-
公开(公告)号：US11579086B2
公开(公告)日：2023-02-14
申请号：US17496262
申请日：2021-10-07
Applicant: Yan Wang , Cory McCluskey
Inventor： Yan Wang , Cory McCluskey
Abstract: Provided is a fluorescence reader that uses two excitation channels and can read up to seven different fluorescent dyes in a single run. Each excitation channel has one light source and one single excitation filter and one dichroic mirror. One excitation channel is capable of exciting multiple fluorescent dyes and can be used to distinguish multiple dyes in combination with multiple emission filters. The excitation channels are driven by a motor that can automatically switch the two excitation channels for taking images of up to seven different fluorescent dyes. An algorithm to calibrate the crosstalk between different fluorescent dyes is also provided. Also provided is a method for analyzing digital PCR data using a ratio of two fluorescence emission readings.
-
公开(公告)号：US20220026363A1
公开(公告)日：2022-01-27
申请号：US17496285
申请日：2021-10-07
Applicant: Yan Wang , Cory McCluskey
Inventor： Yan Wang , Cory McCluskey
Abstract: Provided is a fluorescence reader that uses two excitation channels and can read up to seven different fluorescent dyes in a single run. Each excitation channel has one light source and one single excitation filter and one dichroic mirror. One excitation channel is capable of exciting multiple fluorescent dyes and can be used to distinguish multiple dyes in combination with multiple emission filters. The excitation channels are driven by a motor that can automatically switch the two excitation channels for taking images of up to seven different fluorescent dyes. An algorithm to calibrate the crosstalk between different fluorescent dyes is also provided. Also provided is a method for analyzing digital PCR data using a ratio of two fluorescence emission readings.
-
公开(公告)号：US20220026362A1
公开(公告)日：2022-01-27
申请号：US17496262
申请日：2021-10-07
Applicant: Yan Wang , Cory McCluskey
Inventor： Yan Wang , Cory McCluskey
Abstract: Provided is a fluorescence reader that uses two excitation channels and can read up to seven different fluorescent dyes in a single run. Each excitation channel has one light source and one single excitation filter and one dichroic mirror. One excitation channel is capable of exciting multiple fluorescent dyes and can be used to distinguish multiple dyes in combination with multiple emission filters. The excitation channels are driven by a motor that can automatically switch the two excitation channels for taking images of up to seven different fluorescent dyes. An algorithm to calibrate the crosstalk between different fluorescent dyes is also provided. Also provided is a method for analyzing digital PCR data using a ratio of two fluorescence emission readings.
-
公开(公告)号：US11549887B2
公开(公告)日：2023-01-10
申请号：US17496285
申请日：2021-10-07
Applicant: Yan Wang , Cory McCluskey
Inventor： Yan Wang , Cory McCluskey
Abstract: Provided is a fluorescence reader that uses two excitation channels and can read up to seven different fluorescent dyes in a single run. Each excitation channel has one light source and one single excitation filter and one dichroic mirror. One excitation channel is capable of exciting multiple fluorescent dyes and can be used to distinguish multiple dyes in combination with multiple emission filters. The excitation channels are driven by a motor that can automatically switch the two excitation channels for taking images of up to seven different fluorescent dyes. An algorithm to calibrate the crosstalk between different fluorescent dyes is also provided. Also provided is a method for analyzing digital PCR data using a ratio of two fluorescence emission readings.
-
公开(公告)号：US11320375B2
公开(公告)日：2022-05-03
申请号：US16894612
申请日：2020-06-05
Applicant: Yan Wang , Cory McCluskey
Inventor： Yan Wang , Cory McCluskey
Abstract: Provided is a fluorescence reader that uses two excitation channels and can read up to seven different fluorescent dyes in a single run. Each excitation channel has one light source and one single excitation filter and one dichroic mirror. One excitation channel is capable of exciting multiple fluorescent dyes and can be used to distinguish multiple dyes in combination with multiple emission filters. The excitation channels are driven by a motor that can automatically switch the two excitation channels for taking images of up to seven different fluorescent dyes. An algorithm to calibrate the crosstalk between different fluorescent dyes is also provided. Also provided is a method for analyzing digital PCR data using a ratio of two fluorescence emission readings.
