公开(公告)号：US12227753B2

公开(公告)日：2025-02-18

申请号：US16755534

申请日：2018-10-31

Applicant: The Regents of the University of California

Inventor： Jennifer A. Doudna ,  David Burstein ,  Janice S. Chen ,  Lucas B. Harrington ,  David Paez-Espino ,  Jillian F. Banfield

IPC: C12N15/82 ,  C12N9/22 ,  C12N15/113 ,  C12N15/85

Abstract: Provided are compositions and methods that include a CasY transactivating noncoding RNA (trancRNA) (referred to herein as a “CasY trancRNA”), nucleic acids encoding the CasY trancRNA, and/or a modified host cell comprising the CasY trancRNA (and/or a nucleic acid encoding the same). Subject compositions and methods can also include one or more of: (a) a “CasY” protein (also referred to as a CasY polypeptide, a Cas12d protein, and a Cas12d polypeptide), a nucleic acid encoding the CasY protein, and/or a modified host cell comprising the CasY protein (and/or a nucleic acid encoding the same); and (b) a CasY guide RNA (also referred to herein as a “Cas12d guide RNA”) that binds to and provides sequence specificity to the CasY protein, a nucleic acid encoding the CasY guide RNA, and/or a modified host cell comprising the CasY guide RNA (and/or a nucleic acid encoding the same).

公开(公告)号：US20200172886A1

公开(公告)日：2020-06-04

申请号：US16789049

申请日：2020-02-12

Applicant: The Regents of the University of California

Inventor： Jennifer A. Doudna ,  David Burstein ,  Janice S. Chen ,  Lucas B. Harrington ,  David Paez-Espino ,  Jillian F. Banfield

IPC: C12N9/22 ,  C12N15/113

Abstract: Provided are compositions and methods that include one or more of: (1) a “CasZ” protein (also referred to as a CasZ polypeptide), a nucleic acid encoding the CasZ protein, and/or a modified host cell comprising the CasZ protein (and/or a nucleic acid encoding the same); (2) a CasZ guide RNA that binds to and provides sequence specificity to the CasZ protein, a nucleic acid encoding the CasZ guide RNA, and/or a modified host cell comprising the CasZ guide RNA (and/or a nucleic acid encoding the same); and (3) a CasZ transactivating noncoding RNA (trancRNA) (referred to herein as a “CasZ trancRNA”), a nucleic acid encoding the CasZ trancRNA, and/or a modified host cell comprising the CasZ trancRNA (and/or a nucleic acid encoding the same).

公开(公告)号：US20240167009A1

公开(公告)日：2024-05-23

申请号：US18393303

申请日：2023-12-21

Applicant: The Regents of the University of California

Inventor： Jennifer A. Doudna ,  David Burstein ,  Janice S. Chen ,  Lucas B. Harrington ,  David Paez-Espino ,  Jillian F. Banfield

IPC: C12N9/22 ,  C12N15/11 ,  C12N15/113

CPC classification number: C12N9/22 ,  C12N15/11 ,  C12N15/113 ,  C07K2319/09 ,  C12N2310/20 ,  C12N2800/80

Abstract: Provided are compositions and methods that include one or more of: (1) a “CasZ” protein (also referred to as a CasZ polypeptide), a nucleic acid encoding the CasZ protein, and/or a modified host cell comprising the CasZ protein (and/or a nucleic acid encoding the same); (2) a CasZ guide RNA that binds to and provides sequence specificity to the CasZ protein, a nucleic acid encoding the CasZ guide RNA, and/or a modified host cell comprising the CasZ guide RNA (and/or a nucleic acid encoding the same); and (3) a CasZ transactivating noncoding RNA (trancRNA) (referred to herein as a “CasZ trancRNA”), a nucleic acid encoding the CasZ trancRNA, and/or a modified host cell comprising the CasZ trancRNA (and/or a nucleic acid encoding the same).

