公开(公告)号：US12077796B2

公开(公告)日：2024-09-03

申请号：US16615419

申请日：2018-05-29

Applicant: UCB BIOPHARMA SRL

Inventor： Bassem Ben Yahia ,  Laetitia Malphettes ,  Nadine Kochanowski ,  Gill Renner ,  Sandrine Durran ,  Andrew Jeffrey Yates

IPC: C12P21/02 ,  C07K1/16 ,  C07K16/00 ,  C12N5/00

CPC classification number: C12P21/02 ,  C07K1/16 ,  C07K16/00 ,  C12N5/0018 ,  C07K2317/14 ,  C07K2317/565 ,  C12N2500/32 ,  C12N2510/02 ,  C12N2511/00 ,  C12N2523/00

Abstract: The present invention relates to the use of limited amounts of cysteine and tryptophan in the cell culture medium during production of recombinant proteins, and in particular antibodies. Proteins and antibodies produced under such controlled conditions exhibit reduced heterogeneity, in particular reduced charge variants heterogeneity.