-
Patent Agency Ranking
公开(公告)号：US20210381980A1
公开(公告)日：2021-12-09
申请号：US16894612
申请日：2020-06-05
Applicant: Yan Wang , Cory McCluskey
Inventor： Yan Wang , Cory McCluskey
Abstract: Provided is a fluorescence reader that uses two excitation channels and can read up to seven different fluorescent dyes in a single run. Each excitation channel has one light source and one single excitation filter and one dichroic mirror. One excitation channel is capable of exciting multiple fluorescent dyes and can be used to distinguish multiple dyes in combination with multiple emission filters. The excitation channels are driven by a motor that can automatically switch the two excitation channels for taking images of up to seven different fluorescent dyes. An algorithm to calibrate the crosstalk between different fluorescent dyes is also provided. Also provided is a method for analyzing digital PCR data using a ratio of two fluorescence emission readings.
-
公开(公告)号：US11761032B2
公开(公告)日：2023-09-19
申请号：US16504065
申请日：2019-07-05
Applicant: Yan Wang , Cory McCluskey
Inventor： Yan Wang , Cory McCluskey
IPC: C12Q1/68 , C12Q1/6851 , C12Q1/6825 , B01L7/00 , B01L3/00
CPC classification number: C12Q1/6851 , B01L3/5027 , B01L7/52 , C12Q1/6825
Abstract: Disclosed are devices that can perform multiple independent digital PCRs with real-time monitoring capability. The device comprises multiple PCR mini-reactors thermally coupled with its own temperature control element, a detection unit, and a motor for moving the PCR mini-reactors or the detection unit. The real-time digital PCR device can simultaneously perform multiple digital PCRs, generate amplification curves of thousands and millions of individual PCR processes, evaluate binary readouts based on the kinetic properties of individual amplification curves, and identify different target sequences based on the amplification curves. Methods of using the real-time digital PCR device to detect target nucleic acids and count circulating tumor cells are also disclosed.
-
公开(公告)号：US20220028490A1
公开(公告)日：2022-01-27
申请号：US17497348
申请日：2021-10-08
Applicant: Yan Wang , Cory McCluskey
Inventor： Yan Wang , Cory McCluskey
IPC: G16B30/00
Abstract: Disclosed are methods for analyzing digital PCR data using real time measurements during the amplification cycles of the dPCR. An endpoint threshold is used to preliminarily separate positive amplifications from negative amplifications for a plurality of microreactions in the dPCR. The preliminary positive amplifications are further evaluated based on properties of the amplification curves of the microreactions so as to remove false positives.
-
公开(公告)号：US20220025450A1
公开(公告)日：2022-01-27
申请号：US17497628
申请日：2021-10-08
Applicant: Yan Wang , Cory McCluskey
Inventor： Yan Wang , Cory McCluskey
IPC: C12Q1/6851 , C12Q1/6825 , B01L7/00 , B01L3/00
Abstract: Disclosed are devices that can perform multiple independent digital PCRs with real-time monitoring capability. The device comprises multiple PCR mini-reactors thermally coupled with its own temperature control element, a detection unit, and a motor for moving the PCR mini-reactors or the detection unit. The real-time digital PCR device can simultaneously perform multiple digital PCRs, generate amplification curves of thousands and millions of individual PCR processes, evaluate binary readouts based on the kinetic properties of individual amplification curves, and identify different target sequences based on the amplification curves. Methods of using the real-time digital PCR device to detect target nucleic acids and count circulating tumor cells are also disclosed.
-
公开(公告)号：US20210350874A1
公开(公告)日：2021-11-11
申请号：US17316666
申请日：2021-05-10
Applicant: Yan Wang , Cory McCluskey
Inventor： Yan Wang , Cory McCluskey
IPC: G16B30/00
Abstract: Disclosed are methods for analyzing digital PCR data using real time measurements during the amplification cycles of the dPCR. An endpoint threshold is used to preliminarily separate positive amplifications from negative amplifications for a plurality of microreactions in the dPCR. The preliminary positive amplifications are further evaluated based on properties of the amplification curves of the microreactions so as to remove false positives.
-
-
-
-
-
-
-
-
-
Search Results
450
records
(took 0.02 seconds)