公开(公告)号：US11970719B2

公开(公告)日：2024-04-30

申请号：US16755535

申请日：2018-10-31

Applicant: The Regents of the University of California

Inventor： Jennifer A. Doudna ,  David Burstein ,  Janice S. Chen ,  Lucas B. Harrington ,  David Paez-Espino ,  Jillian F. Banfield

IPC: C12N9/22 ,  C12N15/11 ,  C12N15/82

CPC classification number: C12N9/22 ,  C12N15/11 ,  C12N15/8213 ,  C12N2310/20

Abstract: Provided are compositions and methods that include one or more of: (1) a Class 2 CRISPR/Cas effector protein, a nucleic acid encoding the effector protein, and/or a modified host cell comprising the effector protein (and/or a nucleic acid encoding the same); (2) a CRISPR/Cas guide RNA that binds to and provides sequence specificity to the Class 2 CRISPR/Cas effector protein, a nucleic acid encoding the CRISPR/Cas guide RNA, and/or a modified host cell comprising the CRISPR/Cas guide RNA (and/or a nucleic acid encoding the same); and (3) a CRISPR/Cas transactivating noncoding RNA (trancRNA), a nucleic acid encoding the CRISPR/Cas trancRNA, and/or a modified host cell comprising the CRISPR/Cas trancRNA (and/or a nucleic acid encoding the same).

公开(公告)号：US20230323319A1

公开(公告)日：2023-10-12

申请号：US17935521

申请日：2022-09-26

Applicant: The Regents of the University of California

Inventor： Jennifer A. Doudna ,  David Burstein ,  Janice S. Chen ,  Lucas B. Harrington ,  David Paez-Espino ,  Jillian F. Banfield

IPC: C12N9/22 ,  C12N15/11 ,  C12N15/113

CPC classification number: C12N9/22 ,  C12N15/11 ,  C12N15/113 ,  C12N2310/20 ,  C12N2800/80 ,  C07K2319/09

Abstract: Provided are compositions and methods that include one or more of: (1) a “CasZ” protein (also referred to as a CasZ polypeptide), a nucleic acid encoding the CasZ protein, and/or a modified host cell comprising the CasZ protein (and/or a nucleic acid encoding the same); (2) a CasZ guide RNA that binds to and provides sequence specificity to the CasZ protein, a nucleic acid encoding the CasZ guide RNA, and/or a modified host cell comprising the CasZ guide RNA (and/or a nucleic acid encoding the same); and (3) a CasZ transactivating noncoding RNA (trancRNA) (referred to herein as a “CasZ trancRNA”), a nucleic acid encoding the CasZ trancRNA, and/or a modified host cell comprising the CasZ trancRNA (and/or a nucleic acid encoding the same).

公开(公告)号：US11453866B2

公开(公告)日：2022-09-27

申请号：US16896711

申请日：2020-06-09

Applicant: The Regents of the University of California

Inventor： Jennifer A. Doudna ,  David Burstein ,  Janice S. Chen ,  Lucas B. Harrington ,  David Paez-Espino ,  Jillian F. Banfield

IPC: C12N9/22 ,  C12N15/11 ,  C12N15/113

Abstract: Provided are compositions and methods that include one or more of: (1) a “CasZ” protein (also referred to as a CasZ polypeptide), a nucleic acid encoding the CasZ protein, and/or a modified host cell comprising the CasZ protein (and/or a nucleic acid encoding the same); (2) a CasZ guide RNA that binds to and provides sequence specificity to the CasZ protein, a nucleic acid encoding the CasZ guide RNA, and/or a modified host cell comprising the CasZ guide RNA (and/or a nucleic acid encoding the same); and (3) a CasZ transactivating noncoding RNA (trancRNA) (referred to herein as a “CasZ trancRNA”), a nucleic acid encoding the CasZ trancRNA, and/or a modified host cell comprising the CasZ trancRNA (and/or a nucleic acid encoding the same).

公开(公告)号：US20180142222A1

公开(公告)日：2018-05-24

申请号：US15574748

申请日：2016-06-09

Applicant: The Regents of the University of California

Inventor： Samuel H. Sternberg ,  Jennifer A. Doudna ,  Benjamin LaFrance ,  Janice S. Chen

IPC: C12N9/22 ,  C12Q1/6876 ,  C12Q1/44

CPC classification number: C12N9/22 ,  C07K2319/60 ,  C12Q1/44 ,  C12Q1/6876 ,  C12Q1/6897 ,  G01N2333/922

Abstract: The present disclosure provides variant Cas9 proteins (e.g., reporter Cas9 proteins), nucleic acids encoding the variant Cas9 proteins, and host cells comprising the nucleic acids. The present disclosure provides systems and kits that include a subject variant Cas9 protein (e.g., reporter Cas9 proteins) (and/or a nucleic acid encoding the variant Cas9 protein). The variant Cas9 proteins (e.g., reporter Cas9 proteins) and the nucleic acids encoding the variant Cas9 proteins are useful in a wide variety of methods (including the detection of a conformational change of the variant Cas9 protein), which are also provided.

