公开(公告)号：US12043863B2

公开(公告)日：2024-07-23

申请号：US17400168

申请日：2021-08-12

Applicant: Ramot at Tel-Aviv University Ltd.

Inventor： Yuval Ebenstein ,  Micha Fridman

IPC: C12Q1/6806 ,  C12P19/30 ,  G01N21/64

CPC classification number: C12Q1/6806 ,  C12P19/305 ,  G01N21/6428 ,  C12Q1/6827 ,  C12Q2537/164

Abstract: Provided herein is a method of labeling the epigenetic modification 5-hydroxymethyl-cytosine along a DNA molecule that involves attaching a 5-hydroxymethyl-cytosine specific labeling agent to the DNA molecule, which is effected by enzymatically synthesizing UDP-6-N3-Glucose by subjecting 6-azidoglucose to an enzymatic catalysis by N-acetylhexoseamine 1-kinase (NahK) in the presence of ATP to thereby obtain a phosphorylated 6-azidoglucose, followed by subjecting the phosphorylated 6-azidoglucose to enzymatic catalysis by uridyltransferase (GlmU) in the presence of UTP to thereby obtain UDP-6-N3-Glucose, followed by incubating the DNA molecule with UDP-6-N3-Glucose and ß-glucosyltransferase, to thereby obtain a 5-hydroxymethyl-cytosin e glycosylated by a 6-azidoglucose residue in the DNA molecule, and reacting the modified DNA molecule with a labeling agent which is chemically compatible with the azide group of the 6-azidoglucose residue.

公开(公告)号：US10351898B2

公开(公告)日：2019-07-16

申请号：US14894350

申请日：2014-01-05

Applicant: Ramot at Tel-Aviv University Ltd.

Inventor： Yuval Ebenstein ,  Micha Fridman

IPC: C12Q1/6827 ,  C12Q1/6806 ,  C12P19/30 ,  G01N21/64

Abstract: Methodologies for labeling the epigenetic modification 5-hydroxymethyl-cytosine (5hmC) along a DNA molecule, and for imaging this epigenetic modification along a DNA molecule are disclosed. Related compositions and reagents, and methods of preparing same are also disclosed.

公开(公告)号：US20220119339A1

公开(公告)日：2022-04-21

申请号：US17452970

申请日：2021-10-29

Applicant: RAMOT AT TEL-AVIV UNIVERSITY LTD.

Inventor： Yoram Cohen ,  Micha Fridman ,  Roymon Joseph ,  Alissa Naugolny ,  Mark Feldman ,  Ido M. Herzog ,  Dana Kaizerman

IPC: C07C217/54 ,  A61K31/138 ,  A61K31/4178 ,  A61K31/66 ,  A61K45/06 ,  C02F1/50 ,  C07D233/60 ,  C07F9/54 ,  C09D5/16

Abstract: Cationic pillar[n]arenes, e.g., positively charged poly-ammonium, poly-phosphonium and poly-imidazolium pillar[5-6]arene derivatives are capable of inhibiting or preventing biofilm formation, and facilitating existing biofilm decomposition. A composition for inhibiting or disrupting biofilm, e.g., bacterial or fungal biofilm, formation, or reducing biofilm, can include a pharmaceutically acceptable carrier, and a cationic pillar[n]ene.

公开(公告)号：US20200172471A1

公开(公告)日：2020-06-04

申请号：US15751786

申请日：2016-08-03

Applicant: RAMOT AT TEL-AVIV UNIVERSITY LTD.

Inventor： Yoram Cohen ,  Micha Fridman ,  Roymon Joseph ,  Alissa Naugolny ,  Mark Feldman ,  Ido M. Herzog ,  Dana Kaizerman

IPC: C07C217/54 ,  C09D5/16 ,  A61K45/06 ,  A61K31/138 ,  C07D233/60 ,  C07F9/54 ,  A61K31/4178 ,  A61K31/66 ,  C02F1/50

Abstract: The present invention provides cationic pillar[n]arenes, e.g., positively charged poly-ammonium, poly-phosphonium and poly-imidazolium pillar[5-6]arene derivatives, capable of inhibiting or preventing biofilm formation, and facilitating existing biofilm decomposition; as well as compositions thereof and methods of use.

