公开(公告)号：US20210348224A1

公开(公告)日：2021-11-11

申请号：US17056378

申请日：2019-05-22

Applicant: The Regents of the University of California

Inventor： Logan Mulroney ,  Mark Akeson ,  Miten Jain ,  Hugh Olsen

IPC: C12Q1/6869 ,  C12P19/34

Abstract: Provided are methods of analyzing capped ribonucleic acids (RNAs). The methods include translocating an adapted RNA through a nanopore of a nanopore device. The adapted RNA includes an RNA region, a 5′ cap, and an adapter polynucleotide attached to the 5′ cap. The methods include monitoring ionic current through the nanopore during the translocating, translocating the 5′ cap through the nanopore, and identifying one or more ionic current features characteristic of the 5′ cap (e.g., a triphosphate linkage between the 5′ cap and nucleotide N1 of the RNA region, a 5′ to 5′ orientation of the 5′ cap and nucleotide N1 of the RNA region, and/or the like), translocating through the nanopore. Also provided are computer-readable media, computer devices, and systems that find use, e.g., in practicing the methods of the present disclosure.

公开(公告)号：US12195794B2

公开(公告)日：2025-01-14

申请号：US17056378

申请日：2019-05-22

Applicant: The Regents of the University of California

Inventor： Logan Mulroney ,  Mark Akeson ,  Miten Jain ,  Hugh Olsen

IPC: C12Q1/6869 ,  C12P19/34

Abstract: Provided are methods of analyzing capped ribonucleic acids (RNAs). The methods include translocating an adapted RNA through a nanopore of a nanopore device. The adapted RNA includes an RNA region, a 5′ cap, and an adapter polynucleotide attached to the 5′ cap. The methods include monitoring ionic current through the nanopore during the translocating, translocating the 5′ cap through the nanopore, and identifying one or more ionic current features characteristic of the 5′ cap (e.g., a triphosphate linkage between the 5′ cap and nucleotide N1 of the RNA region, a 5′ to 5′ orientation of the 5′ cap and nucleotide N1 of the RNA region, and/or the like), translocating through the nanopore. Also provided are computer-readable media, computer devices, and systems that find use, e.g., in practicing the methods of the present disclosure.