公开(公告)号：US20230333090A1

公开(公告)日：2023-10-19

申请号：US17786039

申请日：2020-12-16

申请人： Merck Sharp & Dohme LLC

发明人： Kevin B. Gurney ,  Yue Zhang ,  Linda Liang ,  Cuiwei Ai

IPC分类号： G01N33/50 ,  C12N5/0783

CPC分类号： G01N33/505 ,  C12N5/0636 ,  G01N33/5023 ,  G01N2333/55 ,  G01N2333/70503

摘要： The present disclosure describes a murine T cell 3A9 comprising a polynucleotide sequence encoding human LAG3, and a murine IL-2 promotor operably associated with a reporter gene. The disclosure also describes a cell-based potency assay for measuring LAG3 antagonist activity using a co-cultured system with the murine T cells and LK35.2 murine B cells, and hen egg lysozyme peptide.