公开(公告)号：US20150017678A1

公开(公告)日：2015-01-15

申请号：US14254611

申请日：2014-04-16

Applicant: University of Washington Through Its Center for Commercialization

Inventor： Thomas Matula ,  Andrew A. Brayman ,  Oleg A. Sapozhnikov ,  Brian MacConaghy

IPC: G01N1/31 ,  G01N21/53 ,  G01N21/63 ,  G01N21/64

CPC classification number: G01N21/53 ,  B01L3/502723 ,  B01L3/502761 ,  B01L3/502784 ,  B01L2200/0652 ,  B01L2200/0673 ,  B01L2300/0816 ,  B01L2300/0883 ,  B01L2400/0436 ,  G01N1/4077 ,  G01N21/6486 ,  G01N2001/4066 ,  G01N2015/1081 ,  G01N2021/6439

Abstract: Embodiments are generally related to differentiating and/or separating portions of a sample that are of interest from the remainder of the sample. Embodiments may be directed towards separating cells of interest from a cell sample. In some embodiments, acoustic impedances of the cells of interest may be modified. For example, the acoustic properties of the cells of interest may be modified by attaching bubbles to the cells of interest. The cell sample may then be subjected to an acoustic wave. The cells of interest may be differentiated and/or separated from the remainder of the sample based on relative displacements and/or volumetric changes experienced by the cells of interest in response thereto. The cells of interest may be separated using a standing wave and sorted into separate channels of a flow cell. Optionally, the cells may be interrogated by a light source and differentiated by signals generated in response thereto.

Abstract translation: 实施例通常涉及区分和/或分离样品的与样品其余部分有关的部分。 实施方案可以针对将感兴趣的细胞与细胞样品分离。 在一些实施例中，可以修改感兴趣的细胞的声阻抗。 例如，感兴趣的细胞的声学性质可以通过将气泡附着到感兴趣的细胞来修饰。 然后可以对电池样品进行声波。 感兴趣的细胞可以基于相应的位移和/或由感兴趣的细胞响应于其的体积变化而与样品的其余部分分离和/或分离。 感兴趣的细胞可以使用驻波分离并分类成流动池的分开的通道。 可选地，可以通过光源询问单元，并且通过响应于其产生的信号来区分单元。

公开(公告)号：US09645080B2

公开(公告)日：2017-05-09

申请号：US14254611

申请日：2014-04-16

Applicant: University of Washington Through Its Center for Commercialization

Inventor： Thomas Matula ,  Andrew A. Brayman ,  Oleg A. Sapozhnikov ,  Brian MacConaghy ,  Jarred Egan Swalwell ,  Camilo Perez

IPC: G01N33/00 ,  G01N21/53 ,  G01N21/64 ,  G01N1/40 ,  B01L3/00 ,  G01N15/10

CPC classification number: G01N21/53 ,  B01L3/502723 ,  B01L3/502761 ,  B01L3/502784 ,  B01L2200/0652 ,  B01L2200/0673 ,  B01L2300/0816 ,  B01L2300/0883 ,  B01L2400/0436 ,  G01N1/4077 ,  G01N21/6486 ,  G01N2001/4066 ,  G01N2015/1081 ,  G01N2021/6439

Abstract: Embodiments are generally related to differentiating and/or separating portions of a sample that are of interest from the remainder of the sample. Embodiments may be directed towards separating cells of interest from a cell sample. In some embodiments, acoustic impedances of the cells of interest may be modified. For example, the acoustic properties of the cells of interest may be modified by attaching bubbles to the cells of interest. The cell sample may then be subjected to an acoustic wave. The cells of interest may be differentiated and/or separated from the remainder of the sample based on relative displacements and/or volumetric changes experienced by the cells of interest in response thereto. The cells of interest may be separated using a standing wave and sorted into separate channels of a flow cell. Optionally, the cells may be interrogated by a light source and differentiated by signals generated in response thereto.