公开(公告)号：US09109216B2

公开(公告)日：2015-08-18

申请号：US13497987

申请日：2010-09-23

申请人： Mark Ellis ,  David Paul Humphreys

发明人： Mark Ellis ,  David Paul Humphreys

IPC分类号： C12P21/02 ,  C12N9/52 ,  C12N15/70 ,  C07K16/24

CPC分类号： C12N1/20 ,  C07K16/241 ,  C07K16/244 ,  C07K2317/14 ,  C07K2317/20 ,  C07K2317/55 ,  C12N9/52 ,  C12N15/70 ,  C12P21/02 ,  C12R1/19 ,  Y02P20/52

摘要： A recombinant gram-negative bacterial cell comprising one or more of the following mutated protease genes: a) a mutated Tsp gene, wherein the mutated Tsp gene encodes a Tsp protein having reduced protease activity or is a knockout mutated Tsp gene; b) a mutated ptr gene, wherein the mutated ptr gene encodes a Protease III protein having reduced protease activity or is a knockout mutated ptr gene; and c) a mutated DegP gene encoding a DegP protein having chaperone activity and reduced protease activity; wherein the cell is isogenic to a wild-type bacterial cell except for the mutated Tsp gene and/or mutated ptr gene and/or mutated DegP gene and optionally a polynucleotide sequence encoding a protein of interest.

摘要翻译： 包含一个或多个以下突变蛋白酶基因的重组革兰氏阴性细菌细胞：a）突变的Tsp基因，其中突变的Tsp基因编码具有降低的蛋白酶活性的Tsp蛋白或是敲除突变的Tsp基因; b）突变的ptr基因，其中突变的ptr基因编码具有降低的蛋白酶活性的蛋白酶III蛋白质或是敲除突变的ptr基因; 和c）编码具有伴侣活性和降低的蛋白酶活性的DegP蛋白的突变DegP基因; 其中所述细胞与野生型细菌细胞是同基因的，除了突变的Tsp基因和/或突变的ptr基因和/或突变的DegP基因和任选的编码目的蛋白质的多核苷酸序列。

公开(公告)号：US08969037B2

公开(公告)日：2015-03-03

申请号：US13522080

申请日：2011-01-13

申请人： Mark Ellis ,  David Paul Humphreys

发明人： Mark Ellis ,  David Paul Humphreys

IPC分类号： C12N15/70 ,  C12N15/09 ,  C12N9/52 ,  C07K16/24 ,  C12P21/02

CPC分类号： C07K16/241 ,  C07K2317/14 ,  C07K2317/55 ,  C12N1/20 ,  C12N9/52 ,  C12N15/70 ,  C12P21/02 ,  Y02P20/52

摘要： The present invention provides a recombinant gram-negative bacterial cell comprising a mutant spr gene encoding a mutant spr protein and wherein the cell comprises a non-recombinant wild-type chromosomal Tsp gene.

摘要翻译： 本发明提供了一种重组革兰氏阴性细菌细胞，其包含编码突变型spr蛋白的突变型spr基因，并且其中所述细胞包含非重组野生型染色体Tsp基因。

公开(公告)号：US08204778B2

公开(公告)日：2012-06-19

申请号：US11771704

申请日：2007-06-29

申请人： Jason-Jno Engene Taylor ,  Mark Ellis

发明人： Jason-Jno Engene Taylor ,  Mark Ellis

IPC分类号： G06Q10/00

CPC分类号： G06Q10/1053 ,  G06Q10/063118 ,  G06Q10/0639 ,  G06Q10/06393 ,  G06Q10/10 ,  G06Q30/02 ,  G06Q30/0203

摘要： A method of selecting potential hires for progression through a hiring process is provided. The method comprises selecting at least one performance indicator for a position, obtaining the at least one performance indicator for a plurality of incumbents in the position, and placing each incumbent in the plurality of incumbents along a performance continuum according to their performance indicator. The method also comprises surveying incumbents from at least both halves of the performance continuum, generating an ideal profile for the position based upon the incumbent survey and the at least one performance indicator of the incumbents, using a survey to generate a candidate profile of a potential hire, comparing the candidate profile of the potential hire with the ideal profile for the position to determine a result for the potential hire, and determining whether to progress the potential hire to a next stage in the hiring process based upon the result.

