公开(公告)号：US20130250391A1

公开(公告)日：2013-09-26

申请号：US13801899

申请日：2013-03-13

Applicant: OLYMPUS CORPORATION

Inventor： Jun KATO ,  Hiroshi SASAKI ,  Yujin ARAI

IPC: G02B21/06 ,  G02B21/36

CPC classification number: G02B21/06 ,  G02B21/082 ,  G02B21/16 ,  G02B21/361 ,  G02B21/365 ,  G02B21/367

Abstract: A laser microscope includes an objective lens that radiates a laser beam onto a specimen; a stimulation optical system having an LCOS-SLM located at a position optically conjugate with the pupil position of the objective lens and which modulates the phase of the laser beam; and an observation optical system having a galvanometer mirror that scans the laser beam across the specimen, as observation illuminating light, and a PMT that detects the observation light coming from the specimen and collected by the objective lens. A control unit forms a three-dimensional image of the specimen and sets, in that image, stimulation sites in the specimen to be irradiated with a laser beam serving as a stimulation beam by the stimulation optical system at a plurality of different positions in the optical axis direction. The LCOS-SLM modulates the laser beam such that the stimulation sites are irradiated with the laser beam.

Abstract translation: 激光显微镜包括将激光束照射到样品上的物镜; 刺激光学系统，其具有位于与物镜的光瞳位置光学共轭的位置并且调制激光束的相位的LCOS-SLM; 以及具有扫描横跨试样的激光束作为观察照明光的检流计镜的观察光学系统，以及检测来自样本并由物镜收集的观察光的PMT。 控制单元形成样本的三维图像，并且在该图像中，通过刺激光学系统在光学器件中的多个不同位置处设置用于作为刺激光束的激光束照射的样本中的刺激部位 轴方向。 LCOS-SLM调制激光束，使激光位置用激光束照射。