducing agent, and wherein this mixture is heated in a furnace, optionally under a argonate atmosphere or, optionally under hydrogen atmosphere until the reducing reaction begins, the reaction product is leached and then washed and dried, wherein the oxide used has an average grain size of 0.5 to 20 μm, a specific surface area according to BET of 0.5 20 m2/g and a minimum content of 94 wet.-%. An easy to produce powder, in particular in relation to the ignition point and burning time, is produced.
摘要翻译： 描述了与元素Ni，Cu，Ta，W，Re，Os和Ir合金化的钛，锆和铪合金粉末的制造方法，其中将Ti和Zr和Hf的氧化物与金属粉末 所述元件命名为还原剂，并且其中将该混合物在炉中任选地在氩气气氛下或任选在氢气氛下加热直到还原反应开始，将反应产物浸出，然后洗涤并干燥，其中氧化物 使用的平均粒径为0.5〜20μm，BET比表面积为0.5±20m2 / g，最小含量为94％。 特别是相对于点火点和燃烧时间产生粉末易于生产。
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公开(公告)号：US08609352B2
公开(公告)日：2013-12-17
申请号：US12867514
申请日：2008-02-15
IPC分类号： G01N33/53
CPC分类号： G01N33/566 , A61K36/076 , A61K36/23 , A61K36/232 , A61K36/233 , A61K36/284 , A61K36/484 , A61K36/74 , G01N2333/70571 , A61K2300/00
摘要： The invention intends to find out an in-vitro bioassay system capable of ensuring qualities of yokukansan to a higher degree, and provides a bioassay method for yokukansan, comprising competitively reacting labeled ligand and a test sample containing yokukansan with cells or cell membranes expressing serotonin 1A receptors, and measuring binding activity of yokukansan from the amount of the labeled ligand bound, and a bioassay method for yokukansan, comprising reacting labeled GTP and a test sample containing yokukansan with cells or cell membranes expressing serotonin 1A receptors, and measuring receptor-agonist activity of yokukansan from the amount of the labeled GTP bound.
摘要翻译： 本发明旨在找出能够更高程度地确保洋葱素质量的体外生物测定系统，并且提供了一个生物测定方法，其中包括对标记的配体进行有竞争力的反应和含有洋甘草素的测试样品与表达5-羟色胺1A的细胞或细胞膜 受体，并测定结合标记的配体的结合活性，以及生物测定方法，包括使标记的GTP与含有亮肌素的测试样品与表达5-羟色胺1A受体的细胞或细胞膜反应并测定受体 - 激动剂活性 的Yokukansan从标记的GTP绑定的量。
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公开(公告)号：US20100317028A1
公开(公告)日：2010-12-16
申请号：US12867514
申请日：2008-02-15
IPC分类号： G01N33/53
CPC分类号： G01N33/566 , A61K36/076 , A61K36/23 , A61K36/232 , A61K36/233 , A61K36/284 , A61K36/484 , A61K36/74 , G01N2333/70571 , A61K2300/00
摘要： The invention intends to find out an in-vitro bioassay system capable of ensuring qualities of yokukansan to a higher degree, and provides a bioassay method for yokukansan, comprising competitively reacting labeled ligand and a test sample containing yokukansan with cells or cell membranes expressing serotonin 1A receptors, and measuring binding activity of yokukansan from the amount of the labeled ligand bound, and a bioassay method for yokukansan, comprising reacting labeled GTP and a test sample containing yokukansan with cells or cell membranes expressing serotonin 1A receptors, and measuring receptor-agonist activity of yokukansan from the amount of the labeled GTP bound.
摘要翻译： 本发明旨在找出能够更高程度地确保洋葱素质量的体外生物测定系统，并且提供了一个生物测定方法，其中包括对标记的配体进行有竞争力的反应和含有洋甘草素的测试样品与表达5-羟色胺1A的细胞或细胞膜 受体，并测定结合标记的配体的结合活性，以及生物测定方法，包括使标记的GTP与含有亮肌素的测试样品与表达5-羟色胺1A受体的细胞或细胞膜反应并测定受体 - 激动剂活性 的Yokukansan从标记的GTP绑定的量。
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