公开(公告)号：US20240318243A1

公开(公告)日：2024-09-26

申请号：US18435829

申请日：2024-02-07

Applicant: Ultima Genomics, Inc.

Inventor： Florian OBERSTRASS ,  Tyson CLARK ,  Caterina SCHWEIDENBACK ,  Daniel MAZUR

IPC: C12Q1/6869 ,  C12Q1/6844

CPC classification number: C12Q1/6869 ,  C12Q1/6844

Abstract: The present disclosure provides compositions, methods, and systems for preparing nucleic acid samples for sequencing, including attaching adapters to template inserts, attaching template nucleic acid molecules to a support, enrichment. Further provided herein are methods of error correction by preserving both strands of a template nucleic acid molecule during amplification.

公开(公告)号：US20230340570A1

公开(公告)日：2023-10-26

申请号：US18114091

申请日：2023-02-24

Applicant: Ultima Genomics, Inc.

Inventor： Daniel MAZUR ,  Kevin HEINEMANN ,  Theo NIKIFOROV ,  Robert ONO ,  Aklilu WORKU ,  Florian OBERSTRASS

IPC: C12Q1/6811

CPC classification number: C12Q1/6811

Abstract: The present disclosure provides methods, systems, compositions, and kits for reducing particle aggregation and improving sequencing quality. A method for processing particles may comprise the use of a first buffer solution or a second buffer solution. A method for processing particles may comprise the use of a single-strand binding moiety such as a single-strand binding protein.

公开(公告)号：US20250027154A1

公开(公告)日：2025-01-23

申请号：US18803028

申请日：2024-08-13

Applicant: ULTIMA GENOMICS, INC.

Inventor： Zohar SHIPONY ,  Florian OBERSTRASS ,  Doron LIPSON ,  Eti MEIRI ,  Tommie Lloyd LINCECUM, JR. ,  Daniel MAZUR ,  Omer BARAD

IPC: C12Q1/6876 ,  C12Q1/6869

Abstract: Nucleic acid molecule constructs, methods of making such construct, and methods of sequencing using such constructs are described herein. The nucleic acid molecule constructs can include a template sequence and a copy of the template sequence, along with a sequencing primer region associated with the template sequence and a copy of the sequencing primer region associated with the copy of the template sequence, which allows the template sequence and the copy of the template sequence to be simultaneously sequenced. Also described are methods of using the nucleic acid molecule constructs for methylation sequencing.

公开(公告)号：US20240417718A1

公开(公告)日：2024-12-19

申请号：US18745437

申请日：2024-06-17

Applicant: Ultima Genomics, Inc.

Inventor： Tyson CLARK ,  Tommie Lloyd LINCECUM, JR. ,  Daniel MAZUR

IPC: C12N15/10

Abstract: Provided herein are systems, methods, compositions, and kits for library preparation. In some cases, multiple distinct types of adapter molecules may be provided to a template nucleic acid molecule. In some cases, a single type of adapter molecule may be provided to a template molecule. In some cases, multiple distinct types of adapter molecules may be sequentially provided to a template molecule to form multi-adapter template complexes.

公开(公告)号：US20240401130A1

公开(公告)日：2024-12-05

申请号：US18738596

申请日：2024-06-10

Applicant: Ultima Genomics, Inc.

Inventor： Florian OBERSTRASS ,  Daniel MAZUR

IPC: C12Q1/6874 ,  C12Q1/44 ,  C12Q1/6876

Abstract: Provided herein are systems, methods, compositions, and kits for sequencing with multi-priming. In some cases, multiple distinct primer molecules may be provided to a template nucleic acid to hybridize to distinct regions of the template nucleic acid. In some cases, a connected primer molecule may be provided to a template nucleic acid to have multiple distinct primer regions hybridize to distinct regions of the template nucleic acid. Non-adjacent regions of the template nucleic acid may be sequenced in distinct sequencing operations.

公开(公告)号：US20230332226A1

公开(公告)日：2023-10-19

申请号：US18176418

申请日：2023-02-28

Applicant: Ultima Genomics, Inc.

Inventor： Florian OBERSTRASS ,  Theo NIKIFOROV ,  Mark STAPLETON ,  Daniel MAZUR ,  Gilad ALMOGY ,  Ishminder MANN ,  Chandan SHEE ,  Benjamin SCHROEDER

IPC: C12Q1/6874 ,  C12Q1/6855

CPC classification number: C12Q1/6874 ,  C12Q1/6855

Abstract: The present disclosure provides compositions, methods, and systems for enrichment and analysis of template nucleic acid molecules, e.g., of a biological sample. Further provided herein are methods of using a plurality of unique bead species each comprising a unique set of identical primer molecules.

公开(公告)号：US20240287483A1

公开(公告)日：2024-08-29

申请号：US18651565

申请日：2024-04-30

Applicant: Ultima Genomics, Inc.

Inventor： Daniel MAZUR ,  Eileen Collins TOZER ,  Theo NIKIFOROV ,  Denise Sanchez TOPACIO-HALL

IPC: C12N9/12 ,  C12Q1/6825

CPC classification number: C12N9/1252 ,  C12Q1/6825 ,  C12Y207/07007

Abstract: Mutant nucleic acid polymerases are described herein. Also described are methods for screening or assaying the mutant nucleic acid polymerases.

公开(公告)号：US20240117411A1

公开(公告)日：2024-04-11

申请号：US18540604

申请日：2023-12-14

Applicant: Ultima Genomics, Inc.

Inventor： Robert ONO ,  Daniel MAZUR ,  Florian OBERSTRASS

IPC: C12Q1/6806

CPC classification number: C12Q1/6806

Abstract: Methods of controlling the density or number of nucleic acid molecules coupled to solid supports are provided. Capture substrates produced thereby or comprising a predetermined density or number of nucleic acids coupled thereto are also provided.

公开(公告)号：US20230062391A1

公开(公告)日：2023-03-02

申请号：US17814780

申请日：2022-07-25

Applicant: ULTIMA GENOMICS, INC.

Inventor： Daniel MAZUR ,  Florian OBERSTRASS

IPC: C12Q1/6806 ,  C12Q1/6853 ,  C12Q1/6874

Abstract: The present disclosure provides compositions comprising nucleic acid molecules coupled to supports and comprising one or more cleavable or excisable moieties. Methods of enriching and sequencing nucleic acid molecules are also provided.

公开(公告)号：US20240360501A1

公开(公告)日：2024-10-31

申请号：US18737230

申请日：2024-06-07

Applicant: Ultima Genomics, Inc.

Inventor： Florian OBERSTRASS ,  Tyson CLARK ,  Caterina SCHWEIDENBACK ,  Daniel MAZUR

IPC: C12Q1/6869 ,  C12Q1/6844

CPC classification number: C12Q1/6869 ,  C12Q1/6844

Abstract: The present disclosure provides compositions, methods, and systems for preparing nucleic acid samples for sequencing, including attaching adapters to template inserts, attaching template nucleic acid molecules to a support, enrichment. Further provided herein are methods of error correction by preserving both strands of a template nucleic acid molecule during amplification.