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公开(公告)号:US20230279413A1
公开(公告)日:2023-09-07
申请号:US18056002
申请日:2022-11-16
发明人: ANDREW MARK CIGAN , SAVERIO CARL FALCO , HUIRONG GAO , ZHONGSEN LI , ZHAN-BIN LIU , L. ALEKSANDER LYZNIK , JINRUI SHI , SERGEI SVITASHEV , JOSHUA K. YOUNG
CPC分类号: C12N15/8216 , C12N15/00 , C12N15/63 , C12N15/8213 , C12N15/8247 , C12N15/8274 , A01H1/00
摘要: Compositions and methods are provided for genome modification of a target sequence in the genome of a plant or plant cell. The methods and compositions employ a guide RNA/Cas endonuclease system to provide an effective system for modifying or altering target sites within the genome of a plant, plant cell or seed. Also provided are compositions and methods employing a guide polynucleotide/Cas endonuclease system for genome modification of a nucleotide sequence in the genome of a cell or organism, for gene editing, and/or for inserting or deleting a polynucleotide of interest into or from the genome of a cell or organism. Once a genomic target site is identified, a variety of methods can be employed to further modify the target sites such that they contain a variety of polynucleotides of interest. Breeding methods and methods for selecting plants utilizing a two component RNA guide and Cas endonuclease system are also disclosed. Compositions and methods are also provided for editing a nucleotide sequence in the genome of a cell.
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2.
公开(公告)号:US20210238614A1
公开(公告)日:2021-08-05
申请号:US17053663
申请日:2019-05-07
IPC分类号: C12N15/82
摘要: Methods and compositions are provided for the improvement of homology-directed repair of a double strand break in a plant cell, via the use of a polynucleotide comprising sequences homologous to the target site. In some aspects, the double strand break is created by an RNA-guided Cas endonuclease. The homology-directed repair of the double-strand break may include incorporation of a heterologous polynucleotide, for example a gene encoding a trait of agronomic importance. The homology-directed repair of the double-strand break may occur as a result of template-directed repair using a polynucleotide repair template.
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公开(公告)号:US20210062201A1
公开(公告)日:2021-03-04
申请号:US16925973
申请日:2020-07-10
发明人: ANDREW MARK CIGAN , SAVERIO CARL FALCO , HUIRONG GAO , MICHAEL LASSNER , DEREK JANTZ , ZHONGSEN LI , ZHAN-BIN LIU , SERGEI SVITASHEV , JAMES JEFFERSON SMITH
摘要: Methods for producing in a plant a complex transgenic trait locus comprising at least two altered target sequences in a genomic region of interest are disclosed. The methods involve the use of two or more double-strand-break-inducing agents, each of which can cause a double-strand break in a target sequence in the genomic region of interest which results in an alteration in the target sequence. Also disclosed are complex transgenic trait loci in plants. A complex transgenic trait locus comprises at least two altered target sequences that are genetically linked to a polynucleotide of interest. Plants, plant cells, plant parts, and seeds comprising one or more complex transgenic trait loci are also disclosed.
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公开(公告)号:US20230380373A1
公开(公告)日:2023-11-30
申请号:US18250912
申请日:2021-10-22
发明人: HYEON-JE CHO , JOHN D. EVERARD , ANTHONY KINNEY , ZHAN-BIN LIU , KNUT MEYER , THOMAS G. PATTERSON , KEVIN G. RIPP , BO SHEN
CPC分类号: A01H6/542 , A01H5/10 , A01H1/101 , C12N15/8251
摘要: Soybean plants producing soybean seeds comprising leghemoglobin are produced by modifying the genome of the soybean plant. Soybean plants, soybean seeds and soy protein compositions comprising leghemoglobin are provided. Soybean plants, soybean seeds and soy protein compositions comprising leghemoglobin and additionally one or more of high oleic acid, low linolenic acid, high protein, low stachyose, low raffinose and low protease inhibitors are provided. Protein compositions comprising leghemoglobin, such as soy isolates and concentrates can be made from the soybean seeds. Additionally, methods for generating and using plants, seeds and protein compositions comprising leghemoglobin are disclosed.
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公开(公告)号:US20230235342A1
公开(公告)日:2023-07-27
申请号:US17821528
申请日:2022-08-23
发明人: ANDREW MARK CIGAN , SAVERIO CARL FALCO , HUIRONG GAO , MICHAEL LASSNER , DEREK JANTZ , ZHONGSEN LI , ZHAN-BIN LIU , SERGEI SVITASHEV , JAMES JEFFERSON SMITH
CPC分类号: C12N15/8202 , A01H1/06 , C12N9/22 , C12N15/8213
摘要: Methods for producing in a plant a complex transgenic trait locus comprising at least two altered target sequences in a genomic region of interest are disclosed. The methods involve the use of two or more double-strand-break-inducing agents, each of which can cause a double-strand break in a target sequence in the genomic region of interest which results in an alteration in the target sequence. Also disclosed are complex transgenic trait loci in plants. A complex transgenic trait locus comprises at least two altered target sequences that are genetically linked to a polynucleotide of interest. Plants, plant cells, plant parts, and seeds comprising one or more complex transgenic trait loci are also disclosed.
