公开(公告)号：US12234472B2

公开(公告)日：2025-02-25

申请号：US18047341

申请日：2022-10-18

Applicant: Regeneron Pharmaceuticals, Inc.

Inventor： Michael Goren ,  Yu Zhao ,  Alexandros Strikoudis ,  Darya Burakov ,  Gang Chen

IPC: C12N15/85 ,  C07K14/005 ,  C12N15/67

Abstract: The present inventions provide eukaryotic cells, such as mammalian cells, that comprise adeno-associated virus (AAV) polynucleotides, including AAV capsid proteins (Cap), and are capable of expressing the polypeptides encoded by the AAV polynucleotides, and thereby are capable of producing AAV, including recombinant AAV. The eukaryotic cells also may comprise adenovirus (Ad) polynucleotides. The present inventions also provide methods of expressing AAV polynucleotides, as well as Ad polynucleotides, in eukaryotic cells, such as CHO cells, HEK 293 and BHK cells. The present inventions further provides other products and methods described herein.

公开(公告)号：US20230193312A1

公开(公告)日：2023-06-22

申请号：US18047341

申请日：2022-10-18

Applicant: Regeneron Pharmaceuticals, Inc.

Inventor： Michael Goren ,  Yu Zhao ,  Alexandros Strikoudis ,  Darya Burakov ,  Gang Chen

IPC: C12N15/85 ,  C07K14/005 ,  C12N15/67

CPC classification number: C12N15/85 ,  C07K14/005 ,  C12N15/67 ,  C12N2830/42 ,  C12N2830/50 ,  C12N2840/203

Abstract: The present inventions provide eukaryotic cells, such as mammalian cells, that comprise adeno-associated virus (AAV) polynucleotides, including AAV capsid proteins (Cap), and are capable of expressing the polypeptides encoded by the AAV polynucleotides, and thereby are capable of producing AAV, including recombinant AAV. The eukaryotic cells also may comprise adenovirus (Ad) polynucleotides. The present inventions also provide methods of expressing AAV polynucleotides, as well as Ad polynucleotides, in eukaryotic cells, such as CHO cells, HEK 293 and BHK cells. The present inventions further provides other products and methods described herein.

公开(公告)号：US11512144B2

公开(公告)日：2022-11-29

申请号：US16095084

申请日：2017-04-20

Applicant: REGENERON PHARMACEUTICALS, INC.

Inventor： Robert Babb ,  Darya Burakov ,  Gang Chen ,  James P. Fandl ,  Yu Zhao

IPC: C07K16/46 ,  C07K16/00 ,  C12N15/90 ,  C12N15/85

Abstract: This invention relates to site-specific integration and expression of recombinant proteins in eukaryotic cells. In particular, the invention includes compositions and methods for improved expression of antigen-binding proteins including monospecific and bispecifc antibodies in eukaryotic cells, particularly Chinese hamster (Cricetulus griseus) cell lines, by employing multiple expression-enhancing locus.

公开(公告)号：US20230304062A1

公开(公告)日：2023-09-28

申请号：US18047349

申请日：2022-10-18

Applicant: Regeneron Pharmaceuticals, Inc.

Inventor： Yu Zhao ,  Michael Goren ,  Darya Burakov ,  Gang Chen

IPC: C12P21/02 ,  C12N5/00 ,  C12N15/86

CPC classification number: C12P21/02 ,  C12N5/00 ,  C12N15/86 ,  C12N2750/14143 ,  C12N2830/002

Abstract: Compositions and methods for the transcription of polynucleotide sequences encoding polypeptides in cells are provided. The cells and methods provided are useful for controlling expression of polypeptides for a wide variety of purposes.

公开(公告)号：US20230287460A1

公开(公告)日：2023-09-14

申请号：US18047357

申请日：2022-10-18

Applicant: Regeneron Pharmaceuticals, Inc.

Inventor： Michael Goren ,  Darya Burakov ,  Gang Chen ,  Yu Zhao ,  Dipali Deshpande

IPC: C12N15/90 ,  C12N5/00 ,  C12N15/11 ,  C12N9/22

CPC classification number: C12N15/907 ,  C12N5/00 ,  C12N15/11 ,  C12N9/22 ,  C12N2310/20 ,  C12N2800/80 ,  C12N2820/002

Abstract: The present inventions provide mammalian cells that comprise multiple Stable Integration Sites. The inventions provide sites introduced genomically into a Genomic Safe Harbor and introduced genomically outside of that particular Genomic Safe Harbor, including but not limited to another Genomic Safe Harbor. Polynucleotides of interest that encode polypeptides or RNAs of interest can be inserted into the Stable Integration Sites provided according to the inventions. The cells and methods of the inventions can be used for the high yield production of any protein, including viral proteins. Additionally, the cells and methods of the inventions are useful for production of viral vectors, such as AAV, antibodies and other proteins.