公开(公告)号：US09702010B2

公开(公告)日：2017-07-11

申请号：US14175676

申请日：2014-02-07

Applicant: YALE UNIVERSITY

Inventor： Alessandro D. Santin

IPC: A61K38/00 ,  C12Q1/68 ,  A61K38/16 ,  A61K38/48 ,  A61K39/395 ,  A61K45/06 ,  C07K16/30 ,  A61K39/00

CPC classification number: C12Q1/6886 ,  A61K38/164 ,  A61K38/4886 ,  A61K39/395 ,  A61K45/06 ,  A61K2039/505 ,  C07K16/30 ,  C12Q2600/136 ,  C12Q2600/158 ,  A61K2300/00

Abstract: The invention discloses high levels of receptors for Clostridium perfringens enterotoxin (CPE) have been found in ovarian cancer and uterine cancer tissue samples. In addition, successful in vivo treatment of a mouse model of ovarian cancer with intraperitoneal injection of CPE is disclosed. High levels of Ep-CAM protein is also disclosed in ovarian cancer tissue samples. Thus, the invention provides a method of treating ovarian cancer and uterine cancer by administering CPE. The invention also provides a method of treating cancer in a mammal involving intraperitoneal administration of CPE, where at least some cancerous cells are located in or adjacent to the peritoneal cavity of the mammal. The invention also provides a method of treating ovarian cancer involving administering an anti-Ep-CAM antibody. The invention also provides a method of treating cancers expressing claudin-3 or claudin-4 by administering an antibody against claudin-3 and/or an antibody against claudin-4. The invention also provides a method of protecting a mammal from CPE toxicity involving administering a protective agent that binds to claudin-3 and/or claudin-4 and inhibits CPE binding to claudin-3 and/or claudin-4.

公开(公告)号：US20150218644A1

公开(公告)日：2015-08-06

申请号：US14175676

申请日：2014-02-07

Applicant: YALE UNIVERSITY

Inventor： Alessandro D. Santin

IPC: C12Q1/68

CPC classification number: C12Q1/6886 ,  A61K38/164 ,  A61K38/4886 ,  A61K39/395 ,  A61K45/06 ,  A61K2039/505 ,  C07K16/30 ,  C12Q2600/136 ,  C12Q2600/158 ,  A61K2300/00

Abstract: The invention discloses high levels of receptors for Clostridium perfringens enterotoxin (CPE) have been found in ovarian cancer and uterine cancer tissue samples. In addition, successful in vivo treatment of a mouse model of ovarian cancer with intraperitoneal injection of CPE is disclosed. High levels of Ep-CAM protein is also disclosed in ovarian cancer tissue samples. Thus, the invention provides a method of treating ovarian cancer and uterine cancer by administering CPE. The invention also provides a method of treating cancer in a mammal involving intraperitoneal administration of CPE, where at least some cancerous cells are located in or adjacent to the peritoneal cavity of the mammal. The invention also provides a method of treating ovarian cancer involving administering an anti-Ep-CAM antibody. The invention also provides a method of treating cancers expressing claudin-3 or claudin-4 by administering an antibody against claudin-3 and/or an antibody against claudin-4. The invention also provides a method of protecting a mammal from CPE toxicity involving administering a protective agent that binds to claudin-3 and/or claudin-4 and inhibits CPE binding to claudin-3 and/or claudin-4.

Abstract translation: 本发明公开了在卵巢癌和子宫癌组织样品中发现产气荚膜梭菌肠毒素（CPE）的高水平受体。 另外，公开了腹膜内注射CPE对卵巢癌小鼠模型的成功体内治疗。 卵巢癌组织样本中也披露了高水平的Ep-CAM蛋白。 因此，本发明提供了通过施用CPE治疗卵巢癌和子宫癌的方法。 本发明还提供了一种治疗哺乳动物涉及腹膜内给予CPE的癌症的方法，其中至少一些癌细胞位于哺乳动物的腹腔内或邻近腹膜腔。 本发明还提供了治疗卵巢癌的方法，其涉及施用抗Ep-CAM抗体。 本发明还提供了通过施用针对克拉丁蛋白-3的抗体和/或克隆蛋白-4的抗体来治疗表达克拉丁蛋白-3或克拉丁蛋白-4的癌症的方法。 本发明还提供了一种保护哺乳动物免受CPE毒性的方法，包括施用结合克洛丁-3和/或克劳丁-4并抑制CPE与克拉丁蛋白-3和/或克拉丁-4的结合的保护剂。