公开(公告)号：US20230060685A1

公开(公告)日：2023-03-02

申请号：US17822760

申请日：2022-08-26

申请人： Ultima Genomics, Inc.

发明人： Mark Pratt ,  Gilad Almogy ,  Dumitru Brinza ,  Eliane Trepagnier ,  Omer Barad ,  Yoav Etzioni ,  Florian Oberstrass

IPC分类号： C12Q1/6869 ,  G16B30/00 ,  C12Q1/6827

摘要： Described herein are methods of generating a coupled sequencing read pair for a polynucleotide, and methods of analyzing the coupled sequencing read pair. The coupled sequencing read pair can be analyzed to detect polynucleotide variants, including at loci that are not directly sequenced within the coupled sequencing read pair. Other analytical methods can include using coupled sequencing read pairs to construct or validate a consensus sequence. The coupled sequencing read pair may be generated for a polynucleotide by generating sequencing data for a first region by extending a primer using labeled nucleotides; further extending the primer through a second region using nucleotides provided in a second region flow order, wherein primer extension through the second region is faster than primer extension through the first region; and generating sequencing data associated with a sequence of a third region of the polynucleotide by further extending the primer using labeled nucleotides.

公开(公告)号：US11891658B2

公开(公告)日：2024-02-06

申请号：US17394692

申请日：2021-08-05

申请人： Ultima Genomics, Inc.

发明人： Gilad Almogy ,  Florian Oberstrass ,  Omer Barad ,  Chandan Shee

IPC分类号： C12Q1/686

CPC分类号： C12Q1/686 ,  C12Q2563/107 ,  C12Q2563/149 ,  C12Q2563/159

摘要： The present disclosure provides methods and processes for increasing the efficiency and accuracy of nucleic acid sequencing using techniques such as polymerase chain reaction (PCR). The methods described herein can be used to achieve clonal amplification even with a greater than Poisson distribution of beads and/or nucleic acid templates into an emulsion. A PCR method may comprise generating a partition (e.g., a droplet) comprising at least two beads and/or at least two nucleic acid molecules and generating clonal amplification products corresponding to the nucleic acid molecule, at least a subset of which may be attached to a bead.

公开(公告)号：US11459609B2

公开(公告)日：2022-10-04

申请号：US17453481

申请日：2021-11-03

申请人： Ultima Genomics, Inc.

发明人： Mark Pratt ,  Gilad Almogy ,  Dumitru Brinza ,  Eliane Trepagnier ,  Omer Barad ,  Yoav Etzioni ,  Florian Oberstrass

IPC分类号： C12Q1/68 ,  C12Q1/6869 ,  G16B30/00 ,  C12Q1/6827

摘要： Described herein are methods of generating a coupled sequencing read pair for a polynucleotide, and methods of analyzing the coupled sequencing read pair. The coupled sequencing read pair can be analyzed to detect polynucleotide variants, including at loci that are not directly sequenced within the coupled sequencing read pair. Other analytical methods can include using coupled sequencing read pairs to construct or validate a consensus sequence. The coupled sequencing read pair may be generated for a polynucleotide by generating sequencing data for a first region by extending a primer using labeled nucleotides; further extending the primer through a second region using nucleotides provided in a second region flow order, wherein primer extension through the second region is faster than primer extension through the first region; and generating sequencing data associated with a sequence of a third region of the polynucleotide by further extending the primer using labeled nucleotides.