公开(公告)号：US12203143B2

公开(公告)日：2025-01-21

申请号：US17588196

申请日：2022-01-28

Applicant: THE TRANSLATIONAL GENOMICS RESEARCH INSTITUTE ,  ARIZONA BOARD OF REGENTS ON BEHALF OF NORTHERN ARIZONA UNIVERSITY

Inventor： Jolene Bowers ,  Elizabeth Driebe ,  David Engelthaler ,  Paul Keim ,  Darrin Lemmer

IPC: C12Q1/689 ,  C12Q1/6827 ,  C12Q1/6853 ,  C12Q1/686

Abstract: The present invention provides a method of detecting one or more Klebsiella species within a sample from a subject, the method comprising: subjecting DNA and/or RNA from the sample to a PCR amplification reaction using primer pairs targeting species-specific canonical single nucleotide polymorphisms (canSNPs); and analyzing amplification products resulting from the PCR amplification reaction to detect the one or more Klebsiella species. The present invention also provides a kit for detection of one or more Klebsiella species, Klebsiella clonal groups, AMR genes, and/or virulence genes, the kit comprising primer pairs targeting species-specific canSNPs, K. pneumoniae genes M1 and M2, clonal group-specific canSNPs, AMR genes, and/or virulence genes.

公开(公告)号：US20230094433A1

公开(公告)日：2023-03-30

申请号：US17937117

申请日：2022-09-30

Applicant: THE TRANSLATIONAL GENOMICS RESEARCH INSTITUTE

Inventor： William Tanner Porter ,  Erin Kelley ,  Jolene Bowers ,  David Engelthaler

IPC: C12Q1/6853 ,  C12Q1/686 ,  C12Q1/6851

Abstract: Methods, kits, and oligonucleotides used in the detection of the coronavirus strain, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), are disclosed. In some aspects, the oligonucleotides are primers or probes used in the described methods or kits. The oligonucleotide consists of 40 or less nucleotides and has a nucleotide sequence that consists essentially of, or is a variant of, the nucleotide sequence of: SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9, or SEQ ID NO:10.

公开(公告)号：US20230043710A1

公开(公告)日：2023-02-09

申请号：US17931893

申请日：2022-09-13

Applicant: THE TRANSLATIONAL GENOMICS RESEARCH INSTITUTE

Inventor： David Engelthaler ,  Jolene Bowers ,  James Schupp

IPC: C12Q1/6874 ,  C12Q1/686 ,  C12Q1/682

Abstract: Methods, kits, and oligonucleotides used in the detection of the coronavirus strain, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), are disclosed. In some aspects, the oligonucleotides are primers or probes used in the described methods or kits. The oligonucleotide consists of 40 or less nucleotides and has a nucleotide sequence that consists essentially of, or is a variant of, the nucleotide sequence of: SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:5, SEQ ID NO:6, or SEQ ID NO:7. In some embodiments, the oligonucleotide is modified with an internal spacer or a detectable label. For example, the 5′ terminus is labeled with a fluorophore and the 3′ terminus is complexed to a quencher of fluorescence of said fluorophore. In some embodiments, the nucleotide sequence of the oligonucleotide further comprises a universal tail sequence.

公开(公告)号：US20220325324A1

公开(公告)日：2022-10-13

申请号：US17745832

申请日：2022-05-16

Applicant: THE TRANSLATIONAL GENOMICS RESEARCH INSTITUTE ,  ARIZONA BOARD OF REGENTS ON BEHALF OF NORTHERN ARIZONA UNIVERSITY

Inventor： Elizabeth Driebe ,  Paul Keim ,  David Engelthaler ,  Jolene Bowers ,  Nathan C. Nieto

IPC: C12Q1/689 ,  C12Q1/6883

Abstract: The present invention relates to method of detecting and characterizing one or more Borrelia species causing Lyme Disease or tick-borne relapsing fever within a sample from a subject, the method comprising: a) subjecting DNA and/or RNA from the sample to a PCR amplification reaction using primer pairs targeting at least one region of Borrelia 16S rRNA and at least one region of flaB, ospA, ospB, ospC, glpQ, 16S-23S intergenic spacer (IGS1), 5S-23S intergenic spacer (IGS2), bbk32, dbpA, dbpB, and/or p66; and b) analyzing amplification products resulting from the PCR amplification reaction to detect the one or more Borrelia species.

公开(公告)号：US10745764B2

公开(公告)日：2020-08-18

申请号：US16005012

申请日：2018-06-11

Applicant: THE TRANSLATIONAL GENOMICS RESEARCH INSTITUTE ,  ARIZONA BOARD OF REGENTS ON BEHALF OF NORTHERN ARIZONA UNIVERSITY

Inventor： David Engelthaler ,  Elizabeth Driebe ,  Erin Kelley ,  Paul Keim

IPC: C12Q1/689

Abstract: Embodiments of the invention provide a method of genotyping a C. gattii sample, which can include forming a plurality of mixtures for nucleic amplification. The method can include amplification of specific sequences within the C. gattii genome that can provide definitive genotype information to distinguish between one or more types or subtypes of C. gattii.

