公开(公告)号：US20140186863A1

公开(公告)日：2014-07-03

申请号：US14199136

申请日：2014-03-06

Applicant: OLYMPUS CORPORATION

Inventor： Hiromi SANUKI ,  Nao MORIYA ,  Rie KATAOKA

IPC: G01N33/574

CPC classification number: G01N33/57438

Abstract: The present invention relates to a method for detecting a marker for pancreatic disease for obtaining highly reliable results while reducing the risk of complications. It also relates to a method for a detecting a marker for pancreatic disease comprising: measuring the concentration of a marker for pancreatic disease in duodenal juice that is collected from a subject and contains spontaneously discharged pancreatic juice, by a measurement method including detecting antigen or antibody immobilized on a solid phase using an antigen-antibody reaction, and the aforementioned method for detecting a marker for pancreatic disease further including judging the subject to have the possibility of being afflicted with a pancreatic disease in the case the concentration of the marker for pancreatic disease is equal to or greater than a prescribed threshold value.

Abstract translation: 本发明涉及一种用于检测胰腺疾病标志物的方法，以获得高度可靠的结果，同时降低并发症的风险。 本发明还涉及一种胰腺疾病标志物的检测方法，其特征在于，包括：检测受试者收集的十二指肠液中胰腺疾病的标记物浓度，并含有自发排出的胰液，包括检测抗原或抗体 使用抗原 - 抗体反应固定在固相上，以及上述用于检测胰腺疾病标志物的方法，其进一步包括判断受试者在胰腺疾病标志物浓度的情况下可能患有胰腺疾病 等于或大于规定的阈值。

公开(公告)号：US20140030823A1

公开(公告)日：2014-01-30

申请号：US14043267

申请日：2013-10-01

Applicant: OLYMPUS CORPORATION

Inventor： Hiromi SANUKI ,  Rie KATAOKA ,  Nao MORIYA

IPC: G01N21/78

CPC classification number: G01N21/78 ,  A61B5/4216 ,  A61B10/0045 ,  C12Q1/68 ,  G01N33/487 ,  G01N33/57438 ,  G01N33/6893 ,  G01N2800/067

Abstract: A pancreatic disease is tested for with high sensitivity even with simple equipment and a simple procedure. Provided is a method of detecting pancreatic disease including detecting a concentration of S100P in at least one of a pancreatic juice and a body fluid containing pancreatic juice collected from a test subject by immunochromatography. Additionally provided is a pancreas testing kit including an immunochromatography device that holds an anti-S100P antibody and a collection vessel that retains a protease inhibitor that inhibits an activity of a protease contained in the pancreatic juice.

Abstract translation: 即使使用简单的设备和简单的程序，胰腺疾病也会以高灵敏度进行测试。 本发明提供一种检测胰腺疾病的方法，包括通过免疫色谱法检测从受试者收集的含有胰液的胰液和体液中的至少一种中S100P的浓度。 另外提供了胰腺检测试剂盒，其包括含有抗S100P抗体的免疫层析装置和保留抑制胰液中所含蛋白酶活性的蛋白酶抑制剂的收集容器。

公开(公告)号：US20140186869A1

公开(公告)日：2014-07-03

申请号：US14200188

申请日：2014-03-07

Applicant: OLYMPUS CORPORATION

Inventor： Rie KATAOKA ,  Mari NAKATA ,  Nao MORIYA

IPC: C12Q1/37

CPC classification number: C12Q1/37 ,  A01N1/00 ,  C07K14/81

Abstract: This method for inhibiting protease in a biological sample containing pancreatic juice components inhibits protease enzyme activity in the biological sample by adding at least one type of protease inhibitor having a sulfonyl fluoride group to the biological sample containing pancreatic juice components. In addition, this protease inhibitor for a biological sample containing pancreatic juice components is a compound having a sulfonyl fluoride group, has protease inhibitory activity, and is added to a biological sample containing pancreatic juice components in order to inhibit protease present in the biological sample. Moreover, this kit for preserving a biological sample containing pancreatic juice components contains at least one type of protease inhibitory having a sulfonyl fluoride group, and is used to store a biological sample containing pancreatic juice components.

Abstract translation: 用于抑制含有胰液成分的生物样品中的蛋白酶的方法通过向含有胰液成分的生物样品中添加至少一种具有磺酰氟基团的蛋白酶抑制剂来抑制生物样品中的蛋白酶活性。 此外，这种含有胰液成分的生物样品的蛋白酶抑制剂是具有磺酰氟基团的化合物，具有蛋白酶抑制活性，并且添加到含有胰液成分的生物样品中以抑制存在于生物样品中的蛋白酶。 此外，用于保存含有胰汁成分的生物样品的该试剂盒含有至少一种具有磺酰氟基团的蛋白酶抑制剂，并用于储存含有胰汁成分的生物样品。

公开(公告)号：US20150104846A1

公开(公告)日：2015-04-16

申请号：US14576437

申请日：2014-12-19

Applicant: OLYMPUS CORPORATION

Inventor： Rie KATAOKA ,  Nao MORIYA ,  Hiromi SANUKI

IPC: C12N9/99 ,  C09K15/28

CPC classification number: C12N9/99 ,  C09K15/28 ,  G01N33/57438

Abstract: The present invention provides a method for preserving biological components in pancreatic juice, and a kit suited for this method. The method involves adding, to a pancreatic juice-containing biological sample, at least one sulfonyl fluoride-containing protease inhibitor and at least one trypsin-like serine protease inhibitor belonging to a group of amino acid chloromethyl ketones.

Abstract translation: 本发明提供了一种保存胰汁中生物成分的方法，以及适用于该方法的试剂盒。 该方法包括向含有胰液的生物样品中加入至少一种含磺酰氟的蛋白酶抑制剂和至少一种属于一组氨基酸氯甲基酮的胰蛋白酶样丝氨酸蛋白酶抑制剂。