摘要翻译： 提供了通过招聘过程选择潜在雇员进行进展的方法。 该方法包括为位置选择至少一个绩效指标，获得位于该位置的多个在职者的至少一个绩效指标，以及根据其绩效指标将每个在职者放置在执行连续统计中。 该方法还包括从绩效连续体的至少两个角度对在职人员进行调查，根据现任调查和现任人员的至少一个绩效指标，为职位创造理想的档案，使用调查来生成潜在的候选人资料 雇用，将潜在雇用的候选人资料与职位的理想档案进行比较，以确定潜在雇用的结果，以及根据结果确定是否将潜在雇用晋升到招聘过程中的下一阶段。

公开(公告)号：US20120040401A1

公开(公告)日：2012-02-16

申请号：US13203182

申请日：2010-02-25

申请人： Mark Ellis ,  Laura Ellen Newnham

发明人： Mark Ellis ,  Laura Ellen Newnham

IPC分类号： C12P21/00 ,  C12N1/19 ,  C07K16/46 ,  C12N5/10 ,  C12N15/63 ,  C12N15/64

CPC分类号： C07K16/00

摘要： The invention relates to a transcriptionally active recombinant linear polynucleotide encoding a multimeric protein comprising in the following order, a first promoter sequence, a first encoding polynucleotide sequence, a bidirectional regulatory sequence, a second encoding polynucleotide sequence and a second promoter sequence, wherein the first and second encoding polynucleotide sequences are in convergent transcriptional orientation, each encoding polynucleotide sequence encodes a component of the multimeric protein and the bidirectional regulatory sequence is operably linked to the first and second encoding polynucleotide sequences and the multimeric protein is an antibody or fragment thereof and each encoding polynucleotide sequence encodes one or more antibody domains or fragments thereof.

摘要翻译： 本发明涉及编码多聚蛋白的转录活性重组线性多核苷酸，其包含以下顺序，第一启动子序列，第一编码多核苷酸序列，双向调节序列，第二编码多核苷酸序列和第二启动子序列，其中第一 并且第二编码多核苷酸序列处于会聚转录取向，每个编码多核苷酸序列编码多聚体蛋白的组分，并且双向调节序列可操作地连接到第一和第二编码多核苷酸序列，多聚体蛋白是抗体或其片段，并且每个 编码多核苷酸序列编码一个或多个抗体结构域或其片段。

公开(公告)号：US20070131864A1

公开(公告)日：2007-06-14

申请号：US11548559

申请日：2006-10-11

申请人： Mark Ellis ,  Son Le ,  Larry Davis

发明人： Mark Ellis ,  Son Le ,  Larry Davis

IPC分类号： G01J5/02

CPC分类号： G01N21/05 ,  G01N21/3504 ,  G01N21/6428 ,  G01N21/783

摘要： A system for detecting and remediating a discharge of fuel from a fuel source facility is disclosed. The system can comprise a contamination detection subsystem configured to detect a discharge of fuel from the fuel source facility and a remediation subsystem configured to remediate contamination due to the discharge of fuel. A controller can automatically activate the remediation subsystem upon detection of a discharge by the contamination detection subsystem. The controller can automatically send a notification that a contamination has been detected and the remediation system has been activated. Alternatively or in addition, the controller can automatically send a notification that a contamination has been detected and the remediation system has been activated.

