公开(公告)号：US10788404B2

公开(公告)日：2020-09-29

申请号：US15935762

申请日：2018-03-26

申请人： The Florida State University Research Foundation, Inc.

发明人： Michael G. Roper ,  Nikita Mukhitov ,  Scott Stagg ,  John Spear

IPC分类号： G01N1/30 ,  G01N1/31 ,  H01J37/20

摘要： A sample preparation device for electron microscopy (EM) that is configured to eliminate user-to-user variations and environment contaminations, which are often present in the conventional method of sample preparation. The device not only provides a means for evenly and reproducibly delivering a fluid or sample to an EM grid, but also provides a means for sealing the EM grid in an air-tight chamber and delivering air-sensitive samples to the EM grid. The platform may comprise readily fabricated glass chips with features integrated to preserve the integrity of the sample grid and to facilitate its extraction. The methods may eliminate the element of user dependent variability and thus improve the throughput, reproducibility and translation of these methods.

公开(公告)号：US09958362B1

公开(公告)日：2018-05-01

申请号：US15263949

申请日：2016-09-13

申请人： The Florida State University Research Foundation, Inc.

发明人： Michael G. Roper ,  Nikita Mukhitov ,  Scott Stagg ,  John Spear

IPC分类号： G01N1/30 ,  G01N1/31 ,  H01J37/20

CPC分类号： G01N1/312 ,  G01N1/30 ,  H01J37/20 ,  H01J2237/2002 ,  H01J2237/2602

摘要： A sample preparation device for electron microscopy (EM) that is configured to eliminate user-to-user variations and environment contaminations, which are often present in the conventional method of sample preparation. The device not only provides a means for evenly and reproducibly delivering a fluid or sample to an EM grid, but also provides a means for sealing the EM grid in an air-tight chamber and delivering air-sensitive samples to the EM grid. The platform may comprise readily fabricated glass chips with features integrated to preserve the integrity of the sample grid and to facilitate its extraction. The methods may eliminate the element of user dependent variability and thus improve the throughput, reproducibility and translation of these methods.

公开(公告)号：US20180209880A1

公开(公告)日：2018-07-26

申请号：US15935762

申请日：2018-03-26

申请人： The Florida State University Research Foundation, Inc.

发明人： Michael G. Roper ,  Nikita Mukhitov ,  Scott Stagg ,  John Spear

IPC分类号： G01N1/31 ,  H01J37/20 ,  G01N1/30

CPC分类号： G01N1/312 ,  G01N1/30 ,  H01J37/20 ,  H01J2237/2002 ,  H01J2237/2602

摘要： A sample preparation device for electron microscopy (EM) that is configured to eliminate user-to-user variations and environment contaminations, which are often present in the conventional method of sample preparation. The device not only provides a means for evenly and reproducibly delivering a fluid or sample to an EM grid, but also provides a means for sealing the EM grid in an air-tight chamber and delivering air-sensitive samples to the EM grid. The platform may comprise readily fabricated glass chips with features integrated to preserve the integrity of the sample grid and to facilitate its extraction. The methods may eliminate the element of user dependent variability and thus improve the throughput, reproducibility and translation of these methods.