公开(公告)号：US20170204450A1

公开(公告)日：2017-07-20

申请号：US15312780

申请日：2015-05-28

申请人： RUPRECHT-KARLS-UNIVERSITAET HEIDELBERG

发明人： Stefan KRIENKE ,  Michael HUNDEMER

IPC分类号： C12Q1/37 ,  G01N33/50 ,  C12N9/52 ,  G01N15/14 ,  C12N9/76 ,  C12N9/64

CPC分类号： C12Q1/37 ,  C12N9/50 ,  C12N9/52 ,  C12N9/6416 ,  C12N9/6427 ,  C12Y304/21004 ,  C12Y304/24021 ,  C12Y304/24024 ,  G01N15/1404 ,  G01N33/5047 ,  G01N2333/96433 ,  G01N2333/96486

摘要： The invention relates to an enzyme solution, which is used preferably for pre-treating mammalian cells for flow-cytometric detection of light chain restriction of the cells, the enzyme solution comprising at least two proteases, which are proteolytic and also collagenolytic, in a buffer. Preferably, the enzyme solution comprises trypsin, collagenase 4, dispase and astacin. The invention also relates to uses of the enzyme solution and methods for flow-cytometric detection of light chain restriction of cells.