公开(公告)号：US20200165663A1

公开(公告)日：2020-05-28

申请号：US16607716

申请日：2018-04-26

Applicant: THE TRANSLATIONAL GENOMICS RESEARCH INSTITUTE ,  ARIZONA BOARD OF REGENTS ON BEHALF OF NORTHERN ARIZONA UNIVERSITY ,  CENTERS FOR DISEASE CONTROL AND PREVENTION (CDC)

Inventor： Elizabeth Driebe ,  Jolene Bowers ,  David Engelthaler ,  Paul Keim ,  Brandi Limbago ,  James K. Rasheed ,  Linda McDougal ,  Valerie S. Albrecht

IPC: C12Q1/689 ,  C12Q1/686

Abstract: The present invention provides a method of detecting Staphylococcus aureus in a subject, by contacting a sample obtained from the subject with at least one detectably labeled probe of the invention or detecting in the sample identity to a sequence of the invention. The invention is also directed to kits, microarrays and detectable Staphylococcus aureus polynucleotide probes useful in detecting the presence of Staphylococcus aureus.

公开(公告)号：US10550427B2

公开(公告)日：2020-02-04

申请号：US15034544

申请日：2014-11-10

Applicant: THE TRANSLATIONAL GENOMICS RESEARCH INSTITUTE ,  ARIZONA BOARD OF REGENTS ON BEHALF OF NORTHERN ARIZONA UNIVERSITY

Inventor： James M. Schupp ,  Rebecca E. Colman ,  David Engelthaler ,  John Gillece ,  Nathan Hicks ,  Paul S. Keim

IPC: C12P19/34 ,  C12Q1/686 ,  C12Q1/6874

Abstract: Some embodiments of the invention include a method of preparing a sample for sequencing that includes receiving a sample and amplifying at least one marker within the sample. In some embodiments, amplification of the first marker may include mixing the sample with a first oligonucleotide that comprises a first universal tail sequence and a second oligonucleotide that comprises a second universal tail sequence. In some aspects of the invention, the first universal tail sequence and the second universal tail sequence are different sequences.

公开(公告)号：US20160326603A1

公开(公告)日：2016-11-10

申请号：US15224044

申请日：2016-07-29

Applicant: THE TRANSLATIONAL GENOMICS RESEARCH INSTITUTE ,  ARIZONA BOARD OF REGENTS ON BEHALF OF NORTHERN ARIZONA UNIVERSITY

Inventor： Bridget Barker ,  Elizabeth Driebe ,  David Engelthaler ,  Paul Keim ,  Jolene Bowers

IPC: C12Q1/68

CPC classification number: C12Q1/6895 ,  C12Q2600/112 ,  C12Q2600/16

Abstract: The present technology provides methods and kits that may be used to detect and quantify the presence of Coccidioides species. The methods include quantification real-time PCR assays, and the kits and compositions include oligonucleotides used as primers and probes.

Abstract translation: 本技术提供了可用于检测和量化球孢子虫种类的存在的方法和试剂盒。 所述方法包括定量实时PCR测定，并且试剂盒和组合物包括用作引物和探针的寡核苷酸。

公开(公告)号：US20160143987A1

公开(公告)日：2016-05-26

申请号：US14949965

申请日：2015-11-24

Applicant: THE TRANSLATIONAL GENOMICS RESEARCH INSTITUTE ,  ARIZONA BOARD OF REGENTS ON BEHALF OF NORTHERN ARIZONA UNIVERSITY

Inventor： David Engelthaler ,  Elizabeth Driebe ,  Hongwei "Holly" Yin ,  Michael Valentine ,  Donald Chow ,  Jolene Bowers ,  Paul Keim

IPC: A61K38/13 ,  A61K31/58 ,  A61K31/4196 ,  A61K31/5377 ,  A61K31/5415 ,  A61K31/7135 ,  A61K33/24

CPC classification number: A61K38/13 ,  A61K31/138 ,  A61K31/277 ,  A61K31/353 ,  A61K31/404 ,  A61K31/4174 ,  A61K31/4196 ,  A61K31/428 ,  A61K31/4741 ,  A61K31/495 ,  A61K31/496 ,  A61K31/5377 ,  A61K31/5415 ,  A61K31/58 ,  A61K31/609 ,  A61K31/7135 ,  A61K45/06 ,  A61K2300/00

Abstract: The present invention comprises methods of treating an infection using a pharmaceutical composition comprising an active ingredient selected from Table 1. In some aspects, the infection can be caused by one or more pathogens, including fungal pathogens. For example, the infection may be Valley Fever.

Abstract translation: 本发明包括使用包含选自表1的活性成分的药物组合物治疗感染的方法。在一些方面，感染可以由一种或多种病原体引起，包括真菌病原体。 例如，感染可能是谷病。

公开(公告)号：US20220145375A1

公开(公告)日：2022-05-12

申请号：US17588196

申请日：2022-01-28

Applicant: THE TRANSLATIONAL GENOMICS RESEARCH INSTITUTE ,  ARIZONA BOARD OF REGENTS ON BEHALF OF NORTHERN ARIZONA UNIVERSITY

Inventor： Jolene Bowers ,  Elizabeth Driebe ,  David Engelthaler ,  Paul Keim ,  Darrin Lemmer

IPC: C12Q1/689 ,  C12Q1/6827 ,  C12Q1/6853 ,  C12Q1/686

Abstract: The present invention provides a method of detecting one or more Klebsiella species within a sample from a subject, the method comprising: subjecting DNA and/or RNA from the sample to a PCR amplification reaction using primer pairs targeting species-specific canonical single nucleotide polymorphisms (canSNPs); and analyzing amplification products resulting from the PCR amplification reaction to detect the one or more Klebsiella species. The present invention also provides a kit for detection of one or more Klebsiella species, Klebsiella clonal groups, AMR genes, and/or virulence genes, the kit comprising primer pairs targeting species-specific canSNPs, K. pneumoniae genes M1 and M2, clonal group-specific canSNPs, AMR genes, and/or virulence genes.