公开(公告)号：US09879278B2

公开(公告)日：2018-01-30

申请号：US14382816

申请日：2013-02-27

Applicant: WAKE FOREST UNIVERSITY HEALTH SCIENCES

Inventor： Baisong Lu ,  Qingguo Zhao ,  James Yoo ,  Anthony Atala

IPC: C12N15/85

CPC classification number: C12N15/85 ,  C12N2800/40 ,  C12N2840/007

Abstract: The invention includes compositions and methods for the selective expression of a target gene in a subset of cells. In certain embodiments, the present invention includes a construct comprising a first nucleic acid sequence comprising an episomal maintenance element and a second nucleic acid sequence comprising a target gene wherein the expression of the episomal maintenance element is regulated by a constitutive promoter and the expression of the target gene is regulated by a non-constitutive promoter. The construct is able to maintain episomal state, no matter whether the target gene is expressed in the cell.

公开(公告)号：US20150079682A1

公开(公告)日：2015-03-19

申请号：US14382816

申请日：2013-02-27

Applicant: WAKE FOREST UNIVERSITY HEALTH SCIENCES

Inventor： Baisong Lu ,  Qingguo Zhao ,  James Yoo ,  Anthony Atala

IPC: C12N15/85

CPC classification number: C12N15/85 ,  C12N2800/40 ,  C12N2840/007

Abstract: The invention includes compositions and methods for the selective expression of a target gene in a subset of cells. In certain embodiments, the present invention includes a construct comprising a first nucleic acid sequence comprising an episomal maintenance element and a second nucleic acid sequence comprising a target gene wherein the expression of the episomal maintenance element is regulated by a constitutive promoter and the expression of the target gene is regulated by a non-constitutive promoter. The construct is able to maintain episomal state, no matter whether the target gene is expressed in the cell.

Abstract translation: 本发明包括用于在细胞子集中选择性表达靶基因的组合物和方法。 在某些实施方案中，本发明包括构建体，其包含第一核酸序列，其包含附加型维持元件和包含靶基因的第二核酸序列，其中所述附加型维持元件的表达由组成型启动子调节， 靶基因由非组成型启动子调控。 无论目标基因是否在细胞中表达，该构建体能够保持附加型状态。