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1.发明申请公开(公告)号：US20170183704A1
公开(公告)日：2017-06-29
申请号：US15409137
申请日：2017-01-18
CPC分类号： C12P21/02 , C12N9/14 , C12N15/70 , C12N15/72 , C12Y306/00
摘要： The present invention is concerned with a method of production of authentic human epidermal growth factor (EGF) and hyper-production of authentic basic fibroblast growth factor (bFGF) without any modification at either C- or N-terminal of the bFGF.
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2.发明申请MEANS AND METHOD FOR EXPRESSION OF AUTHENTIC HUMAN EPIDERMAL GROWTH FACTOR AND/OR BASIC FIBROLAST GROWTH FACTOR IN CYTOPLASM AND/OR CULTURE MEDIUM OF ESCHERICHIA COLI 审中-公开用于表达ESCHERICHIA COLI的CYPOPLASM和/或文化培养基中的认可人类生殖因子和/或基本纤维生长因子的方法和方法公开(公告)号：US20160060318A1
公开(公告)日：2016-03-03
申请号：US14781614
申请日：2014-02-25
IPC分类号： C07K14/50 , C07K14/485
CPC分类号： C07K14/503 , C07K14/485 , C12N15/70
摘要： The present invention relates to an engineered biological system, and in particular an Escherichia coli host. The host is incorporated with a DNA construct for production of at least a first polypeptide. The first polypeptide may be an authentic basic fibroblast growth factor (bFGF). The DNA construct has an insert including, for example, in the sequence of, an expression cassette, an intein sequence and DNA coding for the first polypeptide. The DNA construct is configured to effect the host to secrete the basic fibroblast growth factor (bFGF) in the cytoplasm of the host and/or to excrete to cell medium in which the host is cultured.
摘要翻译： 本发明涉及工程生物系统，特别是大肠杆菌宿主。 该宿主与用于产生至少第一多肽的DNA构建体结合。 第一种多肽可能是真正的碱性成纤维细胞生长因子（bFGF）。 DNA构建体具有例如以表达盒，内含肽序列和编码第一多肽的DNA的序列的插入片段。 配置DNA构建物以使宿主分泌宿主细胞质中的碱性成纤维细胞生长因子（bFGF）和/或排泄至其中培养宿主的细胞培养基。
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