公开(公告)号：US10150984B2

公开(公告)日：2018-12-11

申请号：US15358320

申请日：2016-11-22

Applicant: Purdue Research Foundation

Inventor： Laurie L Parker ,  Andrew Michael Lipchik ,  Scott Charles Bolton

IPC: C12Q1/48

Abstract: Disclosed are compositions and methods for measuring tyrosine kinase activity.

公开(公告)号：US09499854B2

公开(公告)日：2016-11-22

申请号：US13761968

申请日：2013-02-07

Applicant: Purdue Research Foundation

Inventor： Laurie Louise Parker ,  Andrew M. Lipchik ,  Scott Charles Bolton

IPC: C12Q1/48

CPC classification number: C12Q1/485

Abstract: The claims describe a process for designing and validating peptide-based substrates for a specified kinase. The process uses a rational approach to design peptides with a specific amino acid that is phosphorylated by a target tyrosine kinase, and upon phosphorylation shows an increased affinity for a lanthanide ion that results in an enhanced fluorescent signal. The process uses kinase specific input data sets, encodes the input into positional scoring matrices, analyses the positional scoring matrices in order to generate a candidate list of peptide sequences, compares the peptide sequences in the candidate list, substitutes residues at non-selective positions in selections from the candidate list with terbium binding residues, and then screens the substituted selections with different kinases in vitro.

Abstract translation: 权利要求描述了用于设计和验证特定激酶的基于肽的底物的方法。 该方法使用合理的方法来设计具有由靶酪氨酸激酶磷酸化的特定氨基酸的肽，并且在磷酸化时显示增加的对镧系元素离子的亲和力，其导致增强的荧光信号。 该过程使用激酶特异性输入数据集，将输入编码为位置评分矩阵，分析位置评分矩阵以产生肽序列的候选列表，比较候选列表中的肽序列，替代候选列表中的非选择位置的残基 从候选列表中选择铽结合残基，然后在体外筛选不同激酶的取代选择。