公开(公告)号：US20160279231A1

公开(公告)日：2016-09-29

申请号：US15030345

申请日：2015-07-06

申请人： Pulike Biological Engineering, Inc.

发明人： Kegong Tian ,  Feifei Tan ,  Jinzhong Sun ,  Xuke Zhang

IPC分类号： A61K39/245 ,  C12N7/00

CPC分类号： A61K39/245 ,  A61K39/12 ,  A61K2039/5254 ,  C12N7/00 ,  C12N7/08 ,  C12N2710/16734 ,  C12N2710/16764

摘要： This invention provides a method of attenuating porcine pseudorabies virus, which can effectively and reproducibly attenuate porcine pseudorabies virus. The attenuated strain of porcine pseudorabies virus by use of said method can provide effective immunization for pigs.

摘要翻译： 本发明提供了一种减轻猪伪狂犬病病毒的方法，可有效和可重复地减弱猪伪狂犬病病毒。 通过使用所述方法，猪伪狂犬病病毒的减毒菌株可以为猪提供有效的免疫。

公开(公告)号：US10201605B2

公开(公告)日：2019-02-12

申请号：US15213662

申请日：2016-07-19

申请人： Pulike Biological Engineering, Inc.

发明人： Kegong Tian ,  Xuke Zhang ,  Jinzhong Sun ,  Feifei Tan

IPC分类号： A61K39/00 ,  C12N7/00 ,  A61K39/245 ,  C12N7/04 ,  A61K39/12 ,  A61K45/06

摘要： The present invention provides an attenuated strain of porcine pseudorabies virus (PRV), in which said attenuated strain of PRV is a variant strain of PRV with inactivation of gI/gE/11K/28K proteins. In addition, the present invention also provides a vaccine composition comprising the attenuated strain of PRV as an antigen, a preparation method and use thereof. Proved by immunogenicity and pathogenicity testing of the live vaccine, said live PRV vaccine can provide a good protection for pigs with no clinical signs observed, indicating excellent immune protection.

公开(公告)号：US10548972B2

公开(公告)日：2020-02-04

申请号：US14901981

申请日：2015-01-06

申请人： Pulike Biological Engineering, Inc.

发明人： Kegong Tian ,  Xuke Zhang ,  Jinzhong Sun ,  Feifei Tan

IPC分类号： C12N7/00 ,  A61K39/00 ,  A61K39/245 ,  C12N7/04 ,  A61K39/12 ,  A61K45/06

摘要： The present invention provides an attenuated strain of porcine pseudorabies virus (PRV), in which said attenuated strain of PRV is a variant strain of PRV with inactivation of gI/gE/11K/28K proteins. In addition, the present invention also provides a vaccine composition comprising the attenuated strain of PRV as an antigen, a preparation method and use thereof. Proved by immunogenicity and pathogenicity testing of the live vaccine, said live PRV vaccine can provide a good protection for pigs with no clinical signs observed, indicating excellent immune protection.

公开(公告)号：US20160317651A1

公开(公告)日：2016-11-03

申请号：US15213711

申请日：2016-07-19

申请人： Pulike Biological Engineering, Inc.

发明人： Kegong Tian ,  Feifei Tan ,  Jinzhong Sun ,  Xuke Zhang

IPC分类号： A61K39/245 ,  C12N7/00

CPC分类号： A61K39/245 ,  A61K39/12 ,  A61K2039/5254 ,  C12N7/00 ,  C12N7/08 ,  C12N2710/16734 ,  C12N2710/16764

摘要： This invention provides a method of attenuating porcine pseudorabies virus, which can effectively and reproducibly attenuate porcine pseudorabies virus. The attenuated strain of porcine pseudorabies virus by use of said method can provide effective immunization for pigs.

摘要翻译： 本发明提供了一种减轻猪伪狂犬病病毒的方法，可有效和可重复地减弱猪伪狂犬病病毒。 通过使用所述方法，猪伪狂犬病病毒的减毒菌株可以为猪提供有效的免疫。

公开(公告)号：US09795667B2

公开(公告)日：2017-10-24

申请号：US15030345

申请日：2015-07-06

申请人： Pulike Biological Engineering, Inc.

发明人： Kegong Tian ,  Feifei Tan ,  Jinzhong Sun ,  Xuke Zhang

IPC分类号： C12N7/04 ,  A61K39/00 ,  A61K39/245 ,  C12N7/08 ,  C12N7/00 ,  A61K39/12

CPC分类号： A61K39/245 ,  A61K39/12 ,  A61K2039/5254 ,  C12N7/00 ,  C12N7/08 ,  C12N2710/16734 ,  C12N2710/16764

摘要： This invention provides a method of attenuating porcine pseudorabies virus, which can effectively and reproducibly attenuate porcine pseudorabies virus. The attenuated strain of porcine pseudorabies virus by use of said method can provide effective immunization for pigs.

公开(公告)号：US09801936B2

公开(公告)日：2017-10-31

申请号：US15213711

申请日：2016-07-19

申请人： Pulike Biological Engineering, Inc.

发明人： Kegong Tian ,  Feifei Tan ,  Jinzhong Sun ,  Xuke Zhang

IPC分类号： A61K39/00 ,  C12N7/00 ,  A61K39/245 ,  C12N7/08 ,  A61K39/12

CPC分类号： A61K39/245 ,  A61K39/12 ,  A61K2039/5254 ,  C12N7/00 ,  C12N7/08 ,  C12N2710/16734 ,  C12N2710/16764

摘要： This invention provides a method of attenuating porcine pseudorabies virus, which can effectively and reproducibly attenuate porcine pseudorabies virus. The attenuated strain of porcine pseudorabies virus by use of said method can provide effective immunization for pigs.

公开(公告)号：US09650424B2

公开(公告)日：2017-05-16

申请号：US14980673

申请日：2015-12-28

申请人： PULIKE BIOLOGICAL ENGINEERING, INC.

发明人： Xuke Zhang ,  Jinzhong Sun ,  Rui Wu ,  Feifei Tan ,  Chaoyong Bai ,  Kegong Tian

IPC分类号： A61K39/205 ,  C12N7/00 ,  C07K14/005 ,  C07H21/04 ,  C12N7/04 ,  A61K39/12 ,  A61K39/245 ,  A61K39/00

CPC分类号： C07K14/005 ,  A61K39/00 ,  A61K39/12 ,  A61K39/245 ,  A61K2039/5252 ,  A61K2039/552 ,  C07H21/04 ,  C12N7/00 ,  C12N7/04 ,  C12N2710/16721 ,  C12N2710/16722 ,  C12N2710/16734 ,  C12N2710/16761 ,  C12N2710/16771

摘要： Provided in the embodiments of the present invention is a vaccine composition including an immune amount of attenuated live vaccine, inactivated vaccine, subunit vaccine, synthetic vaccine, or genetically engineered vaccine of the porcine pseudorabies virus strain. The vaccine composition can effectively induce antibody generation, and prevent infections of virulent strains of the porcine pseudorabies virus, and provides effective protection for pigs.