-
1.发明申请公开(公告)号：US20210292697A1
公开(公告)日：2021-09-23
申请号：US17330503
申请日：2021-05-26
Inventor： KIYOTAKA TSUJI
Abstract: A cellular potential measurement substrate includes an insulating substrate having, on a main surface thereof, a culture region for culturing a cell, a measurement electrode disposed in the culture region and configured to measure a potential of the cell, a plurality of insulating fibers arranged on the culture region side by side at intervals in a Y-direction and each extending in an X-direction, a frame plate disposed on the plurality of insulating fibers and having a culture hole at a position corresponding to the culture region, and an adhesive that has bonded the frame plate to the insulating substrate. The frame plate further has a first adhesion through-hole into which the adhesive has been injected. The first adhesion through-hole is disposed in a first region that does not overlap the culture hole when viewed in the first direction.
-
公开(公告)号：US20210348097A1
公开(公告)日：2021-11-11
申请号：US17231070
申请日：2021-04-15
Inventor： TAICHI NAKAMURA , NORIHITO TSUKAHARA , KOUJI IKEDA , KIYOTAKA TSUJI
Abstract: A cell culture chip has a stack structure formed by sequentially stacking: a first electrode provided on a main surface of a first board; a first partition wall layer including a first main flow path, and a first inlet flow path and a first outlet flow path connected to the first main flow path; a planar mesh structure sheet used as a scaffolding material for cells; a second partition wall layer including a second main flow path, and a second inlet flow path and a second outlet flow path connected to the second main flow path; and a second electrode provided on a main surface of a second board, in which the planar mesh structure sheet is sandwiched between the first partition wall layer and the second partition wall layer, and, among aperture ratios of a surface of the planar mesh structure sheet facing the first partition wall layer, an aperture ratio of a portion facing the first main flow path is greater than an aperture ratios of portions facing the first inlet flow path and the first outlet flow path, and, among aperture ratios of a surface of the planar mesh structure sheet facing the second partition wall layer, an aperture ratio of a portion facing the second main flow path is greater than an aperture ratios of portions facing the second inlet flow path and the second outlet flow path.
-
3.发明申请公开(公告)号：US20200333285A1
公开(公告)日：2020-10-22
申请号：US16920356
申请日：2020-07-02
Inventor： YUMIKO KATO , KIYOTAKA TSUJI
IPC: G01N27/416 , G01N27/30 , C12M1/34
Abstract: An electric reaction measuring apparatus measures a potential of each of measurement electrodes, which are respectively disposed in chambers in a culture vessel, with respect to at least one reference electrode. The electric reaction measuring apparatus includes at least one control circuit. A measurement object is disposed in each of the chambers. The at least one control circuit calculates at least one principal component for each of the measurement electrodes by performing principal component analysis of the potential of each of the measurement electrodes; estimates, for each of the measurement electrodes, a potential corresponding to the principal component from the principal component of the measurement electrode, and synthesizes the estimated potential; subtracts the synthesized potential of the measurement electrode from a potential measured at the measurement electrode; and outputs a potential after subtraction.
-
公开(公告)号：US20240264111A1
公开(公告)日：2024-08-08
申请号：US18635022
申请日：2024-04-15
Inventor： KIYOTAKA TSUJI
IPC: G01N27/327 , G01N27/333
CPC classification number: G01N27/3273 , G01N27/3275 , G01N27/3335
Abstract: The present disclosure provides an electrochemical reaction device that is advantageous from the viewpoint of energy efficiency while continuously oxidizing NAD and decomposing glucose. An electrochemical reaction device of the present disclosure includes a first tank, a second tank, a membrane, and a voltage application device. The first tank accommodates glucose dehydrogenase and nicotinamide adenine dinucleotide. Further, a working electrode is disposed in the first tank. A counter electrode is disposed in the second tank. The voltage application device applies a voltage between the working electrode and the counter electrode. The membrane separates the inside of the first tank and the inside of the second tank from each other. The membrane blocks permeation of the glucose dehydrogenase and the nicotinamide adenine dinucleotide and has ion conductivity.
-
公开(公告)号：US20240263212A1
公开(公告)日：2024-08-08
申请号：US18635081
申请日：2024-04-15
Inventor： KIYOTAKA TSUJI
IPC: C12Q1/00 , G01N27/327
CPC classification number: C12Q1/005 , G01N27/3271 , G01N27/3275
Abstract: The present disclosure provides a control method for a treatment device which is advantageous from the viewpoint of coping with change of a component contained in a treatment target while a reaction system including an oxidoreductase and a coenzyme is used. An enzyme electrode used in the control method for the treatment device according to the present disclosure includes a first conductor, a second conductor, and an enzyme portion. The second conductor is electrically insulated from the first conductor. The enzyme portion is fixed to a surface of the first conductor. The enzyme portion includes the oxidoreductase and the coenzyme. The second conductor has a surface to which only one enzyme selected from the group consisting of the oxidoreductase and the coenzyme is fixed, or to which a portion including both the oxidoreductase and the coenzyme is not fixed.
