公开(公告)号：US11976092B2

公开(公告)日：2024-05-07

申请号：US16635312

申请日：2018-11-12

Applicant: Ohio State Innovation Foundation ,  UNIVERSITY OF KENTUCKY RESEARCH FOUNDATION

Inventor： Peixuan Guo ,  Mario Vieweger ,  Xin Li ,  Sijin Guo ,  Hongran Yin ,  Xijun Piao ,  Yi Shu ,  Dan Shu ,  Mehdi Rajabi

IPC: C07H21/02 ,  A61K45/06 ,  A61K47/54 ,  A61K47/55 ,  A61K51/06 ,  C12N15/115

CPC classification number: C07H21/02 ,  A61K47/549 ,  A61K47/55 ,  C12N15/115 ,  A61K45/06 ,  A61K51/06 ,  C12N2310/35 ,  C12N2310/351 ,  C12N2310/3519 ,  C12N2330/30

Abstract: Disclosed herein are high Tm RNA nanostructures that can be composed of one or more modules or motifs to build RNA nanostructures with or without layers. The RNA nanostructures can have a core domain and three or more double-stranded arms and formulations thereof to conjugate high copy numbers of therapeutics, pH responsive or enzyme cleavable drug cargo. Also described herein is a design strategy for generation of synthetic RNA oligonucleotides that can self assemble into highly thermostable RNA structures. Also described herein are uses of the RNA nanostructures described herein.

公开(公告)号：US20210115083A1

公开(公告)日：2021-04-22

申请号：US16813087

申请日：2020-03-09

Applicant: University of Kentucky Research Foundation

Inventor： Peixuan Guo ,  Carlo M. Croce ,  Tae Jin Lee ,  Farzin Haque ,  Hui Li

IPC: C07H21/02 ,  C12N15/113 ,  A61K47/55 ,  C12N15/11 ,  A61K47/69 ,  A61P35/00 ,  C12N15/115

Abstract: The presently-disclosed subject matter relates to an artificial RNA nanostructure molecule and method to treat brain tumor in a subject. More particularly, the presently disclosed subject matter relates to a RNA nanostructure containing a multiple branched RNA nanoparticle, a brain tumor targeting module, and an effective amount of a therapeutic agent. Further, the presently disclosed subject matter relates to a method of using the RNA nanostructure composition to treat brain tumor in a subject having or at risk of having brain tumor.

公开(公告)号：US20190390200A1

公开(公告)日：2019-12-26

申请号：US16265154

申请日：2019-02-01

Applicant: UNIVERSITY OF KENTUCKY RESEARCH FOUNDATION

Inventor： Peixuan Guo ,  Hui Li ,  Emil Khisamutdinov ,  Daniel Jasinski

IPC: C12N15/117 ,  A61K39/39

Abstract: The present invention is directed towards an artificial RNA nanostructure comprising multiple external strands of RNA, each external strand comprising about 40-50 nucleotides; one internal strand of RNA comprising more than about 50 nucleotides; the internal strands and external strands assembled to form a triangle nanostructure, a square nanostructure, or a polygon nanostructure and a pRNA three-way junction (3WJ) motif at each vertex of the nanostructure. Such nanostructure can be provided in a composition together with an adjuvant for use in inducing the production of high affinity neutralizing antibodies or inhibitory antibodies, inducing the production of cytokines, inducing an immune response in a subject, or a combination thereof.

公开(公告)号：US20170175122A1

公开(公告)日：2017-06-22

申请号：US15383575

申请日：2016-12-19

Applicant: UNIVERSITY OF KENTUCKY RESEARCH FOUNDATION

Inventor： Peixuan Guo ,  Hui Li ,  Emil Khisamutdinov ,  Daniel Jasinski

IPC: C12N15/117 ,  A61K39/39

CPC classification number: C12N15/117 ,  A61K39/39 ,  A61K2039/55561 ,  A61K2039/6025 ,  A61K2039/64 ,  C12N2310/17 ,  C12N2310/3519 ,  C12N2310/52

Abstract: The present invention is directed towards an artificial RNA nanostructure comprising multiple external strands of RNA, each external strand comprising about 40-50 nucleotides; one internal strand of RNA comprising more than about 50 nucleotides; the internal strands and external strands assembled to form a triangle nanostructure, a square nanostructure, or a polygon nanostructure and a pRNA three-way junction (3WJ) motif at each vertex of the nanostructure. Such nanostructure can be provided in a composition together with an adjuvant for use in inducing the production of high affinity neutralizing antibodies or inhibitory antibodies, inducing the production of cytokines, inducing an immune response in a subject, or a combination thereof.

公开(公告)号：US10648966B2

公开(公告)日：2020-05-12

申请号：US15543821

申请日：2016-01-15

Applicant: UNIVERSITY OF KENTUCKY RESEARCH FOUNDATION

Inventor： Peixuan Guo ,  Shaoying Wang

IPC: C07K14/005 ,  G01N33/487 ,  C12Q1/6869 ,  G01N27/447

Abstract: A conductive channel-containing membrane includes a membrane layer, and a SPP1 connector polypeptide variant that is incorporated into the membrane layer to form an aperture through which conductance can occur when an electrical potential is applied across the membrane. A method of sensing a molecule, such as a polypeptide or nucleic acid molecule, makes use of the conductive channel-containing membrane. A method of DNA sequence makes use of the conductive channel-containing membrane.

