公开(公告)号：US12209250B2

公开(公告)日：2025-01-28

申请号：US16741827

申请日：2020-01-14

Applicant: Massachusetts Institute of Technology

Inventor： Ron Weiss ,  Maria Hottelet Foley ,  Jin Huh ,  Ross D. Jones

IPC: C12N15/86 ,  A61K35/76 ,  A61K48/00 ,  C12N7/00 ,  C12N15/113

Abstract: Provided herein are engineered HSV-1 vectors comprising a modified HSV-1 genome. The engineered HSV-1 vectors can be used to deliver genetic circuits (e.g., up to 100 kb) to cells in vitro or in vivo. Methods of treating or diagnosing a disease (e.g., cancer) using the engineered HSV-1 vectors described herein are also provided.

公开(公告)号：US20230124274A1

公开(公告)日：2023-04-20

申请号：US17978490

申请日：2022-11-01

Applicant: Massachusetts Institute of Technology

Inventor： Ron Weiss ,  Giulio Alighieri

IPC: C12N15/113

Abstract: The present disclosure, at least in part, relates to a miRNA based logic gate that comprises an engineered RNA carrier that comprises an nuclear export signal, a target site for a first miRNA and a pre-miRNA sequence for a second miRNA. Also provided by the disclosure are recombinant viruses (e.g., recombinant adeno-associated viruses (rAAV)) for delivery of the miRNA based logic gates.

公开(公告)号：US20220282334A1

公开(公告)日：2022-09-08

申请号：US17688985

申请日：2022-03-08

Applicant: Massachusetts Institute of Technology

Inventor： Ron Weiss ,  Jin Huh

IPC: C12Q1/6886 ,  C12N15/113 ,  C12Q1/6811 ,  A61K31/7125 ,  C12Q1/6897

Abstract: Provided herein are genetic circuits and cell state classifiers for detecting the microRNA profile of a cell. The cell state classifiers of the present disclosure are designed to incorporate multiple genetic circuits integrated together by transcriptional or translational control. Multiple inputs can be sensed simultaneously by coupling their detection to different portions of the genetic circuit such that the output molecule is produced only when the correct input profile of miRNAs is detected. The genetic circuits and cell state classifiers may be used in various applications (e.g., therapeutic or diagnostic applications).

公开(公告)号：US11351271B2

公开(公告)日：2022-06-07

申请号：US15509258

申请日：2015-09-08

Applicant: Massachusetts Institute of Technology ,  Kyoto University

Inventor： Ron Weiss ,  Liliana Wroblewska ,  Velia Siciliano ,  Tasuku Kitada ,  Maria Hottelet Foley ,  Katie Bodner ,  Hirohide Saito ,  Kei Endo ,  Darrell J. Irvine ,  Tyler Wagner ,  Jacob Becraft

IPC: C12N15/11 ,  A61K48/00 ,  C12N15/63 ,  C12N15/10 ,  C12N15/85

Abstract: Engineered synthetic RNA-based genetic circuits are provided that are regulated exclusively at the post-transcriptional level.

公开(公告)号：US20210324414A1

公开(公告)日：2021-10-21

申请号：US17232676

申请日：2021-04-16

Applicant: Massachusetts Institute of Technology ,  University of Massachusetts

Inventor： Ron Weiss ,  Jin Huh ,  Yingzhong Li ,  Eric Goldstein ,  Hava Siegelmann

IPC: C12N15/86 ,  C07K16/10 ,  C12N15/113 ,  C12N15/11 ,  C12N9/22 ,  A61K9/51 ,  A61K35/28 ,  A61P31/14 ,  C12N15/10

Abstract: This disclosure provides engineered genetic systems for sequestering and/or destroying viruses, compositions and cells comprising the genetic systems, and methods of treating viral infections, reducing viral load, and/or reducing viral spread. The disclosure also provides libraries comprising elements to be incorporated into the engineered genetic systems.