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公开(公告)号:US20230151338A1
公开(公告)日:2023-05-18
申请号:US17933358
申请日:2022-09-19
发明人: KAYLA S FLYCKT , KRISTIN HAUG COLLET , ZHAN-BIN LIU , KEITH R ROESLER , BO SHEN , LAURA L WAYNE
CPC分类号: C12N9/1029 , C12N15/8213 , C12N15/8247 , C12Y203/0102
摘要: Provided are compositions comprising polynucleotides encoding modified diacylglycerol acyltransferase-1 (DGAT1) polypeptides having improved properties, such as increased enzymatic activity and/or increased stability. Plants, plant cells, seed, grain and comprising the polynucleotides are provided which have one or more of increased fatty acid or protein content. Methods of generating the polynucleotides in plant cells include transformation and genetic modification. Methods of employing the polynucleotides in plants, methods for increasing DGAT1 activity in a plant, and methods for increasing fatty acid content or protein content in a plant are provided.
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公开(公告)号:US20220127631A1
公开(公告)日:2022-04-28
申请号:US17451913
申请日:2021-10-22
发明人: HYEON-JE CHO , JOHN D EVERARD , ANTHONY J KINNEY , ZHAN-BIN LIU , KNUT MEYER , THOMAS G PATTERSON , KEVIN G RIPP , BO SHEN
IPC分类号: C12N15/82 , C07K14/415
摘要: Soybean plants producing soybean seeds comprising leghemoglobin are produced by modifying the genome of the soybean plant. Soybean plants, soybean seeds and soy protein compositions comprising leghemoglobin are provided. Soybean plants, soybean seeds and soy protein compositions comprising leghemoglobin and additionally one or more of high oleic acid, low linolenic acid, high protein, low stachyose, low raffinose and low protease inhibitors are provided. Protein compositions comprising leghemoglobin, such as soy isolates and concentrates can be made from the soybean seeds. Additionally, methods for generating and using plants, seeds and protein compositions comprising leghemoglobin are disclosed.
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公开(公告)号:US20210403933A1
公开(公告)日:2021-12-30
申请号:US17293532
申请日:2019-11-12
发明人: JOHN RUSSELL BOOTH, Jr. , ZHAN-BIN LIU , CHENG LU , BO SHEN
IPC分类号: C12N15/82
摘要: Soybean seeds with increased protein and having a modified expression or activity of at least one or two HECT E3 ligase polypeptides are provided. Methods for modifying expression or activity of HECT E3 ligase polypeptides and polynucleotides include genome editing to modify the transcription regulatory region or sequence encoding the HECT E3 ligase polypeptides and transformation with recombinant DNA constructs to enhance or suppress expression or activity of the HECT E3 ligase polypeptides. Plants containing the modifications produce seeds with altered composition such as one or more of increased protein, decreased soluble carbohydrate, increased oleic acid, decreased saturated fats such as palmitic and stearic acids, and decreased linoleic or linolenic acid.
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公开(公告)号:US20210207103A1
公开(公告)日:2021-07-08
申请号:US17057056
申请日:2019-05-30
发明人: KAYLA S FLYCKT , KRISTIN HAUG COLLET , ZHAN-BIN LIU , KEITH R ROESLER , BO SHEN , LAURA L WAYNE
摘要: Provided are compositions comprising polynucleotides encoding modified diacylglycerol acyltransferase-1 (DGAT1) polypeptides having improved properties, such as increased enzymatic activity and/or increased stability. Plants, plant cells, seed, grain and comprising the polynucleotides are provided which have one or more of increased fatty acid or protein content. Methods of generating the polynucleotides in plant cells include transformation and genetic modification. Methods of employing the polynucleotides in plants, methods for increasing DGAT1 activity in a plant, and methods for increasing fatty acid content or protein content in a plant are provided.
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10.
公开(公告)号:US20230323374A1
公开(公告)日:2023-10-12
申请号:US18055614
申请日:2022-11-15
发明人: ANDREW MARK CIGAN , SAVERIO CARL FALCO , HUIRONG GAO , ZHONGSEN LI , ZHAN-BIN LIU , L. ALEKSANDER LYZNIK , JINRUI SHI , SERGEI SVITASHEV , JOSHUA K. YOUNG
CPC分类号: C12N15/8216 , C12N15/00 , C12N15/63 , C12N15/8213 , C12N15/8247 , C12N15/8274 , A01H1/00
摘要: Compositions and methods are provided for genome modification of a target sequence in the genome of a plant or plant cell. The methods and compositions employ a guide RNA/Cas endonuclease system to provide an effective system for modifying or altering target sites within the genome of a plant, plant cell or seed. Also provided are compositions and methods employing a guide polynucleotide/Cas endonuclease system for genome modification of a nucleotide sequence in the genome of a cell or organism, for gene editing, and/or for inserting or deleting a polynucleotide of interest into or from the genome of a cell or organism. Once a genomic target site is identified, a variety of methods can be employed to further modify the target sites such that they contain a variety of polynucleotides of interest. Breeding methods and methods for selecting plants utilizing a two component RNA guide and Cas endonuclease system are also disclosed. Compositions and methods are also provided for editing a nucleotide sequence in the genome of a cell.
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