公开(公告)号：US20140051584A1

公开(公告)日：2014-02-20

申请号：US13967665

申请日：2013-08-15

Applicant: Life Technologies Corporation

Inventor： Melville DAVEY ,  Michael MEYER ,  Marcin SIKORA ,  Simon CAWLEY ,  Kirk PASTORIAN

IPC: C12Q1/68

CPC classification number: C12Q1/6874 ,  G06F19/10 ,  C12Q2537/165

Abstract: A method of estimating a parameter related to sequencing of a sample nucleic acid template includes: receiving signal data relating to nucleotide incorporation events resulting from a series of flows of nucleotides onto an array of wells including (i) a first well containing the sample nucleic acid template and (ii) a plurality of other sample-containing wells; determining sequence information for the sample nucleic acid template using signal data from the first well; and constructing a phase-state model for a set of nucleotide flows that contributed at least in part to the sequence information, wherein the model includes a signal correction parameter that is determined using signal data from the plurality of other sample-containing wells.

Abstract translation: 估计与样品核酸模板的测序有关的参数的方法包括：接收与一系列核苷酸产生的核苷酸掺入事件相关的信号数据，其包含（i）包含样品核酸的第一阱 模板和（ii）多个其他含样品的孔; 使用来自第一孔的信号数据确定样品核酸模板的序列信息; 以及构建至少部分地对所述序列信息贡献的一组核苷酸流的相态模型，其中所述模型包括使用来自所述多个其它含样品孔的信号数据确定的信号校正参数。