摘要翻译： 公开了一种用于从燃料源设备检测和补救燃料排放的系统。 该系统可以包括被配置为检测来自燃料源设备的燃料排放物的污染检测子系统和被配置为修复由于燃料排放引起的污染物的修复子系统。 检测到污染检测子系统的放电时，控制器可以自动激活补救子系统。 控制器可以自动发送已经检测到污染的通知，并且修复系统已被激活。 或者或另外，控制器可以自动发送已经检测到污染并且补救系统已被激活的通知。

公开(公告)号：US20240046906A1

公开(公告)日：2024-02-08

申请号：US18381312

申请日：2023-10-18

申请人： Mark Ellis

发明人： Mark Ellis

IPC分类号： G10H3/18 ,  G10H1/08 ,  G10D15/00 ,  G10D1/08 ,  G10H1/14

CPC分类号： G10H3/186 ,  G10H3/185 ,  G10H3/182 ,  G10H3/181 ,  G10H1/08 ,  G10D15/00 ,  G10D1/08 ,  G10H1/14 ,  G10H2210/565 ,  G10H2220/515 ,  G10H2210/311

摘要： A system and a kit can be used for onboard, real-time blending for electronic pickups to produce various tonal combinations. In one example, at least one of the electronic pickups has dual coils. The dual coil pickup can be electrically connected to a switching circuit positioned on a stringed instrument. The switching circuit can be adjustable in real-time to select between at least two conditions for a signal of the dual coil pickup. In one instance, the conditions can select north coil only, series wiring of the dual coils, or south coil only. Alternatively, the conditions can select series wiring or parallel wiring of the dual coils. Each of the electronic pickups is electrically connected to a taper circuit positioned on the instrument. Each taper circuit is adjustable in real-time to vary an amplitude of the signal from the respective electronic pickup for output of the instrument.

公开(公告)号：US08484072B1

公开(公告)日：2013-07-09

申请号：US13460117

申请日：2012-04-30

申请人： Jason-Jno Eugene Taylor ,  Mark Ellis

发明人： Jason-Jno Eugene Taylor ,  Mark Ellis

IPC分类号： G06Q10/00

CPC分类号： G06Q10/1053 ,  G06Q10/063118 ,  G06Q10/0639 ,  G06Q10/06393 ,  G06Q10/10 ,  G06Q30/02 ,  G06Q30/0203

摘要： A method of selecting potential hires for progression through a hiring process is provided. The method comprises selecting at least one performance indicator for a position, obtaining the at least one performance indicator for a plurality of incumbents in the position, and placing each incumbent in the plurality of incumbents along a performance continuum according to their performance indicator. The method also comprises surveying incumbents from at least both halves of the performance continuum, generating an ideal profile for the position based upon the incumbent survey and the at least one performance indicator of the incumbents, using a survey to generate a candidate profile of a potential hire, comparing the candidate profile of the potential hire with the ideal profile to determine a result for the potential hire, and determining whether to progress the potential hire to a next stage in the hiring process based upon the result.

摘要翻译： 提供了通过招聘过程选择潜在雇员进行进展的方法。 该方法包括为位置选择至少一个绩效指标，获得位于该位置的多个在职者的至少一个绩效指标，以及根据其绩效指标将每个在职者放置在执行连续统计中。 该方法还包括从绩效连续体的至少两个角度对在职人员进行调查，根据现任调查和现任人员的至少一个绩效指标，为职位创造理想的档案，使用调查来生成潜在的候选人资料 雇用，将潜在雇用的候选人资料与理想档案进行比较，以确定潜在雇用的结果，以及根据结果确定是否将招聘过程中的潜在雇用晋升到下一阶段。

公开(公告)号：US20070191506A1

公开(公告)日：2007-08-16

申请号：US11276068

申请日：2006-02-13

申请人： Ying-Yuh Lu ,  Jianhui Xia ,  Peter Olofson ,  Ming Cheng ,  Mark Ellis ,  Babu Gaddam

发明人： Ying-Yuh Lu ,  Jianhui Xia ,  Peter Olofson ,  Ming Cheng ,  Mark Ellis ,  Babu Gaddam

