公开(公告)号：US20130273521A1

公开(公告)日：2013-10-17

申请号：US13697291

申请日：2011-05-11

Applicant: Martin Lee ,  Mark Laverick

Inventor： Martin Lee ,  Mark Laverick

IPC: C12Q1/68 ,  C12Q1/70

CPC classification number: C12Q1/6818 ,  C12Q1/701 ,  C12Q2565/101 ,  C12Q2533/101 ,  C12Q2527/107 ,  C12Q2525/197

Abstract: The invention concerns a system for detecting a target nucleic acid molecule of a particular sequence in a sample, said system comprising (i) an oligonucleotide primer complementary to a said target nucleic acid molecule, which primer has no internal complementarity, is able to amplify said target sequence and carries a first label linked to said oligonucleotide at its 5′ end; and (ii) an oligonucleotide probe which carries a second label that is able to interact with said first label to produce a detectable signal, wherein the oligonucleotide probe binds an extension product of said primer such that the first and second label can interact to produce a detectable signal. Methods for using said system in particular in a nucleic acid assay, kits comprising the system and elements of it form a further aspect of the invention.

Abstract translation: 本发明涉及用于检测样品中特定序列的靶核酸分子的系统，所述系统包含（i）与所述靶核酸分子互补的寡核苷酸引物，该引物不具有内部互补性，能够扩增所述 靶序列，并在其5'端携带与所述寡核苷酸连接的第一标记; 和（ii）携带能够与所述第一标记相互作用以产生可检测信号的第二标记的寡核苷酸探针，其中寡核苷酸探针结合所述引物的延伸产物，使得第一和第二标记可以相互作用以产生 可检测信号。 特别是在核酸测定中使用所述系统的方法，包含该系统及其元件的试剂盒形成本发明的另一方面。