公开(公告)号：US08034572B2

公开(公告)日：2011-10-11

申请号：US11512408

申请日：2006-08-30

申请人： Mart Saarma ,  Heikki Rauvala ,  Maxim Bespalov ,  Sarka Tumova

发明人： Mart Saarma ,  Heikki Rauvala ,  Maxim Bespalov ,  Sarka Tumova

IPC分类号： G01N33/68

CPC分类号： G01N33/543 ,  G01N33/5308 ,  G01N2333/4722 ,  G01N2500/02 ,  G01N2800/28

摘要： The present invention relates to an in vitro method for identifying a molecule, which interferes with the interaction between a glial cell-line derived neurotrophic factor family ligand (GFL) and a heparan sulfate proteoglycan (HSPG), by screening a library of molecules against a matrix anchored complex comprising at least one immobilized glial cell-line derived neurotrophic factor family ligand (GFL) and at least one heparan sulfate proteoglycan (HSPG), wherein the interfering molecule is isolated based on its capacity to replace a glial cell-line derived neurotrophic factor family ligand (GFL) in said anchored complex. The invention also relates to a complex for identifying such a molecule. The invention also relates to methods for preventing or delaying a neurodegenerative process as well as to method for prophylactic treatment or treatment of a disorder in the nervous system.

摘要翻译： 本发明涉及一种用于鉴定分子的体外方法，该分子干扰胶质细胞系衍生的神经营养因子家族配体（GFL）和硫酸乙酰肝素蛋白多糖（HSPG）之间的相互作用，通过筛选分子文库 基质锚定复合物，其包含至少一种固定的胶质细胞系衍生的神经营养因子家族配体（GFL）和至少一种硫酸乙酰肝素蛋白多糖（HSPG），其中所述干扰分子基于其代替胶质细胞系衍生的神经营养 因子家族配体（GFL）。 本发明还涉及用于鉴定这种分子的复合物。 本发明还涉及用于预防或延迟神经变性过程的方法以及用于预防性治疗或治疗神经系统疾病的方法。

公开(公告)号：US20080057516A1

公开(公告)日：2008-03-06

申请号：US11512408

申请日：2006-08-30

申请人： Mart Saarma ,  Heikki Rauvala ,  Maxim Bespalov ,  Sarka Tumova

发明人： Mart Saarma ,  Heikki Rauvala ,  Maxim Bespalov ,  Sarka Tumova

IPC分类号： G01N33/567 ,  C12M1/34

CPC分类号： G01N33/543 ,  G01N33/5308 ,  G01N2333/4722 ,  G01N2500/02 ,  G01N2800/28

摘要： The present invention relates to an in vitro method for identifying a molecule, which interferes with the interaction between a glial cell-line derived neurotrophic factor family ligand (GFL) and a heparan sulfate proteoglycan (HSPG), by screening a library of molecules against a matrix anchored complex comprising at least one immobilized glial cell-line derived neurotrophic factor family ligand (GFL) and at least one heparan sulfate proteoglycan (HSPG), wherein the interfering molecule is isolated based on its capacity to replace a glial cell-line derived neurotrophic factor family ligand (GFL) in said anchored complex. The invention also relates to a complex for identifying such a molecule. The invention also relates to methods for preventing or delaying a neurodegenerative process as well as to method for prophylactic treatment or treatment of a disorder in the nervous system.

摘要翻译： 本发明涉及一种用于鉴定分子的体外方法，该分子干扰胶质细胞系衍生的神经营养因子家族配体（GFL）和硫酸乙酰肝素蛋白多糖（HSPG）之间的相互作用，通过筛选分子文库 基质锚定复合物，其包含至少一种固定的胶质细胞系衍生的神经营养因子家族配体（GFL）和至少一种硫酸乙酰肝素蛋白多糖（HSPG），其中所述干扰分子基于其代替胶质细胞系衍生的神经营养 因子家族配体（GFL）。 本发明还涉及用于鉴定这种分子的复合物。 本发明还涉及用于预防或延迟神经变性过程的方法以及用于预防性治疗或治疗神经系统疾病的方法。