公开(公告)号：US20240003792A1

公开(公告)日：2024-01-04

申请号：US17928177

申请日：2021-04-19

Applicant: MDimune Inc.

Inventor： Shin Gyu Bae ,  Seung Wook Oh ,  Dong Woo Han

IPC: G01N1/40

CPC classification number: G01N1/4077 ,  G01N2001/4088

Abstract: Provided is a method for preparing cell-derived vesicles, and more particularly, a method for preparing cell-derived vesicles using a cell extruder, and a syringe-type cell extruder for effectively preparing cell-derived vesicles. According to the present invention, by using the method for preparing the cell-derived vesicles and the cell extruder, it is possible to prepare cell-derived vesicles in a stable, economical, and mass-producible manner.

公开(公告)号：US20240417696A1

公开(公告)日：2024-12-19

申请号：US17927335

申请日：2021-05-17

Applicant: MDimune Inc.

Inventor： Shin Gyu Bae ,  Seung Wook Oh ,  Dong Woo Han ,  Jun Sik Yoon ,  Ji Hye Lee

IPC: C12N5/09 ,  A61K9/16 ,  A61K9/20 ,  A61K9/48 ,  A61K35/13 ,  A61K35/17 ,  A61K35/28 ,  C12N5/0775 ,  C12N5/0783

Abstract: The present disclosure relates to a cell-derived vesicle rich in intracellular protein homeostasis regulators, and to a method of preparing same. The cell-derived vesicle of the present disclosure is characterized by containing an abundance of a specific protein compared to that in a naturally secreted exosome or the originated cell, and by virtue of said characteristic, can be utilized for various purposes such as in a pharmaceutical composition, a diagnostic composition, and a composition for drug delivery. Using the cell-derived vesicle rich in intracellular protein homeostasis regulators and the method of preparing same of the present disclosure, it is possible to easily deliver the intracellular protein homeostasis regulators to a target, such as a cell, thereby easily maintaining protein homeostasis in cells. Accordingly, the present disclosure may also be utilized for “a use for the prevention or treatment of disease caused by a protein homeostasis regulation failure within cells” or “a use for preventing aging”, and thus can be widely used in the drug industry.