公开(公告)号：US20140014536A1

公开(公告)日：2014-01-16

申请号：US13941231

申请日：2013-07-12

申请人： THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

发明人： Gregory A. Weiss ,  Reginald M. Penner ,  Phillip Y. Tam ,  Li-Mei Yang ,  Tyler Brigham

IPC分类号： G01N27/327

CPC分类号： G01N27/327 ,  G01N27/3277

摘要： A biosensor that is capable of detecting the presence and/or concentration of an analyte or biomarker includes at least one electrically conductive electrode operatively coupled to an impedance analyzer for measuring the change in the resistive impedance ΔZRe of the electrode in response to an applied alternating current at a plurality of frequencies. In one embodiment, at least one electrode is covered with a self-assembled monolayer that is chemically bonded to a surface. A plurality of virus particles such as phage viruses are immobilized on the self-assembled monolayer and may be exposed to a test or sample solution. The virus particles may be obtained from phage-displayed libraries to detect a wide variety of targets including, for example, DNA, RNA, small molecules, and proteins or polypeptides. In another embodiment, the virus particles are electrostatically bound to a substrate in between a pair of elongated electrodes disposed on a substrate.

摘要翻译： 能够检测分析物或生物标志物的存在和/或浓度的生物传感器包括至少一个可操作地耦合到阻抗分析器的导电电极，用于响应于施加的交流电流来测量电极的电阻阻抗DeltaZRe的变化 在多个频率处。 在一个实施方案中，至少一个电极被化学键合到表面的自组装单层覆盖。 多个病毒颗粒如噬菌体病毒固定在自组装单层上，并可暴露于测试或样品溶液中。 病毒颗粒可以从噬菌体展示的文库中获得，以检测多种靶，包括例如DNA，RNA，小分子和蛋白质或多肽。 在另一个实施方案中，病毒颗粒在布置在基底上的一对细长电极之间静电结合到基底上。

公开(公告)号：US09316608B2

公开(公告)日：2016-04-19

申请号：US13941231

申请日：2013-07-12

申请人： THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

发明人： Gregory A. Weiss ,  Reginald M. Penner ,  Phillip Y. Tam ,  Li-Mei Yang ,  Tyler Brigham

IPC分类号： G01N27/327

CPC分类号： G01N27/327 ,  G01N27/3277

摘要： A biosensor that is capable of detecting the presence and/or concentration of an analyte or biomarker includes at least one electrically conductive electrode operatively coupled to an impedance analyzer for measuring the change in the resistive impedance ΔZRe of the electrode in response to an applied alternating current at a plurality of frequencies. In one embodiment, at least one electrode is covered with a self-assembled monolayer that is chemically bonded to a surface. A plurality of virus particles such as phage viruses are immobilized on the self-assembled monolayer and may be exposed to a test or sample solution. The virus particles may be obtained from phage-displayed libraries to detect a wide variety of targets including, for example, DNA, RNA, small molecules, and proteins or polypeptides. In another embodiment, the virus particles are electrostatically bound to a substrate in between a pair of elongated electrodes disposed on a substrate.

摘要翻译： 能够检测分析物或生物标志物的存在和/或浓度的生物传感器包括至少一个可操作地耦合到阻抗分析器的导电电极，用于测量响应于所施加的电极的电极的电阻阻抗和Dgr; Z Re的变化 在多个频率的交流电。 在一个实施方案中，至少一个电极被化学键合到表面的自组装单层覆盖。 多个病毒颗粒如噬菌体病毒固定在自组装单层上，并可暴露于测试或样品溶液中。 病毒颗粒可以从噬菌体展示的文库中获得，以检测多种靶，包括例如DNA，RNA，小分子和蛋白质或多肽。 在另一个实施方案中，病毒颗粒在布置在基底上的一对细长电极之间静电结合到基底上。