公开(公告)号：US20230365491A1

公开(公告)日：2023-11-16

申请号：US18352955

申请日：2023-07-14

Applicant: RAMOT AT TEL-AVIV UNIVERSITY LTD.

Inventor： Yoram Cohen ,  Micha Fridman ,  Roymon Joseph ,  Alissa Naugolny ,  Mark Feldman ,  Ido M. Herzog ,  Dana Kaizerman

IPC: C07C217/54 ,  A61K31/138 ,  A61K31/4178 ,  A61K31/66 ,  A61K45/06 ,  C02F1/50 ,  C07D233/60 ,  C07F9/54 ,  C09D5/16

CPC classification number: C07C217/54 ,  A61K31/138 ,  A61K31/4178 ,  A61K31/66 ,  A61K45/06 ,  C02F1/50 ,  C07D233/60 ,  C07F9/5407 ,  C09D5/1687 ,  C02F2303/20

Abstract: Cationic pillar[n]arenes, e.g., positively charged poly-ammonium, poly-phosphonium and poly-imidazolium pillar[5-6]arene derivatives are capable of inhibiting or preventing biofilm formation, and facilitating existing biofilm decomposition. A composition for inhibiting or disrupting biofilm, e.g., bacterial or fungal biofilm, formation, or reducing biofilm, can include a pharmaceutically acceptable carrier, and a cationic pillar[n]ene.

公开(公告)号：US11161801B2

公开(公告)日：2021-11-02

申请号：US15751786

申请日：2016-08-03

Applicant: RAMOT AT TEL-AVIV UNIVERSITY LTD.

Inventor： Yoram Cohen ,  Micha Fridman ,  Roymon Joseph ,  Alissa Naugolny ,  Mark Feldman ,  Ido M. Herzog ,  Dana Kaizerman

IPC: C02F1/50 ,  C07C217/54 ,  C07D233/60 ,  C07F9/54 ,  C09D5/16 ,  A61K31/138 ,  A61K31/4178 ,  A61K31/66 ,  A61K45/06

Abstract: The present invention provides cationic pillar[n]arenes, e.g., positively charged poly-ammonium, poly-phosphonium and poly-imidazolium pillar[5-6]arene derivatives, capable of inhibiting or preventing biofilm formation, and facilitating existing biofilm decomposition; as well as compositions thereof and methods of use.

公开(公告)号：US11091793B2

公开(公告)日：2021-08-17

申请号：US16424523

申请日：2019-05-29

Applicant: Ramot at Tel-Aviv University Ltd.

Inventor： Yuval Ebenstein ,  Micha Fridman

IPC: C12P19/30 ,  C12Q1/6806 ,  G01N21/64

Abstract: Methodologies for labeling the epigenetic modification 5-hydroxymethylcytosine (5hmC) along a DNA molecule, and for determining a presence or a level of this epigenetic modification based on a ratio of fluorescence intensity of a labeled DNA sample to absorption intensity of the DNA sample at 260 nm are disclosed. Related compositions and reagents, and methods of preparing same are also disclosed.

公开(公告)号：US20200072711A1

公开(公告)日：2020-03-05

申请号：US16554360

申请日：2019-08-28

Applicant: Ramot at Tel Aviv University Ltd.

Inventor： Micha Fridman ,  Raphael I. Benhamou ,  Ido M. Herzog ,  Qais Z. Jaber

IPC: G01N1/30 ,  G01N33/569 ,  G01N33/58 ,  C09K11/06 ,  C09B62/343

Abstract: Particular 1,2,3-triazole- and 1,2,4-triazole-based compounds can be used as fluorescent probes. The compounds can optionally be substituted with one or two fluorine atoms. The compounds are effective as specific endoplasmatic reticulum (ER)-trackers for live cell imaging in a fungal cell, such as a pathogenic yeast. Compositions including the fluorescent probes can be used in methods for tracking the endoplasmic reticulum in a fungal cell.