公开(公告)号：US10526536B2

公开(公告)日：2020-01-07

申请号：US16063660

申请日：2016-12-22

申请人： Roche Molecular Systems, Inc.

发明人： Dieter Heindl ,  Hannes Kirzinger ,  Thomas Meier ,  Christopher Nelson

IPC分类号： C12Q1/686 ,  C09K11/07 ,  C12Q1/6806

摘要： The present disclosure provides an aqueous composition comprising a purified thermostable polypeptide with biological activity, and a purified thermolabile water-soluble substance which in a functional conformation at a permissive temperature absorbs light and/or exhibits fluorescence, wherein the maximal permissive temperature of the polypeptide is above the maximal permissive temperature of the substance. The present disclosure further provides methods employing such a composition, and kits containing ingredients to form such a composition.

公开(公告)号：US20180371314A1

公开(公告)日：2018-12-27

申请号：US16063660

申请日：2016-12-22

申请人： Roche Molecular Systems, Inc.

发明人： Dieter Heindl ,  Hannes Kirzinger ,  Thomas Meier ,  Christopher Nelson

IPC分类号： C09K11/07 ,  C12Q1/686 ,  C12Q1/6806

CPC分类号： C09K11/07 ,  C12N9/1241 ,  C12Q1/68 ,  C12Q1/6806 ,  C12Q1/686 ,  C12Q2527/101 ,  C12Q2531/113 ,  C12Q2563/107 ,  C12Q2563/119 ,  C12Q2527/127

摘要： The present disclosure provides an aqueous composition comprising a purified thermostable polypeptide with biological activity, and a purified thermolabile water-soluble substance which in a functional conformation at a permissive temperature absorbs light and/or exhibits fluorescence, wherein the maximal permissive temperature of the polypeptide is above the maximal permissive temperature of the substance. The present disclosure further provides methods employing such a composition, and kits containing ingredients to form such a composition.