公开(公告)号：US11261470B2

公开(公告)日：2022-03-01

申请号：US15959498

申请日：2018-04-23

Applicant: UCB BIOPHARMA SRL

Inventor： Philip Jonathan Bassett ,  David Paul Humphreys ,  Pareshkumar Manjibhai Patel

IPC: C12P21/00 ,  C12P21/02 ,  C12N9/52 ,  C07K16/28 ,  C12N15/70

Abstract: The present disclosure relates to a recombinant gram-negative bacterial cell comprising: a) a mutant spr gene encoding a spr protein having a mutation at one or more amino acids selected from D133, H145, H157, N31, R62, I70, Q73, C94, S95, V98, Q99, R100, L108, Y115, V135, L136, G140, R144 and G147 and b) a gene capable of expressing or overexpressing one or more proteins capable of facilitating protein folding, such as FkpA, Skp, SurA, PPiA and PPiD, wherein the cell has reduced Tsp protein activity compared to a wild-type cell, methods employing the cells, use of the cells in the expression of proteins in particular antibodies, such as anti FcRn antibodies and proteins made by the methods described herein.