公开(公告)号：US20240218416A1

公开(公告)日：2024-07-04

申请号：US18562972

申请日：2022-05-20

申请人： UNIVERSITY OF LIMERICK

发明人： Maurice COLLINS ,  Mario Culebras RUBIO ,  Anne Beaucamp MCLOUGHLIN

IPC分类号： C12Q1/00 ,  C12N11/02 ,  D01F9/17 ,  D06M16/00 ,  D06M101/40 ,  G01N27/327

CPC分类号： C12Q1/006 ,  C12N11/02 ,  D01F9/17 ,  D06M16/003 ,  G01N27/3271 ,  G01N27/3276 ,  D06M2101/40 ,  D10B2509/00

摘要： An electrode for an electrochemical sensor device. The electrode comprises a substrate, a carbon nanofibre layer and an enzyme immobilised on the carbon nanofibre layer. The carbon nanofibre layer comprises mesoporous carbon nanofibers and the enzyme is immobilised in the pores of the mesoporous carbon nanofibers. The carbon nanofibre layer is formed from lignin and a second polymeric material, wherein the second polymeric material is immiscible with lignin, through a process of stabilisation and carbonisation which provides a conductive carbon nanofibre framework comprising mesopores suitable for immobilisation of the enzyme. The enzyme immobilised in the carbon nanofibre layer can function by interacting with a target compound or biomarker in a sample solution applied to the electrode which produces a measurable electrochemical change in the electrode. A method of forming the electrode, a sensor device comprising the electrode, a use of a mesoporous carbon nanofibre material for immobilising an enzyme in a sensor device and a method of detecting a target compound or biomarker using the electrode are also disclosed. An electrode for an electrochemical sensor device. The electrode comprises a substrate, a carbon nanofibre layer and an enzyme immobilised on the carbon nanofibre layer. The carbon nanofibre layer comprises mesoporous carbon nanofibers and the enzyme is immobilised in the pores of the mesoporous carbon nanofibers. The carbon nanofibre layer is formed from lignin and a second polymeric material, wherein the second polymeric material is immiscible with lignin, through a process of stabilisation and carbonisation which provides a conductive carbon nanofibre framework comprising mesopores suitable for immobilisation of the enzyme. The enzyme immobilised in the carbon nanofibre layer can function by interacting with a target compound or biomarker in a sample solution applied to the electrode which produces a measurable electrochemical change in the electrode. A method of forming the electrode, a sensor device comprising the electrode, a use of a mesoporous carbon nanofibre material for immobilising an enzyme in a sensor device and a method of detecting a target compound or biomarker using the electrode are also disclosed.