IPC分类号： B29D11/00

CPC分类号： C09D151/003 ,  C08F2/48 ,  C08F265/04 ,  C08F265/06 ,  C08F290/06 ,  C08F290/061 ,  C08L51/003 ,  C08L2666/02 ,  C09J151/003 ,  G02B1/041 ,  Y10T428/10 ,  C08L33/00

摘要： A curable composition is provided comprising an oligomer having a plurality of pendent and/or terminal ethylenically unsaturated, free-radically polymerizable functional groups, a free-radically polymerizable crosslinking agent, and/or a diluent monomer, and a photoinitiator. The composition, when cured, is non-yellowing, exhibits low shrinkage and low birefringence making it suitable for many optical applications such as optical lenses, optical fibers, prisms, light guides, optical adhesives, and optical films.

摘要翻译： 提供了一种可固化组合物，其包含具有多个侧链和/或末端烯键式不饱和，可自由基聚合的官能团，可自由基聚合的交联剂和/或稀释剂单体和光引发剂的低聚物。 当固化时，组合物不变黄，显示低收缩率和低双折射，使其适用于许多光学应用，例如光学透镜，光纤，棱镜，光导，光学粘合剂和光学膜。

公开(公告)号：US20070142579A1

公开(公告)日：2007-06-21

申请号：US11275165

申请日：2005-12-16

申请人： Mark Ellis

发明人： Mark Ellis

IPC分类号： C08F4/04

CPC分类号： C08F2/00 ,  C08F6/006 ,  C08F218/10 ,  C08F220/14 ,  C08F220/18 ,  C08F220/30 ,  C08L33/06

摘要： A method for the polymerization of free radically polymerizable vinyl monomers involves polymerizing free radically (co)polymerizable vinyl monomers in a batch reaction under essentially adiabatic conditions, and utilizing scavenger monomer to reduce undesirable residual monomer.

摘要翻译： 用于自由基聚合的乙烯基单体的聚合方法包括在基本上绝热的条件下在分批反应中自由基（共）可聚合的乙烯基单体聚合，并且利用清除剂单体以减少不需要的残余单体。

公开(公告)号：US20060256568A1

公开(公告)日：2006-11-16

申请号：US11130454

申请日：2005-05-16

申请人： Mark Ellis

发明人： Mark Ellis

IPC分类号： F21S13/10

CPC分类号： F21V21/0824 ,  F21S8/081 ,  F21S9/02 ,  F21V21/22 ,  F21W2131/10 ,  F21W2131/109 ,  F21Y2115/10

摘要： The Portable Outdoor LED Illumination Apparatus provides nighttime illumination of an outdoor area for campers and other outdoor enthusiasts. The apparatus includes a vertical, tubular support pole and an illumination head assembly. The vertical, tubular support pole is comprised of a plurality of segments which are connected via springs internal to the segments. The springs allow easy collapse of the vertical, tubular support pole into a transportable unit. The segments, and therefore the vertical tubular support pole, are hollow and allow for an electrical conductor to pass from a compartment housing a plurality of batteries to the illumination head assembly. The illumination head assembly, which is comprised of a plurality of light emitting diodes (“LED's”) fixed within an epoxy potting, is affixed in such a manner so that when the LED's are powered the light is directed in the general direction of the ground.

摘要翻译： 便携式室外LED照明装置为露营者和其他户外爱好者提供户外区域的夜间照明。 该装置包括垂直的管状支撑杆和照明头组件。 垂直管状支撑杆由多个段组成，这些段通过节段内部的弹簧连接。 弹簧允许垂直的管状支撑杆容易地折叠成可移动单元。 段和因此垂直管状支撑杆是中空的，并且允许电导体从容纳多个电池的隔室通过到照明头组件。 固定在环氧树脂灌封中的多个发光二极管（“LED”）组成的照明头组件以这样的方式固定，使得当LED被供电时，光被指向地面的大致方向 。