公开(公告)号：US11702688B2

公开(公告)日：2023-07-18

申请号：US16086144

申请日：2017-03-29

Applicant: SEKISUI MEDICAL CO., LTD.

Inventor： Hiroyuki Ebinuma ,  Katsura Uchida ,  Yuriko Tsukamoto

IPC: C12Q1/68 ,  C12Q1/6858 ,  C12N15/00 ,  C12N15/09 ,  C12Q1/6886 ,  C12Q1/6806 ,  C12Q1/6876

CPC classification number: C12Q1/6858 ,  C12N15/00 ,  C12N15/09 ,  C12Q1/68 ,  C12Q1/6806 ,  C12Q1/6876 ,  C12Q1/6886 ,  C12Q2600/156 ,  C12Y207/07007

Abstract: An object of the present invention is to provide a novel method for designing a primer ensuring reactivity and discriminatory power in a method for detecting a single base substitution based on an ASP-PCR method and to provide a method for easily detecting multiple point mutations within overlapping amplicons, particularly, two adjacent single base substitutions. The single base substitutions can easily be detected by using a mutant primer in which the base of the third nucleotide from the 3′ end corresponds to the base of a mutant nucleotide of a single base substitution contained in a nucleic acid sample, in which the base of the second nucleotide from the 3′ end is not complementary to the base of the corresponding nucleotide of the nucleic acid, and in which the bases of the other nucleotides are complementary to the bases of the corresponding nucleotides of the nucleic acid.