公开(公告)号：US10731153B2

公开(公告)日：2020-08-04

申请号：US15410875

申请日：2017-01-20

Applicant: Massachusetts Institute of Technology ,  INRIA (French Institute for Research in Computer Science and Automation)

Inventor： Ron Weiss ,  Xavier Cesar Duportet ,  Gregory Batt ,  Yinqing Li

IPC: C12N15/00 ,  C12N15/10 ,  C12N15/85 ,  C12N9/22 ,  C12N15/52 ,  C12Q1/02 ,  C12Q1/6897

Abstract: The disclosure provides novel large serine recombinases and their respective recognition sites, as well as libraries of orthogonal recombinase recognition sites. Uses of the large serine recombinases, recognition sites, and libraries of orthogonal recombinase recognition sites also are provided.

公开(公告)号：US12297506B2

公开(公告)日：2025-05-13

申请号：US17688985

申请日：2022-03-08

Applicant: Massachusetts Institute of Technology

Inventor： Ron Weiss ,  Jin Huh

IPC: C12Q1/6886 ,  A61K31/7125 ,  C12N15/113 ,  C12Q1/6811 ,  C12Q1/6897

Abstract: Provided herein are genetic circuits and cell state classifiers for detecting the microRNA profile of a cell. The cell state classifiers of the present disclosure are designed to incorporate multiple genetic circuits integrated together by transcriptional or translational control. Multiple inputs can be sensed simultaneously by coupling their detection to different portions of the genetic circuit such that the output molecule is produced only when the correct input profile of miRNAs is detected. The genetic circuits and cell state classifiers may be used in various applications (e.g., therapeutic or diagnostic applications).

公开(公告)号：US12286646B2

公开(公告)日：2025-04-29

申请号：US16739182

申请日：2020-01-10

Applicant: Massachusetts Institute of Technology

Inventor： Darrell J. Irvine ,  Ron Weiss ,  Yingzhong Li

IPC: C12N7/00 ,  A61K47/69 ,  A61K49/00 ,  C12N9/02 ,  C12N15/10 ,  C12N15/86

Abstract: The disclosure provides methods for an in vitro evolution technique to identify and characterize mutations in the non-structural genes of an alphavirus replicon that increase the strength and persistence of expression of the replicon genome. Also provided herein are in vivo methods for administering to an animal model a mutated alphavirus replicon that codes for a gene of experimental or therapeutic interest in the subgenome of the alphavirus replicon. The mutations identified herein improve the therapeutic potential of self-replicating RNA, which may have implications for cancer immunotherapy and beyond, e.g., for vaccination or gene therapy.

公开(公告)号：US11834659B2

公开(公告)日：2023-12-05

申请号：US17032233

申请日：2020-09-25

Applicant: Massachusetts Institute of Technology

Inventor： Ron Weiss ,  Giulio Alighieri

IPC: C12N15/11 ,  C12N15/113 ,  A61K31/7105 ,  C12N9/22

CPC classification number: C12N15/113 ,  A61K31/7105 ,  C12N9/22 ,  C12N2310/122 ,  C12N2310/141 ,  C12N2310/20

Abstract: The present disclosure, at least in part, relates to an engineered RNA (e.g., microRNA and sgRNA), in the absence of an input signal, that is engineered to have a large enough energy gap between the formations of a first secondary structure, which is unrecognizable by an actuator, and a second secondary structure, which is recognizable by an actuator (e.g., Drosha and Cas protein).

公开(公告)号：US11492622B2

公开(公告)日：2022-11-08

申请号：US17032259

申请日：2020-09-25

Applicant: Massachusetts Institute of Technology

Inventor： Ron Weiss ,  Giulio Alighieri

IPC: C12N15/113

Abstract: The present disclosure, at least in part, relates to a miRNA based logic gate that comprises an engineered RNA carrier that comprises an nuclear export signal, a target site for a first miRNA and a pre-miRNA sequence for a second miRNA. Also provided by the disclosure are recombinant viruses (e.g., recombinant adeno-associated viruses (rAAV)) for delivery of the miRNA based logic gates.