公开(公告)号：US10378018B2

公开(公告)日：2019-08-13

申请号：US15383575

申请日：2016-12-19

Applicant: UNIVERSITY OF KENTUCKY RESEARCH FOUNDATION

Inventor： Peixuan Guo ,  Hui Li ,  Emil Khisamutdinov ,  Daniel Jasinski

IPC: C07H21/04 ,  C12N15/117 ,  A61K39/39 ,  A61K39/00

Abstract: The present invention is directed towards an artificial RNA nanostructure comprising multiple external strands of RNA, each external strand comprising about 40-50 nucleotides; one internal strand of RNA comprising more than about 50 nucleotides; the internal strands and external strands assembled to form a triangle nanostructure, a square nanostructure, or a polygon nanostructure and a pRNA three-way junction (3WJ) motif at each vertex of the nanostructure. Such nanostructure can be provided in a composition together with an adjuvant for use in inducing the production of high affinity neutralizing antibodies or inhibitory antibodies, inducing the production of cytokines, inducing an immune response in a subject, or a combination thereof.

公开(公告)号：US20190032050A1

公开(公告)日：2019-01-31

申请号：US15556946

申请日：2016-03-09

Applicant: Daxiang Cui ,  Chunlei Zhang ,  UNIVERSITY OF KENTUCKY RESEARCH FOUNDATION

Inventor： Peixuan Guo ,  Daxiang Cui ,  Dan Shu ,  Yi Shu ,  Chunlei Zhang

IPC: C12N15/113 ,  C12N15/115 ,  A61P35/00

CPC classification number: C12N15/113 ,  A61P35/00 ,  C07H21/04 ,  C12N15/111 ,  C12N15/115 ,  C12N2310/14 ,  C12N2310/16 ,  C12N2310/3519 ,  C12N2310/52 ,  C12N2320/32

Abstract: The presently-disclosed subject matter relates to RNA-based composition and method to treat gastric cancer in a subject. More particularly, the presently disclosed subject matter relates to a RNA nanostructure and composition containing a multiple branched RNA nanoparticle, a gastric cancer targeting module, and an effective amount of a therapeutic agent. Further, the presently disclosed subject matter relates to a method of using the RNA nanoparticle composition to treat gastric cancer in a subject having or at risk of having gastric cancer.

公开(公告)号：US11964028B2

公开(公告)日：2024-04-23

申请号：US17093038

申请日：2020-11-09

Applicant: UNIVERSITY OF KENTUCKY RESEARCH FOUNDATION

Inventor： Peixuan Guo ,  Hui Li ,  Wei Luo

IPC: C07H21/02 ,  A61K51/04 ,  A61K51/12 ,  A61P35/00 ,  C12N15/113 ,  C12N15/115 ,  B82Y5/00

CPC classification number: A61K51/0491 ,  A61K51/12 ,  A61P35/00 ,  C12N15/113 ,  C12N15/115 ,  B82Y5/00 ,  C12N2310/11 ,  C12N2310/113 ,  C12N2310/122 ,  C12N2310/14 ,  C12N2310/141 ,  C12N2310/16 ,  C12N2310/3517 ,  C12N2310/3519 ,  C12N2310/52 ,  C12N2310/322 ,  C12N2310/3533

Abstract: The presently-disclosed subject matter relates to an artificial RNA nanostructure and method of use thereof. In particular, the presently-disclosed subject matter relates to RNA nanoparticles and RNA dendrimers, and methods of disease diagnosis and treatments using the RNA nanostructure and RNA dendrimers.

公开(公告)号：US11325939B2

公开(公告)日：2022-05-10

申请号：US16813087

申请日：2020-03-09

Applicant: University of Kentucky Research Foundation

Inventor： Peixuan Guo ,  Carlo M. Croce ,  Tae Jin Lee ,  Farzin Haque ,  Hui Li

IPC: C07H21/04 ,  C07H21/02 ,  C12N15/113 ,  A61K47/55 ,  C12N15/11 ,  A61K47/69 ,  A61P35/00 ,  C12N15/115 ,  B82Y5/00

Abstract: The presently-disclosed subject matter relates to an artificial RNA nanostructure molecule and method to treat brain tumor in a subject. More particularly, the presently disclosed subject matter relates to a RNA nanostructure containing a multiple branched RNA nanoparticle, a brain tumor targeting module, and an effective amount of a therapeutic agent. Further, the presently disclosed subject matter relates to a method of using the RNA nanostructure composition to treat brain tumor in a subject having or at risk of having brain tumor.

公开(公告)号：US10584144B2

公开(公告)日：2020-03-10

申请号：US15554360

申请日：2016-03-09

Applicant: UNIVERSITY OF KENTUCKY RESEARCH FOUNDATION

Inventor： Peixuan Guo ,  Carlo M. Croce ,  Tae Jin Lee ,  Farzin Haque ,  Hui Li

IPC: C07H21/04 ,  C07H21/02 ,  C12N15/113 ,  A61K47/55 ,  C12N15/11 ,  A61K47/69 ,  A61P35/00 ,  C12N15/115 ,  B82Y5/00

Abstract: The presently-disclosed subject matter relates to an artificial RNA nanostructure molecule and method to treat brain tumor in a subject. More particularly, the presently disclosed subject matter relates to a RNA nanostructure containing a multiple branched RNA nanoparticle, a brain tumor targeting module, and an effective amount of a therapeutic agent. Further, the presently disclosed subject matter relates to a method of using the RNA nanostructure composition to treat brain tumor in a subject having or at risk of having brain tumor.