公开(公告)号：US09868947B2

公开(公告)日：2018-01-16

申请号：US15146621

申请日：2016-05-04

申请人： Washington University

发明人： Christine Gurnett ,  Gabriel Haller ,  Matthew Dobbs ,  David Alvarado

IPC分类号： C12P19/40 ,  C12N15/10

CPC分类号： C12N15/1031 ,  C12N15/102 ,  C12N15/1058 ,  C12N15/1093 ,  C12P19/40 ,  C12Q2525/186 ,  C12Q2521/525 ,  C12Q2521/539 ,  C12Q2525/101 ,  C12Q2525/117 ,  C12Q2531/101 ,  C12Q2531/113

摘要： The present disclosure provides a method of making a systematic single point mutation in a target nucleic acid and a method of generating a mutational library comprising target nucleic acids with single point mutations. The mutational library comprises target nucleic acids with single point mutations distributed evenly throughout the target nucleic acid.

公开(公告)号：US20160326517A1

公开(公告)日：2016-11-10

申请号：US15146621

申请日：2016-05-04

申请人： Washington University

发明人： Christine Gurnett ,  Gabriel Haller ,  Matthew Dobbs ,  David Alvarado

IPC分类号： C12N15/10 ,  C12P19/40

CPC分类号： C12N15/1031 ,  C12N15/102 ,  C12N15/1058 ,  C12N15/1093 ,  C12P19/40 ,  C12Q2525/186 ,  C12Q2521/525 ,  C12Q2521/539 ,  C12Q2525/101 ,  C12Q2525/117 ,  C12Q2531/101 ,  C12Q2531/113

摘要： The present disclosure provides a method of making a systematic single point mutation in a target nucleic acid and a method of generating a mutational library comprising target nucleic acids with single point mutations. The mutational library comprises target nucleic acids with single point mutations distributed evenly throughout the target nucleic acid.

摘要翻译： 本公开内容提供了在靶核酸中进行系统性单点突变的方法和产生包含具有单点突变的靶核酸的突变文库的方法。 突变文库包含具有均匀分布在靶核酸中的单点突变的靶核酸。