公开(公告)号：US20210238614A1

公开(公告)日：2021-08-05

申请号：US17053663

申请日：2019-05-07

Applicant: PIONEER HI-BRED INTERNATIONAL, INC.

Inventor： AJITH ANAND ,  WILLIAM JAMES GORDON-KAMM ,  SANDEEP KUMAR ,  ZHAN-BIN LIU ,  SERGI SVITASHEV

IPC: C12N15/82

Abstract: Methods and compositions are provided for the improvement of homology-directed repair of a double strand break in a plant cell, via the use of a polynucleotide comprising sequences homologous to the target site. In some aspects, the double strand break is created by an RNA-guided Cas endonuclease. The homology-directed repair of the double-strand break may include incorporation of a heterologous polynucleotide, for example a gene encoding a trait of agronomic importance. The homology-directed repair of the double-strand break may occur as a result of template-directed repair using a polynucleotide repair template.

公开(公告)号：US20210087573A1

公开(公告)日：2021-03-25

申请号：US17052320

申请日：2019-05-07

Applicant: PIONEER HI-BRED INTERNATIONAL, INC.

Inventor： STEPHEN LAWRENCE GASIOR ,  SERGI SVITASHEV ,  JOSHUA K YOUNG

IPC: C12N15/82 ,  C12N9/22 ,  C12N15/90 ,  C12N15/11

Abstract: Compositions and methods are provided for effecting homology-directed repair at the site of a double-strand-break repair in a polynucleotide. In some aspects, the frequency of HDR is improved as compared to a control method. In some aspects, the ratio of HR to NHEJ is increased. In some aspects, the percentage of HR relative to the total number of mutant reads is increased.