-
Patent Agency Ranking
6.发明公开公开(公告)号：US20240252072A1
公开(公告)日：2024-08-01
申请号：US18633575
申请日：2024-04-12
Inventor： KIYOTAKA TSUJI
IPC: A61B5/1486 , A61B5/145 , A61B5/157
CPC classification number: A61B5/14865 , A61B5/157 , A61B5/14532
Abstract: The present disclosure provides a method for controlling a treatment apparatus having advantages from the viewpoint of dealing with a change in a component contained in a treatment target while using a reaction system containing a redox enzyme and a coenzyme. The method for controlling a treatment apparatus according to the present disclosure includes acquiring of a first relationship R1 between a first voltage value and a first current value corresponding to the first voltage value by applying a voltage between a first electrode and a second electrode in a state where a treatment liquid does not contain the redox enzyme or the coenzyme. The controlling method includes acquiring of a second relationship R2 between a second voltage value and a second current value corresponding to the second voltage value by applying a voltage between the first electrode and the second electrode in a state where the treatment liquid contains the redox enzyme and the coenzyme. The controlling method determines a voltage Vt for treating the treatment liquid and a target current Ia serving as an indicator of completion of treatment of the treatment liquid in accordance with the first relationship R1 and the second relationship R2.
-
公开(公告)号：US20220195374A1
公开(公告)日：2022-06-23
申请号：US17690054
申请日：2022-03-09
Inventor： KIYOTAKA TSUJI
Abstract: A cell culture chip comprises a body having a first flow channel and a second flow channel at least part of which overlaps the first flow channel when seen from an angle parallel with a predetermined direction, a cell separation membrane having first and second principal surfaces facing away from each other, the cell separation membrane being disposed between the first flow channel and the second flow channel so that the first flow channel is located on the first principal surface and the second flow channel is located on the second principal surface, a first electrode which is in contact with the first flow channel and extending through the first flow channel along the first flow channel, and a second electrode which is in contact with the second flow channel and extending through the second flow channel along the second flow channel.
-
公开(公告)号：US20220073864A1
公开(公告)日：2022-03-10
申请号：US17392423
申请日：2021-08-03
Inventor： NORIHITO TSUKAHARA , TAICHI NAKAMURA , KOUJI IKEDA , KIYOTAKA TSUJI
Abstract: A fiber sheet of the present disclosure includes: a first fiber layer including a plurality of first fibers, the plurality of first fibers comprising a thermoplastic polymer and arranged side by side in a first direction; a second fiber layer including a plurality of second fibers, the plurality of second fibers comprising a thermoplastic polymer and arranged side by side in a second direction intersecting the first direction, and disposed to face the first fiber layer; and a nanofiber layer including nanofibers, the nanofibers comprising any one of a thermoplastic polymer, a thermosetting polymer, a biodegradable polymer, and a biological polymer, the nanofiber layer disposed to be in contact with the first fiber layer and the second fiber layer, in which the nanofiber layer is heat-welded to the first fiber layer and the second fiber layer.
-
公开(公告)号：US20210285138A1
公开(公告)日：2021-09-16
申请号：US17198185
申请日：2021-03-10
Inventor： TAICHI NAKAMURA , NORIHITO TSUKAHARA , KOUJI IKEDA , KIYOTAKA TSUJI
Abstract: A fiber mesh sheet is provided, in which the fiber mesh sheet has a mesh structure in which two or more layers of planar fiber arrangement groups are laminated, where, in each of the planar fiber arrangement groups, longitudinal directions of a plurality of fibers made of a polymer material are arranged in a plane along one direction, longitudinal directions of the fibers in one of the fiber arrangement groups intersect those of the fibers in the other fiber arrangement group in two adjacent layers of the fiber arrangement groups at an intersecting angle of 30° or more and 150° or less in a plan view seen from a direction perpendicular to the plane, an upper part of a cross section of the fiber in the fiber arrangement group at a lowermost layer is a substantially circular shape, and a lower part of a cross section of the fiber in the fiber arrangement group at the lowermost layer is a substantially flat shape, where the upper part is a side on which the adjacent fiber arrangement group is present, and the lower part is a side on which the adjacent fiber arrangement group is not present, and a cross section of the fiber in the fiber arrangement group at a layer other than the lowermost layer is a substantially circular shape.
-
公开(公告)号：US20200232967A1
公开(公告)日：2020-07-23
申请号：US16842902
申请日：2020-04-08
Inventor： YUMIKO KATO , YOSHIHIDE SAWADA , TAICHI SATO , KIYOTAKA TSUJI
IPC: G01N33/483 , G06T7/00 , G06T7/11 , G06T7/557 , G06T7/62
Abstract: The present disclosure provides a technique which makes it possible to evaluate a state of a cell aggregation of one or more spheroids. In the culture state determination device according to the present disclosure, a plurality of light sources sequentially illuminate a plurality of cell aggregations put on an image sensor. The image sensor acquires captured images of the plurality of the cell aggregations each time when the plurality of the light sources illuminate the plurality of the cell aggregations. Control circuitry extracts a region including an image of the cell aggregation in the captured image; generates three-dimensional image information of the region using a plurality of the captured images; extracts an outer shape of the cell aggregation and a cavity part inside the cell aggregation using the three-dimensional image information; calculates a first volume that is a volume based on the outer shape of each of the cell aggregation and a second volume that is a volume of the cavity part based on the cavity part of each of the cell aggregation in the three-dimensional image information; and determines a culture state of the cell aggregations using the first volume and the second volume.
-
-
-
-
-
-
-
-
-
Search Results
10
records
(took 0.019 seconds)
