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公开(公告)号：US11786593B2
公开(公告)日：2023-10-17
申请号：US17078127
申请日：2020-10-23
Applicant: UCB BIOPHARMA SRL
Inventor： Sam Philip Heywood , Gavin Barry Wild , Razwan Hanif , Christopher John Le Page
IPC: A61K39/395 , C07K1/113 , C07K16/28 , C07K16/18 , C07K5/12 , C07C323/12 , C07C323/25
CPC classification number: A61K39/39591 , C07K1/1133 , C07K1/1136 , C07K16/18 , C07K16/2878 , C07C323/12 , C07C323/25 , C07K5/123 , C07K2317/31
Abstract: The present invention provides method of increasing the percentage of monomer in a composition of recombinantly expressed antibody molecules characterised in that the antibody molecule comprises at least one Fv with specificity for an antigen of interest comprising one VH and one VL wherein said VH and VL are connected directly or indirectly via one or more linkers and are stabilised by a disulfide bond therebetween, said method comprises: a) a conversion step of treating the composition with a denaturant selected from urea and/or Guanidine hydrochloride; b) wherein step a) is performed in the presence of a reducing agent or after treatment with a reducing agent.
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公开(公告)号：US11834514B2
公开(公告)日：2023-12-05
申请号：US17078130
申请日：2020-10-23
Applicant: UCB BIOPHARMA SRL
Inventor： Sam Philip Heywood , Gavin Barry Wild , Christopher John Le Page , Razwan Hanif
CPC classification number: C07K16/468 , C07K16/18 , C07K16/2878 , C07K2317/14 , C07K2317/31 , C07K2317/52 , C07K2317/55 , C07K2317/565 , C07K2317/624 , C07K2317/66
Abstract: The present invention provides method for increasing the percentage of monomer in a composition of recombinantly expressed antibody molecules characterised in that the antibody molecule comprises at least one Fv with specificity for an antigen of interest comprising one VH and one VL wherein said VH and VL are connected directly or indirectly via one or more linkers and are stabilised by a disulfide bond therebetween, said method comprises a) a thermal conversion step of holding the composition comprising the antibody molecule at a temperature in the range 30 to 60° C. for a period of at least 1 hour, wherein step a) is performed in the presence of a reducing agent or after treatment with a reducing agent.
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公开(公告)号：US10927164B2
公开(公告)日：2021-02-23
申请号：US15566231
申请日：2016-04-20
Applicant: UCB BIOPHARMA SRL
Inventor： Sam Philip Heywood , Gavin Barry Wild
Abstract: The present invention provides a method for recovering a human VH3 domain-containing antibody in monomeric form. In particular the present invention provides a new method that allows recovery of monomeric human VH3 domain-containing antibodies from a mixture containing monomeric and multimeric forms of the antibody.
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