公开(公告)号：US20240301376A1

公开(公告)日：2024-09-12

申请号：US18618668

申请日：2024-03-27

Applicant: The Regents of the University of California

Inventor： Jennifer A. Doudna ,  David Burstein ,  Janice S. Chen ,  Lucas B. Harrington ,  David Paez-Espino ,  Jillian F. Banfield

IPC: C12N9/22 ,  C12N15/11 ,  C12N15/82

CPC classification number: C12N9/22 ,  C12N15/11 ,  C12N15/8213 ,  C12N2310/20

Abstract: Provided are compositions and methods that include one or more of: (1) a Class 2 CRISPR/Cas effector protein, a nucleic acid encoding the effector protein, and/or a modified host cell comprising the effector protein (and/or a nucleic acid encoding the same); (2) a CRISPR/Cas guide RNA that binds to and provides sequence specificity to the Class 2 CRISPR/Cas effector protein, a nucleic acid encoding the CRISPR/Cas guide RNA, and/or a modified host cell comprising the CRISPR/Cas guide RNA (and/or a nucleic acid encoding the same); and (3) a CRISPR/Cas transactivating noncoding RNA (trancRNA), a nucleic acid encoding the CRISPR/Cas trancRNA, and/or a modified host cell comprising the CRISPR/Cas trancRNA (and/or a nucleic acid encoding the same).

公开(公告)号：US11447824B2

公开(公告)日：2022-09-20

申请号：US16927351

申请日：2020-07-13

Applicant: The Regents of the University of California

Inventor： Jennifer A. Doudna ,  Janice S. Chen ,  Lucas Benjamin Harrington ,  Enbo Ma

IPC: C12Q1/6876 ,  C12N15/11 ,  C12N9/22 ,  C12Q1/6823

Abstract: Provided are compositions and methods for detecting a target DNA (double stranded or single stranded) in a sample. In some embodiments, a subject method includes: (a) contacting the sample with: (i) a type V CRISPR/Cas effector protein (e.g., a Cas12 protein such as Cas12a, Cas12b, Cas12c, Cas12d, Cas12e); (ii) a guide RNA (comprising a region that binds to the type V CRISPR/Cas effector protein, and a guide sequence that hybridizes with the target DNA); and (iii) a detector DNA that is single stranded (i.e., a “single stranded detector DNA”) and does not hybridize with the guide sequence of the guide RNA; and (b) measuring a detectable signal produced by cleavage (by the type V CRISPR/Cas effector protein) of the single stranded detector DNA. Also provided are compositions and methods for cleaving single stranded DNAs (e.g., non-target ssDNAs), e.g., inside of a cell.

公开(公告)号：US11441137B2

公开(公告)日：2022-09-13

申请号：US16884766

申请日：2020-05-27

Applicant: The Regents of the University of California

Inventor： Jennifer A. Doudna ,  David Burstein ,  Janice S. Chen ,  Lucas B. Harrington ,  David Paez-Espino ,  Jillian F. Banfield

IPC: C12Q1/6809 ,  C12N9/22 ,  C12N15/11 ,  C12N15/113

Abstract: Provided are compositions and methods that include one or more of: (1) a “CasZ” protein (also referred to as a CasZ polypeptide), a nucleic acid encoding the CasZ protein, and/or a modified host cell comprising the CasZ protein (and/or a nucleic acid encoding the same); (2) a CasZ guide RNA that binds to and provides sequence specificity to the CasZ protein, a nucleic acid encoding the CasZ guide RNA, and/or a modified host cell comprising the CasZ guide RNA (and/or a nucleic acid encoding the same); and (3) a CasZ transactivating noncoding RNA (trancRNA) (referred to herein as a “CasZ trancRNA”), a nucleic acid encoding the CasZ trancRNA, and/or a modified host cell comprising the CasZ trancRNA (and/or a nucleic acid encoding the same).