公开(公告)号：US20230303968A1

公开(公告)日：2023-09-28

申请号：US17993674

申请日：2022-11-23

Applicant: President and Fellows of Harvard College

Inventor： Torsten B. Meissner ,  Leonardo M.R. Ferreira ,  Jack L. Strominger ,  Chad A. Cowan

IPC: C12N5/0735 ,  C12N5/074 ,  C12N15/86 ,  C12N15/90 ,  A61K39/00

CPC classification number: C12N5/0606 ,  C12N5/0696 ,  C12N15/86 ,  C12N15/907 ,  A61K39/001 ,  C12N2310/20 ,  C12N2501/599 ,  C12N2501/998 ,  C12N2510/00 ,  C12N2501/50

Abstract: Disclosed herein are universal donor stem cells and related methods of their use and production. The universal donor stem cells disclosed herein are useful for overcoming the immune rejection in cell-based transplantation therapies. In certain embodiments, the universal donor stem cells disclosed herein do not express one or more MHC-I and MHC-II human leukocyte antigens. Similarly, in certain embodiments, the universal donor stem cells disclosed herein do not express one or more human leukocyte antigens (e.g., HLA-A, HLA-B and/or HLA-C) corresponding to MHC-I and MHC-II human leukocyte antigens, thereby rendering such cells hypoimmunogenic.

公开(公告)号：US20220162555A1

公开(公告)日：2022-05-26

申请号：US17540968

申请日：2021-12-02

Applicant: President and Fellows of Harvard College

Inventor： Torsten B. Meissner ,  Kabungo Y. Mulumba ,  Leonardo M.R. Ferreira ,  Chad A. Cowan

IPC: C12N5/0783 ,  C12N15/113 ,  A61K35/17 ,  C12N15/10 ,  C12N15/85

Abstract: Disclosed herein are modified primary human T cells and populations thereof comprising a genome in which the CTLA4, PD1, TCRA, TCRB, and/or B2M genes have been edited to generate an off-the-shelf universal CAR T cell from allogeneic healthy donors that can be administered to any patient while reducing or eliminating the risk of immune rejection or graft versus host disease, and which are not prone to T cell inhibition, and methods for allogeneic administration of such cells to reduce the likelihood that the cells will trigger a host immune response when the cells are administered to a subject in need of such cells.

公开(公告)号：US20240141288A1

公开(公告)日：2024-05-02

申请号：US17993659

申请日：2022-11-23

Applicant: President and Fellows of Harvard College

Inventor： Torsten B. Meissner ,  Leonardo M.R. Ferreira ,  Jack L. Strominger ,  Chad A. Cowan

IPC: C12N5/0735 ,  A61K39/00 ,  C12N5/074 ,  C12N15/86 ,  C12N15/90

CPC classification number: C12N5/0606 ,  A61K39/001 ,  C12N5/0696 ,  C12N15/86 ,  C12N15/907 ,  C12N2310/20 ,  C12N2501/50 ,  C12N2501/599 ,  C12N2501/998 ,  C12N2510/00

Abstract: Disclosed herein are universal donor stem cells and related methods of their use and production. The universal donor stem cells disclosed herein are useful for overcoming the immune rejection in cell-based transplantation therapies. In certain embodiments, the universal donor stem cells disclosed herein do not express one or more MHC-I and MHC-II human leukocyte antigens. Similarly, in certain embodiments, the universal donor stem cells disclosed herein do not express one or more human leukocyte antigens (e.g., HLA-A, HLA-B and/or HLA-C) corresponding to MHC-I and MHC-II human leukocyte antigens, thereby rendering such cells hypoimmunogenic.

公开(公告)号：US20220162554A1

公开(公告)日：2022-05-26

申请号：US17525644

申请日：2021-11-12

Applicant: President and Fellows of Harvard College

Inventor： Torsten B. Meissner ,  Kabungo Y. Mulumba ,  Leonardo M.R. Ferreira ,  Chad A. Cowan

IPC: C12N5/0783 ,  A61K35/17 ,  C12N15/113 ,  C12N15/10 ,  C12N15/85

Abstract: Disclosed herein are modified primary human T cells and populations thereof comprising a genome in which the CTLA4, PD1, TCRA, TCRB, and/or B2M genes have been edited to generate an off-the-shelf universal CAR T cell from allogeneic healthy donors that can be administered to any patient while reducing or eliminating the risk of immune rejection or graft versus host disease, and which are not prone to T cell inhibition, and methods for allogeneic administration of such cells to reduce the likelihood that the cells will trigger a host immune response when the cells are administered to a subject in need of such cells.

公开(公告)号：US20240368540A1

公开(公告)日：2024-11-07

申请号：US18658385

申请日：2024-05-08

Applicant: President and Fellows of Harvard College

Inventor： Torsten B. Meissner ,  Leonardo M.R. Ferreira ,  Jack L. Strominger ,  Chad A. Cowan

IPC: C12N5/0735 ,  A61K39/00 ,  C12N5/074 ,  C12N15/86 ,  C12N15/90

Abstract: Disclosed herein are universal donor stem cells and related methods of their use and production. The universal donor stem cells disclosed herein are useful for overcoming the immune rejection in cell-based transplantation therapies. In certain embodiments, the universal donor stem cells disclosed herein do not express one or more MHC-I and MHC-II human leukocyte antigens. Similarly, in certain embodiments, the universal donor stem cells disclosed herein do not express one or more human leukocyte antigens (e.g., HLA-A, HLA-B and/or HLA-C) corresponding to MHC-I and MHC-II human leukocyte antigens, thereby rendering such cells hypoimmunogenic.

