公开(公告)号：US12085534B2

公开(公告)日：2024-09-10

申请号：US16968535

申请日：2019-02-06

Applicant: University of Houston System

Inventor： Shoujun Xu ,  Yuhong Wang ,  Haina Jia ,  Heng Yin ,  Yujia Mao

IPC: B06B1/02 ,  B06B1/06 ,  G01N27/72 ,  G01N33/15

CPC classification number: G01N27/72 ,  B06B1/0207 ,  B06B1/0622 ,  B06B1/0651 ,  G01N33/15 ,  B06B2201/77

Abstract: A method of measuring dissociation of the biomolecular bonds in one or multiple sample wells using super-resolution force spectroscopy (SURFS). SURFS utilizes precise ultrasound radiation to exert an acoustic radiation force on the biomolecular bonds labeled with magnetic particles. The force-induced dissociation of the protein bonds labeled with magnetic particles may be measured as a reduced magnetic signal by a magnetic sensor. The force resolution allows for differentiating biomolecular bonds with an extremely high level of precision. The biomolecular bonds include protein-protein, protein-nucleic acid, nucleic acid-nucleic acid, small molecule-protein, and small molecule-nucleic acid interactions.

公开(公告)号：US20210041395A1

公开(公告)日：2021-02-11

申请号：US16968535

申请日：2018-02-08

Applicant: University of Houston System

Inventor： Shoujun Xu ,  Yuhong Wang ,  Haina Jia ,  Heng Yin ,  Yujia Mao

IPC: G01N27/72 ,  G01N33/15 ,  B06B1/02 ,  B06B1/06

Abstract: A method of measuring dissociation of the biomolecular bonds in one or multiple sample wells using super-resolution force spectroscopy (SURFS). SURFS utilizes precise ultrasound radiation to exert an acoustic radiation force on the biomolecular bonds labeled with magnetic particles. The force-induced dissociation of the protein bonds labeled with magnetic particles may be measured as a reduced magnetic signal by a magnetic sensor. The force resolution allows for differentiating biomolecular bonds with an extremely high level of precision. The biomolecular bonds include protein-protein, protein-nucleic acid, nucleic acid-nucleic acid, small molecule-protein, and small molecule-nucleic acid interactions.