公开(公告)号：US5770203A

公开(公告)日：1998-06-23

申请号：US449045

申请日：1995-05-24

申请人： W. Neal Burnette ,  Harvey R. Kaslow

发明人： W. Neal Burnette ,  Harvey R. Kaslow

IPC分类号： A61K39/106 ,  A61K39/00 ,  A61P31/04 ,  C07K14/00 ,  C07K14/195 ,  C07K14/28 ,  C07K14/41 ,  C07K19/00 ,  C12N1/21 ,  C12N15/09 ,  C12N15/31 ,  C12P21/02 ,  C12N9/10

CPC分类号： C07K14/28 ,  A61K39/00 ,  Y10S424/832 ,  Y10S435/909

摘要： The development of subunits and subunit analogs of the cholera exotoxin by recombinant DNA techniques provides vaccine products that can retain their biological activity and immunogenicity, and can confer protection against disease challenge. Genetically-engineered modifications of the subunits result in products that retain immunogenicity, yet are reduced in, or are essentially free of, enzymatic activity associated with toxin reactogenicity.

摘要翻译： 通过重组DNA技术发展霍乱外毒素的亚单位和亚基类似物提供可以保留其生物活性和免疫原性的疫苗产品，并且可以赋予防止疾病挑战的保护。 亚基的遗传工程改造产生保留免疫原性但仍被还原或基本上不含与毒素反应原性相关的酶活性的产物。

公开(公告)号：US5874287A

公开(公告)日：1999-02-23

申请号：US435605

申请日：1995-05-05

申请人： W. Neal Burnette ,  Harvey R. Kaslow

发明人： W. Neal Burnette ,  Harvey R. Kaslow

IPC分类号： A61K39/106 ,  A61K39/00 ,  A61P31/04 ,  C07K14/00 ,  C07K14/195 ,  C07K14/28 ,  C07K14/41 ,  C07K19/00 ,  C12N1/21 ,  C12N15/09 ,  C12N15/31 ,  C12P21/02 ,  C12N1/00 ,  C12N5/10 ,  C12N15/54

CPC分类号： C07K14/28 ,  A61K39/00 ,  Y10S424/832 ,  Y10S435/909

摘要： The development of subunits and subunit analogs of the cholera exotoxin by recombinant DNA techniques provides vaccine products that can retain their biological activity and immunogenicity, and can confer protection against disease challenge. Genetically-engineered modifications of the subunits result in products that retain immunogenicity, yet are reduced in, or are essentially free of, enzymatic activity associated with toxin reactogenicity.

摘要翻译： 通过重组DNA技术发展霍乱外毒素的亚单位和亚基类似物提供可以保留其生物活性和免疫原性的疫苗产品，并且可以赋予防止疾病挑战的保护。 亚基的遗传工程改造产生保留免疫原性但仍被还原或基本上不含与毒素反应原性相关的酶活性的产物。

公开(公告)号：US5165927A

公开(公告)日：1992-11-24

申请号：US682773

申请日：1991-04-09

申请人： Harvey R. Kaslow

发明人： Harvey R. Kaslow

IPC分类号： A61K39/00 ,  C07K14/235

CPC分类号： C07K14/235 ,  A61K39/00

摘要： A modified pertussis toxin suitable as a pertussis vaccine having an essentially unmodified B-oligomer and a catalytic subunit which is inactivated by treatment with polyphosphate compounds, sulfhydryl reductants and mild detergents followed by modification of the activated --SH groups to inhibit ADP-ribosylating activity.

摘要翻译： 适合作为百日咳疫苗的经修饰的百日咳毒素，其具有基本上未修饰的B寡聚体和催化亚单位，其通过用多磷酸盐化合物，巯基还原剂和温和去污剂处理而失活，随后修饰活化的-SH基团以抑制ADP-核糖基化活性。

公开(公告)号：US5032398A

公开(公告)日：1991-07-16

申请号：US893080

申请日：1986-08-01

申请人： Harvey R. Kaslow

发明人： Harvey R. Kaslow

IPC分类号： A61K39/00 ,  C07K14/235

CPC分类号： C07K14/235 ,  A61K39/00 ,  Y10S530/825

摘要： A modified pertussis toxin suitable as a pertussis vaccine having an essentially unmodified B-oligomer and a catalytic subunit which is inactivated by treatment with polyphosphate compounds, sulfhydryl reductants and mild detergents followed by modification of the activated --SH groups to inhibit ADP-ribosylating activity.

摘要翻译： 适合作为百日咳疫苗的修饰的百日咳毒素，其具有基本上未修饰的B寡聚体和催化亚单位，其通过用多磷酸盐化合物，巯基还原剂和温和去污剂处理而失活，随后修饰活化的-SH基团以抑制ADP-核糖基化活性。

公开(公告)号：US07341733B2

公开(公告)日：2008-03-11

申请号：US10314192

申请日：2002-12-06

申请人： Harvey R. Kaslow

发明人： Harvey R. Kaslow

IPC分类号： A61K45/00

CPC分类号： C12N9/1077 ,  A61K38/164 ,  A61K39/39 ,  A61K2039/55544

摘要： Compositions characterized by ADP-ribosyltransferase activity are useful in promoting prophylactic and/or therapeutic responses as are promoted by, e.g., pertussis toxin but directed against another target antigen (e.g., a cancer-related antigen) in a mammalian patient.

摘要翻译： 以ADP-核糖基转移酶活性为特征的组合物可用于促进例如百日咳毒素但针对哺乳动物患者中的另一靶抗原（例如，癌相关抗原）促进的预防和/或治疗应答。

公开(公告)号：US06514499B1

公开(公告)日：2003-02-04

申请号：US09510664

申请日：2000-02-22

申请人： Harvey R. Kaslow

发明人： Harvey R. Kaslow

IPC分类号： A61K3512

CPC分类号： C12N9/1077 ,  A61K38/164 ,  A61K39/39 ,  A61K2039/55544

摘要： Compositions characterized by ADP-ribosyltransferase activity are useful in promoting prophylactic and/or therapeutic responses as are promoted by, e.g., pertussis toxin but directed against another target antigen (e.g., a cancer-related antigen) in a mammalian patient.

公开(公告)号：US6056960A

公开(公告)日：2000-05-02

申请号：US482758

申请日：1995-06-07

申请人： Harvey R. Kaslow

发明人： Harvey R. Kaslow

IPC分类号： A61K38/45 ,  A61K31/00 ,  A61K38/00 ,  A61K38/16 ,  A61K39/00 ,  A61K39/39 ,  A61P35/00 ,  C12N9/10 ,  A61K35/12 ,  A61K35/66 ,  A61K39/10

CPC分类号： C12N9/1077 ,  A61K38/164 ,  A61K39/39 ,  A61K2039/55544

摘要： Compositions characterized by ADP-ribosyltransferase activity are useful in promoting prophylactic and/or therapeutic responses as are promoted by, e.g., pertussis toxin but directed against another target antigen (e.g., a cancer-related antigen) in a mammalian patient.