公开(公告)号：US5011676A

公开(公告)日：1991-04-30

申请号：US499790

申请日：1990-03-27

Applicant: Mathew L. Thakur

Inventor： Mathew L. Thakur

IPC: A61K51/10 ,  A61K51/12

CPC classification number: A61K51/1045 ,  A61K51/10 ,  A61K51/1018 ,  A61K51/121 ,  A61K2123/00 ,  Y10S530/864

Abstract: The invention is a novel method and kit for directly radiolabeling proteins such as antibodies or antibody fragments for diagnostic and therapeutic purposes. The method comprises incubating a protein-containing solution with a solution of sodium ascorbate; adding a required quantity of reduced radionuclide to the incubated protein. A kit is also provided wherein the protein and/or reducing agents may be in lyophilized form.

Abstract translation: 本发明是用于诊断和治疗目的的用于直接放射性标记诸如抗体或抗体片段的蛋白质的新型方法和试剂盒。 该方法包括将含蛋白质的溶液与抗坏血酸钠溶液一起孵育; 向培养的蛋白质中加入所需量的还原放射性核素。 还提供了一种试剂盒，其中蛋白质和/或还原剂可以是冻干形式。

公开(公告)号：US5308603A

公开(公告)日：1994-05-03

申请号：US591964

申请日：1990-10-02

Applicant: Mathew L. Thakur

Inventor： Mathew L. Thakur

IPC: A61K51/10 ,  A61K51/12 ,  A61K49/02 ,  A61K43/00

CPC classification number: A61K51/1045 ,  A61K51/10 ,  A61K51/1018 ,  A61K51/121 ,  A61K2123/00 ,  Y10S530/864

Abstract: The invention is a novel method and kit for directly radiolabeling proteins such as antibodies or antibody fragments for diagnostic and therapeutic purposes. The method comprises incubating a protein-containing solution with a solution of sodium ascorbate; adding a required quantity of reduced radionuclide to the incubated protein. A kit is also provided wherein the protein and/or reducing agents may be in lyophilized form.

Abstract translation: 本发明是用于诊断和治疗目的的用于直接放射性标记诸如抗体或抗体片段的蛋白质的新型方法和试剂盒。 该方法包括将含蛋白质的溶液与抗坏血酸钠溶液一起孵育; 向培养的蛋白质中加入所需量的还原放射性核素。 还提供了一种试剂盒，其中蛋白质和/或还原剂可以是冻干形式。

公开(公告)号：US20110123988A1

公开(公告)日：2011-05-26

申请号：US12778376

申请日：2010-05-12

Applicant: Eric Wickstrom ,  Mathew L. Thakur

Inventor： Eric Wickstrom ,  Mathew L. Thakur

IPC: C12Q1/68 ,  C07K9/00

CPC classification number: C12N15/1135 ,  A61K51/088 ,  A61K51/1268 ,  B82Y5/00 ,  C12N15/111 ,  C12N15/1138 ,  C12N2310/3181 ,  C12N2310/3513 ,  C12N2310/3517 ,  C12N2320/32

Abstract: Compounds comprising a diagnostic or therapeutic moiety can be retained inside a cell by conjugating the moiety to at least one PNA that is targeted to the transcripts from a gene of interest. The diagnostic or therapeutic moiety is also conjugated to at least one targeting moiety specific for an extracellular receptor or other cell surface molecule. The targeting moiety binds to the surface of a cell, and the entire compound is then internalized. Once inside the cell, the PNA portion of the diagnostic or therapeutic compound binds to RNA transcripts in a sequence specific manner. Binding of the PNA to its target RNA transcript retains the compound within the cell. The PNA can be designed to bind to a predetermined nucleic acid sequence from an RNA transcript, for example a mutated or overexpressed sequence that is characteristic of a pathological state.

Abstract translation: 包含诊断或治疗部分的化合物可以通过将该部分与靶向目的基因的转录物的至少一种PNA结合而保留在细胞内。 诊断或治疗部分还与至少一个对细胞外受体或其他细胞表面分子特异性的靶向部分缀合。 靶向部分结合细胞的表面，然后将整个化合物内化。 一旦在细胞内，诊断或治疗化合物的PNA部分以序列特异性方式结合RNA转录物。 PNA与其靶RNA转录物的结合将该化合物保留在细胞内。 PNA可被设计成从RNA转录物例如具有病理状态特征的突变或过表达序列结合预定的核酸序列。

公开(公告)号：US5886142A

公开(公告)日：1999-03-23

申请号：US858971

申请日：1997-05-20

Applicant: Mathew L. Thakur ,  Venkataramana R. Pallela

Inventor： Mathew L. Thakur ,  Venkataramana R. Pallela

IPC: A61K51/08 ,  C07K14/78 ,  A61K38/16

CPC classification number: A61K51/088 ,  C07K14/78 ,  A61K2123/00

Abstract: This invention relates to a radiodiagnostic agent to image arterial and venous thrombi, pulmonary emboli and lesions of atherosclerosis. A composition for a thrombus imaging agent, a method and kit for preparing a thrombus imaging agent, and a radiolabeling reagent for preparing the thrombus imaging agent are provided. Methods of using the thrombus imaging agent to detect thrombi are also provided.

Abstract translation: 本发明涉及用于对动脉和静脉血栓，肺栓塞和动脉粥样硬化病变进行成像的放射诊断剂。 提供了一种用于血栓显像剂的组合物，用于制备血栓显像剂的方法和试剂盒，以及用于制备血栓显像剂的放射性标记试剂。 还提供了使用血栓成像剂检测血栓的方法。