公开(公告)号：US20140209814A1

公开(公告)日：2014-07-31

申请号：US14229489

申请日：2014-03-28

申请人： PerkinElmer Health Sciences, Inc.

发明人： Craig M. Whitehouse ,  David G. Welkie ,  Gholamreza Javahery ,  Lisa Cousins ,  Sergey Rakov

IPC分类号： H01J49/14

CPC分类号： H01J49/147 ,  H01J49/0054

摘要： Apparatus and methods are provided that enable the interaction of low-energy electrons and positrons with sample ions to facilitate electron capture dissociation (ECD) and positron capture dissociation (PCD), respectively, within multipole ion guide structures. It has recently been discovered that fragmentation of protonated ions of many biomolecules via ECD often proceeds along fragmentation pathways not accessed by other dissociation methods, leading to molecular structure information not otherwise easily obtainable. However, such analyses have been limited to expensive Fourier transform ion cyclotron resonance (FTICR) mass spectrometers; the implementation of ECD within commonly-used multipole ion guide structures is problematic due to the disturbing effects of RF fields within such devices. The apparatus and methods described herein successfully overcome such difficulties, and allow ECD (and PCD) to be performed within multipole ion guides, either alone, or in combination with conventional ion fragmentation methods. Therefore, improved analytical performance and functionality of mass spectrometers that utilize multipole ion guides are provided.

摘要翻译： 提供了能够使低能电子和正电子与样品离子相互作用的装置和方法，以便分别在多极离子导向结构内的电子俘获解离（ECD）和正电子俘获解离（PCD）。 最近已经发现，许多生物分子通过ECD的质子化离子的碎裂通常沿着不被其它解离方法接近的断裂途径进行，导致分子结构信息不容易获得。 然而，这种分析仅限于昂贵的傅里叶变换离子回旋共振（FTICR）质谱仪; 在常用的多极离子导向结构中的ECD的实现是由于RF场在这种装置内的干扰效应而成问题的。 本文描述的装置和方法成功地克服了这些困难，并且允许ECD（和PCD）在多极离子导向器中单独进行，或者与传统的离子碎裂方法组合进行。 因此，提供了利用多极离子导向器的质谱仪的改进的分析性能和功能性。

公开(公告)号：US08981290B2

公开(公告)日：2015-03-17

申请号：US14229489

申请日：2014-03-28

申请人： PerkinElmer Health Sciences, Inc.

发明人： Craig M. Whitehouse ,  David G. Welkie ,  Gholamreza Javahery ,  Lisa Cousins ,  Sergey Rakov

IPC分类号： H01J49/42 ,  H01J49/26 ,  B01D59/44 ,  H01J49/14 ,  H01J49/00

CPC分类号： H01J49/147 ,  H01J49/0054

摘要： Apparatus and methods are provided that enable the interaction of low energy electrons and positrons with sample ions to facilitate electron capture dissociation (EGO) and positron capture dissociation (PGO), respectively, within multipole ion guide structures.

摘要翻译： 提供了能够使低能电子和正电子与样品离子相互作用的装置和方法，以便分别在多极离子导向结构内的电子俘获解离（EGO）和正电子俘获解离（PGO）。

公开(公告)号：US08686356B2

公开(公告)日：2014-04-01

申请号：US13714089

申请日：2012-12-13

申请人： PerkinElmer Health Sciences, Inc.

发明人： Craig M. Whitehouse ,  David G. Welkie ,  Gholamreza Javahery ,  Lisa Cousins ,  Sergey Rakov

IPC分类号： H01J49/42 ,  H01J49/26 ,  B01D59/44

CPC分类号： H01J49/147 ,  H01J49/0054

摘要： Apparatus and methods are provided that enable the interaction of low energy electrons and positrons with sample ions to facilitate electron capture dissociation (EGO) and positron capture dissociation (PGO), respectively, within multipole ion guide structures. The apparatus and methods described herein allow EGO (and PCO) to be performed within multipole ion guides, either alone, or in combination with conventional ion fragmentation methods.

摘要翻译： 提供了能够使低能电子和正电子与样品离子相互作用的装置和方法，以便分别在多极离子导向结构内的电子俘获解离（ECD）和正电子俘获解离（PCD）。 本文所述的装置和方法允许ECD（和PCD）在多极离子导向器中单独进行，或与常规的离子碎片化方法组合进行。

公开(公告)号：US20130221233A1

公开(公告)日：2013-08-29

申请号：US13714089

申请日：2012-12-13

申请人： PerkinElmer Health Sciences, Inc.

发明人： Craig M. Whitehouse ,  David G. Welkie ,  Gholamreza Javahery ,  Lisa Cousins ,  Sergey Rakov

IPC分类号： H01J49/14

CPC分类号： H01J49/147 ,  H01J49/0054

摘要： Apparatus and methods are provided that enable the interaction of low-energy electrons and positrons with sample ions to facilitate electron capture dissociation (ECD) and positron capture dissociation (PCD), respectively, within multipole ion guide structures. It has recently been discovered that fragmentation of protonated ions of many biomolecules via ECD often proceeds along fragmentation pathways not accessed by other dissociation methods, leading to molecular structure information not otherwise easily obtainable. However, such analyses have been limited to expensive Fourier transform ion cyclotron resonance (FTICR) mass spectrometers; the implementation of ECD within commonly-used multipole ion guide structures is problematic due to the disturbing effects of RF fields within such devices. The apparatus and methods described herein successfully overcome such difficulties, and allow ECD (and PCD) to be performed within multipole ion guides, either alone, or in combination with conventional ion fragmentation methods. Therefore, improved analytical performance and functionality of mass spectrometers that utilize multipole ion guides are provided.

摘要翻译： 提供了能够使低能电子和正电子与样品离子相互作用的装置和方法，以便分别在多极离子导向结构内的电子俘获解离（ECD）和正电子俘获解离（PCD）。 最近已经发现，许多生物分子通过ECD的质子化离子的碎裂通常沿着不被其它解离方法接近的断裂途径进行，导致分子结构信息不容易获得。 然而，这种分析仅限于昂贵的傅里叶变换离子回旋共振（FTICR）质谱仪; 在常用的多极离子导向结构中的ECD的实现是由于RF场在这种装置内的干扰效应而成问题的。 本文描述的装置和方法成功地克服了这些困难，并且允许ECD（和PCD）在多极离子导向器中单独进行，或者与传统的离子碎裂方法组合进行。 因此，提供了利用多极离子导向器的质谱仪的改进的分析性能和功能性。