公开(公告)号：US20230323288A1

公开(公告)日：2023-10-12

申请号：US17993667

申请日：2022-11-23

Applicant: President and Fellows of Harvard College

Inventor： Torsten B. Meissner ,  Leonardo M.R. Ferreira ,  Jack L. Strominger ,  Chad A. Cowan

IPC: C12N15/86 ,  C12N5/0735 ,  C12N5/074 ,  C12N15/90 ,  A61K39/00

CPC classification number: C12N5/0606 ,  A61K39/001 ,  C12N5/0696 ,  C12N15/86 ,  C12N15/907 ,  C12N2310/20 ,  C12N2501/50 ,  C12N2501/599 ,  C12N2501/998 ,  C12N2510/00

Abstract: Disclosed herein are universal donor stem cells and related methods of their use and production. The universal donor stem cells disclosed herein are useful for overcoming the immune rejection in cell-based transplantation therapies. In certain embodiments, the universal donor stem cells disclosed herein do not express one or more MHC-I and MHC-II human leukocyte antigens. Similarly, in certain embodiments, the universal donor stem cells disclosed herein do not express one or more human leukocyte antigens (e.g., HLA-A, HLA-B and/or HLA-C) corresponding to MHC-I and MHC-II human leukocyte antigens, thereby rendering such cells hypoimmunogenic.

公开(公告)号：US20220112459A1

公开(公告)日：2022-04-14

申请号：US17497927

申请日：2021-10-09

Applicant: President and Fellows of Harvard College

Inventor： Torsten B. Meissner ,  Leonardo M.R. Ferreira ,  Jack L. Strominger ,  Chad A. Cowan

IPC: C12N5/0735 ,  C12N5/074 ,  C12N15/86 ,  C12N15/90 ,  A61K39/00

Abstract: Disclosed herein are universal donor stem cells and related methods of their use and production. The universal donor stem cells disclosed herein are useful for overcoming the immune rejection in cell-based transplantation therapies. In certain embodiments, the universal donor stem cells disclosed herein do not express one or more MHC-I and MHC-II human leukocyte antigens. Similarly, in certain embodiments, the universal donor stem cells disclosed herein do not express one or more human leukocyte antigens (e.g., HLA-A, HLA-B and/or HLA-C) corresponding to MHC-I and MHC-II human leukocyte antigens, thereby rendering such cells hypoimmunogenic.

公开(公告)号：US20240368539A1

公开(公告)日：2024-11-07

申请号：US18658343

申请日：2024-05-08

Applicant: President and Fellows of Harvard College

Inventor： Torsten B. Meissner ,  Leonardo M.R. Ferreira ,  Jack L. Strominger ,  Chad A. Cowan

IPC: C12N5/0735 ,  A61K39/00 ,  C12N5/074 ,  C12N15/86 ,  C12N15/90

Abstract: Disclosed herein are universal donor stem cells and related methods of their use and production. The universal donor stem cells disclosed herein are useful for overcoming the immune rejection in cell-based transplantation therapies. In certain embodiments, the universal donor stem cells disclosed herein do not express one or more MHC-I and MHC-II human leukocyte antigens. Similarly, in certain embodiments, the universal donor stem cells disclosed herein do not express one or more human leukocyte antigens (e.g., HLA-A, HLA-B and/or HLA-C) corresponding to MHC-I and MHC-II human leukocyte antigens, thereby rendering such cells hypoimmunogenic.

公开(公告)号：US20210171903A1

公开(公告)日：2021-06-10

申请号：US16908618

申请日：2020-06-22

Applicant: President and Fellows of Harvard College

Inventor： Torsten B. Meissner ,  Leonardo M.R. Ferreira ,  Jack L. Strominger ,  Chad A. Cowan

IPC: C12N5/0735 ,  C07K14/705 ,  C07K14/74

Abstract: Disclosed herein are universal donor stem cells and related methods of their use and production. The universal donor stem cells disclosed herein are useful for overcoming the immune rejection in cell-based transplantation therapies. In certain embodiments, the universal donor stem cells disclosed herein have modulated expression of one or more MHC-I and MHC-II human leukocyte antigens and one or more tolerogenic factors.

公开(公告)号：US20190309259A1

公开(公告)日：2019-10-10

申请号：US15572776

申请日：2016-05-09

Applicant: President and Fellows of Harvard College

Inventor： Torsten B. Meissner ,  Leonardo M.R. Ferreira ,  Jack L. Strominger ,  Chad A. Cowan

IPC: C12N5/0735 ,  C12N5/074 ,  C12N15/86 ,  C12N15/90

Abstract: Disclosed herein are universal donor stem cells and related methods of their use and production. The universal donor stem cells disclosed herein are useful for overcoming the immune rejection in cell-based transplantation therapies. In certain embodiments, the universal donor stem cells disclosed herein do not express one or more MHC-I and MHC-II human leukocyte antigens. Similarly, in certain embodiments, the universal donor stem cells disclosed herein do not express one or more human leukocyte antigens (e.g., HLA-A, HLA-B and/or HLA-C) corresponding to MHC-I and MHC-II human leukocyte antigens, thereby rendering such cells hypoimmunogenic.