公开(公告)号：US20220252580A1

公开(公告)日：2022-08-11

申请号：US17603598

申请日：2019-04-23

申请人： Miltenyi Biotec B.V. & Co. KG

发明人： Christian DOSE ,  Jennifer PANKRATZ

IPC分类号： G01N33/533 ,  G01N1/30

摘要： The invention is directed to a conjugate for labelling a target moiety on a cell, characterized with the general formula (I) (Xo-L)n-P-Ym, with Y: antigen recognizing moiety recognizing the target moiety, P: enzymatically degradable spacer, X: fluorescent moiety, L: linker unit comprising one or more polyethyleneglycol residues n, m: integer between 1 and 100, o integer between 1 and 100 wherein L covalent bounds the fluorescent moiety X and the enzymatically degradable spacer P and Y is covalently bound to the enzymatically degradable spacer P and wherein the enzymatically degradable spacer P is selected from the group consisting of polysaccharides, polyesters, nucleic acids, and derivatives thereof. Method of detecting a target moiety in a sample of biological specimen with the conjugate.

公开(公告)号：US20230013963A1

公开(公告)日：2023-01-19

申请号：US17782172

申请日：2020-12-09

申请人： Miltenyi Biotec B.V. & Co. KG

发明人： Jennifer PANKRATZ ,  Jennifer BRIEDEN ,  Cesar EVARISTO ,  Volker NOLLE ,  Marek WIECZOREK

IPC分类号： G01N33/50 ,  G01N33/533

摘要： The invention is directed to a Conjugate for labelling a target moiety on a cell, characterized by general formula (II): Xo-P-Ym, with Y: MHC-complex targeting TCR molecules, P: enzymatically degradable spacer, X: detection moiety, o: integer between 5 and 25, m: integer between 2 and 1000, wherein X and P; P and Y are covalently bound to each other and Y is bound to P via the C-terminus.

公开(公告)号：US20210018498A1

公开(公告)日：2021-01-21

申请号：US17035833

申请日：2020-09-29

申请人： Miltenyi Biotec B.V. & Co. KG

发明人： Jennifer PANKRATZ ,  Christian DOSE ,  Mario ASSENMACHER

IPC分类号： G01N33/542 ,  G01N33/53 ,  G01N33/537

摘要： The invention is directed to a Method for detecting a target moiety in a sample of biological specimens by: a) providing at least one conjugate with the general formula (I) An-P-Bm-Cq-Xo  (I) with A: antigen recognizing moiety; P: enzymatically degradable spacer; B: first binding moiety C second binding moiety X: detection moiety; n, m, q, o integers between 1 and 100, wherein B and C are non-covalently bound to each other and A and B are covalently bound to P b) labelling the target moiety recognized by the antigen recognizing moiety A with at least one conjugate c) detecting the labelled target moiety via detecting moiety X d) cleaving Cq-Xo by disrupting the non-covalent bond between Bm and Cq from the labelled target moiety e) cleaving the binding moiety Bm from the labelled target moiety by enzymatically degrading spacer P. The method is useful to identify target moieties on the biological specimens. The biological specimens detected by the conjugate can be subsequently removed from the sample.